Variation in the ovine PRKAG3 gene

Yang, Guo; Zhou, Huitong; Wang, Ruoyu; Hickford, Jonathan G. H.

doi:10.1016/j.gene.2015.05.021

Cited by 4 publications

(4 citation statements)

References 22 publications

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“…PRKAG3 encodes the γ3 subunit of the 5' AMP-activated protein kinase (AMPK) that controls cellular energy homeostasis in response to environmental or nutritional stress. Variation in this gene has been found to be associated with meat quality traits by affecting meat pH and tenderness in cattle [31] and sheep [32]. But similar studies have not been reported in goat yet.…”

Section: Discussionmentioning

confidence: 92%

Multiomics analyses of Jining Grey goat and Boer goat reveal genomic regions associated with fatty acid and amino acid metabolism and muscle development

Liu,

Tan,

Jin

et al. 2024

Anim Biosci

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Objective: Jining Grey goat is a local Chinese goat breed that is well known for its high fertility and excellent meat quality but shows low meat production performance. Numerous studies have focused on revealing the genetic mechanism of its high fertility, but its highlighting meat quality and muscle growth mechanism still need to be studied.Methods: In this research, an integrative analysis of the genomics and transcriptomics of Jining Grey goats compared with Boer goats was performed to identify candidate genes and pathways related to the mechanisms of meat quality and muscle development.Results: Our results overlap among five genes (ABHD2, FN1, PGM2L1, PRKAG3, RAVER2) and detected a set of candidate genes associated with fatty acid metabolism (PRKAG3, HADHB, FASN, ACADM), amino acid metabolism (KMT2C, PLOD3, NSD2, SETDB1, STT3B, MAN1A2, BCKDHB, NAT8L, P4HA3) and muscle development (MSTN, PPARGC1A, ANKRD2). Several pathways have also been detected, such as the FoxO signaling pathway and Apelin signaling pathway that play roles in lipid metabolism, lysine degradation, N-glycan biosynthesis, valine, leucine and isoleucine degradation that involving with amino acid metabolism.Conclusion: The comparative genomic and transcriptomic analysis of Jining Grey goat and Boer goat revealed the mechanisms underlying the meat quality and meat productive performance of goats. These results provide valuable information for future breeding of goats.

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Section: Discussionmentioning

confidence: 92%

Multiomics analyses of Jining Grey goat and Boer goat reveal genomic regions associated with fatty acid and amino acid metabolism and muscle development

Liu,

Tan,

Jin

et al. 2024

Anim Biosci

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“…Having confirmed that CO II gene sequences can be used to accurately classify Diatraea species (considering the genitalia-based key as a gold standard), a molecular method was developed that does not require sequencing of PCR fragments in order to accelerate the time to obtain results, as well as to increase the number of samples that can be analyzed. Thus, a PCR-SSCP methodology was selected for its high potential revealed in variability studies [ 39 – 41 ], and the results were compared with genitalia analyzes and bioinformatics studies based on CO II. In first place, the most favorable secondary structure of sense and antisense DNA strands from PCR products of CO II were predicted ( Fig 5 ).…”

Section: Resultsmentioning

confidence: 99%

Identification of Diatraea spp. (Lepidoptera: Crambidae) based on cytochrome oxidase II

et al. 2017

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Diatraea spp. (Lepidoptera: Crambidae) are a group of insects that are agriculture pests in many economically relevant crops such as sugarcane, sorghum, corn and rice. Recognized species for this genus respond differentially to natural enemies used in their biological control, emphasizing the importance of species in a regional approach. Currently, identification is based on the male genitalia. However, the availability of specimens collected from field and subjectivity based on the character recognition can seriously hamper species identification, and therefore result in inadequate pest management. To overcome this, individuals of Diatraea spp. preliminarily classified male genitalia and obtained from reared conditions and the field (both derived from natural populations occurring in Colombia) were analyzed using genitalic morphometry and molecular biology specifically using a fragment of the cytochrome oxidase subunit II (CO II) mitochondrial gene. Although morphometric analysis did not show any overriding results regarding genitalia morphology, the bioinformatics analyses of CO II sequences resulted in an adequate classification of the individuals within the recognized species. It also, revealed that the occurrence of clades associated with geographical distribution may be associated with cryptic species. The latter was also confirmed by a Single-Strand Conformation Polymorphism (SSCP) methodology evaluating the same fragment of CO II. This experimental approach allows properly recognizing each species and in consequence is proposed as an effective tool in Diatraea species identification.

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“…We also genotyped three SNPs of the PRKAG3 gene and evidenced that SNP rs159573167 AA was associated with an increase of dMY in the sampled population (+17% in comparison with genotype GG; data not shown in the tables) and a decrease of the protein and casein contents. The variability at the PRKAG3 gene was investigated in New Zealand Suffolk sheep [ 47 ], and the variability and expression profile in skeletal muscle were investigated in five local Spanish breeds, where PRKAG3 was found to be highly expressed [ 48 ]. Here, for the first time, we highlighted an association between PRKGA3 and dMY and the protein and casein contents.…”

Section: Discussionmentioning

confidence: 99%

Novel Genes Associated with Dairy Traits in Sarda Sheep

Pazzola

Vacca

Paschino

et al. 2021

Animals

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The aim of the present research was to analyze the variability of 45 SNPs from different genes involved in metabolism and innate immunity to perform an association analysis with the milk yield, composition and milk coagulation traits. A population of 1112 Sarda breed sheep was sampled. Genotyping was generated by a TaqMan Open ArrayTM. Thirty out of the 45 SNPs were polymorphic, and 12 displayed a minor allele frequency higher than 0.05. An association analysis showed that the variability at genes PRKAG3 and CD14 was significantly associated with the daily milk yield. The variability at PRKAG3 was also associated with the protein and casein content, somatic cell score and bacterial score. The variation at the PRKAA2 gene was associated with the milk lactose concentration. The SNPs at CD14 were also associated with the traditional milk coagulation properties, while the SNPs at GHR and GHRHR were associated with kSR, a derived coagulation parameter related to the rate of syneresis. The information provided here is new and increases our knowledge of genotype–phenotype interactions in sheep. Our findings might be useful in appropriate breeding schemes to be set up for the Sarda sheep breed, but these should be confirmed by further studies, possibly performed on independent populations.

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Variation in the ovine PRKAG3 gene

Cited by 4 publications

References 22 publications

Multiomics analyses of Jining Grey goat and Boer goat reveal genomic regions associated with fatty acid and amino acid metabolism and muscle development

Multiomics analyses of Jining Grey goat and Boer goat reveal genomic regions associated with fatty acid and amino acid metabolism and muscle development

Identification of Diatraea spp. (Lepidoptera: Crambidae) based on cytochrome oxidase II

Novel Genes Associated with Dairy Traits in Sarda Sheep

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