Variation in the Forkhead Box Class O3 (Foxo3) Gene and Its Association With Lifespan Traits in Barki Ewes

Ibrahim, A. H. M.; Alsheikh, S. M.

doi:10.21608/ejgc.2014.9925

Cited by 2 publications

(2 citation statements)

References 32 publications

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“…Noting that the current study indicated a high percentage of the hybrid composition, and this The ratios vary between studies conducted by researchers on other sheep breeds, as it appeared in [19]. (CC), and also appeared in [23]. three alleles of the same gene with four genotypes (AA, AB, AC, CC) and the absence of the genotype BC.…”

Section: Genotype Ratios Of the C327t Mutation Of The Foxo3 Genementioning

confidence: 38%

Detecting Polymorphisms of Mutation (C327T) rs 417415931 of Gene Foxo3 and its Associations with Reproductive Performance of Awassi Sheep

Ibadi,

Senkal

2023

IOP Conf. Ser.: Earth Environ. Sci.

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The experimental samples for the current study included 45 sheep of the local Awassi breed, whose ages ranged between (2-4) years. These ewes produced 54 newborns, and their ages ranged from birth to the sixth month, and for the period from 1/2/2022 to 25/6/2022. The experiment was conducted. For this study, the field side was carried out at the Al-Fayhaa cow farm and station of the Directorate of Agriculture of Babylon / Ministry of Agriculture. As for the laboratory side, laboratory analyzes were conducted in the laboratories of the Scientific Advancement Company specialized in molecular genetics for the period from 1/5/2022 to 6/20/2022. With the aim of isolating the substance GeneticsDNA and the effect of the genetic morphology of the 417415931 (C327T) rs mutation and the study of the relationship of this genetic morphology with the productive traits represented in daily milk production (DMY), milk components, growth traits for lambs, traits related to maternal body dimensions, live weight of mothers and type of birth. This mutation was found in the plot. Studied with a molecular size of 365 bp) from exon number 2 for the Foxo3 gene, through the DNA nucleotide sequencing technique. This mutation produced three genotypes (CC, CT, and TT), with distribution ratios of (2.28, 36.36, 61.36)% sequentially, and the variance between these ratios was highly significant (p≤0.01), and the allelic frequency was (0.20, 0.80) for each of the C and T alleles. Respectively. It was found that the effect of the genotypes of the Foxo3 gene was not significant in the studied traits, as the ratios were close between the genotypes produced by the wild, the hybrid, and the pure genotype. It can be concluded from the results of this study not to rely on this mutation formed by the Foxo3 gene, and to adopt it as a genetic marker to improve productive traits or genital.

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Section: Genotype Ratios Of the C327t Mutation Of The Foxo3 Genementioning

confidence: 38%

Detecting Polymorphisms of Mutation (C327T) rs 417415931 of Gene Foxo3 and its Associations with Reproductive Performance of Awassi Sheep

Ibadi,

Senkal

2023

IOP Conf. Ser.: Earth Environ. Sci.

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“…These individuals outperformed the individuals carrying the CC mutant genotype. Noting that the current study indicated a high percentage of hybrid composition, and this The ratios vary between studies conducted by researchers on other sheep breeds, as it appeared in [ 14] of their studies about newzeland Sheep three alleles for this gene A,B, and C with five genotypes (AA,AB,AC,BC,CC).And it appeared for [18] three alleles of the same gene with four genotypes (AA, AB, AC, CC) and the absence of the genotype BC. The difference that appears in the distribution ratios between studies may be due to the differences between the strains as well as factors related to nutrition, or it may be due to other genetic factors such as the influence caused by modified genes [19].…”

Section: Genotype Ratios Of the C519t Mutation Of The Foxo3 Genementioning

confidence: 47%

Detecting Polymorphisms of Mutation (C519T) Sit 112480 of Gene Foxo3 and its Associations with Reproductive Performance of Awassi Sheep

Ibadi,

Senkal

2023

IOP Conf. Ser.: Earth Environ. Sci.

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The current study was conducted on a sample of 45 local Awassi ewe, with ages ranging from 2-4 years. These ewes produced 54 newborns, and the ages and weights of these newborns were monitored from birth until the sixth month of life for the period from 1/5/2022 to 6/25/2022 at Al-Fayhaa Sheep Station / Babylon Agricultural Directorate / Ministry of Agriculture. As for laboratory analyzes, they were conducted in the laboratories of the Scientific Advancement Company for Molecular Genetics for the period from 1/5/2022 to 6/25/2022. For the purpose of extracting DNA material, and determining the genotypes of the Foxo3 gene through the genotype of the mutation (Sit 112480)) T519C and studying the effect of this mutation on the studied characteristics, which are daily milk production (DMY), milk components, growth characteristics of lambs (different weights and ages), the rate of weight gain between weights mentioned above, growth rate, live mothers’ weights, mothers’ body dimensions, type of delivery (twins or singles). This formed mutation, which was found in the second segment of exon No. 2 of the Foxo3 gene, was amplified with a size of 420 bp N-hall using the nucleotide sequencing technique. This mutation produced three genotypes (TT, TC, and CC), and the ratios of genotypes were (0.00, 53.66, 46.34), sequentially. The variance between these ratios was highly significant (p≤0.01), and the allelic frequency was 0.27, 0.73 for both the T and C alleles. It was clear from the results that the effect of the different genotypes of the Foxo3 gene was not significant for the characteristic of daily milk production, and some other components of milk except for the percentage of fat were significant (p≤0.05). Weight between birth weight and weaning weight, and the results also show that there are significant differences for the type of birth characteristic (P≤0.05). There were no significant differences in the different genotypes of the Foxo3 gene for the characteristics represented by the body dimensions of the mothers and the live weight of the mothers, as well as the growth characteristics of the lambs, which are represented by the weight for the first three months of life and the rate of weight gain between them. It can be concluded from the previous results of this study the possibility of adopting the distinct genotypes of the Foxo3 gene for this genotype in the genetic improvement programs of the studied Awassi sheep to obtain the best economic return by selecting and cross-crossing the distinct genotypes in the characteristics that achieved the best ratios as well as the weight rates.

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Variation in the Forkhead Box Class O3 (Foxo3) Gene and Its Association With Lifespan Traits in Barki Ewes

Cited by 2 publications

References 32 publications

Detecting Polymorphisms of Mutation (C327T) rs 417415931 of Gene Foxo3 and its Associations with Reproductive Performance of Awassi Sheep

Detecting Polymorphisms of Mutation (C327T) rs 417415931 of Gene Foxo3 and its Associations with Reproductive Performance of Awassi Sheep

Detecting Polymorphisms of Mutation (C519T) Sit 112480 of Gene Foxo3 and its Associations with Reproductive Performance of Awassi Sheep

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