Variation in susceptibility of grapevine pruning wound to infection by <i>Eutypa lata</i> in south‐western France

Chapuis, L.; Richard, Luc; Dubos, B.

doi:10.1046/j.1365-3059.1998.00258.x

Cited by 38 publications

(40 citation statements)

References 18 publications

(33 reference statements)

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“…As it has been reported that wound susceptibility to trunk pathogens can be extended over long periods, from several weeks up to 3 months (Petzoldt et al 1981, Munkvold and Marois 1995, Chapuis et al 1998, Serra et al 2008, it is not surprising that positive correlations in our study were observed 2-3 months after the pruning events, which would indicate that wound infections accumulated over the whole 3-month period after the pruning time. In contrast, the coefficients corresponding to relative humidity were always negative and non-significant throughout the experimental period.…”

Section: Luque Et Al Natural Fungal Infections Of Pruning Wounds 139supporting

confidence: 70%

Natural infections of pruning wounds by fungal trunk pathogens in mature grapevines in Catalonia (Northeast Spain)

Luque

González-Peñas

García-Figueres

et al. 2013

Australian Journal of Grape and Wine Research

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Background and Aims Information on the natural infection rates of pruned canes caused by fungal trunk pathogens is scarce. This study aimed to determine the pathogenic mycoflora infecting the pruning wounds in two vineyards in Catalonia, Spain, each with a different level of trunk diseases, and in two pruning seasons. Methods and Results Vines were pruned in each vineyard in mid‐autumn leaving four to six buds. Three months later, pathogens were isolated and identified in 250 pruned canes chosen at random in each vineyard. Vines were then definitively pruned to two buds, and sampling for pathogen isolation and identification was repeated 3 months later. The main fungal pathogens identified in this study were Eutypa lata (0–0.4% of isolations), Neofusicoccum parvum (0–1.2%), Botryosphaeria dothidea (0–1.6%), Phomopsis spp. (0–1.6%), Cryptovalsa ampelina (0–3.2%), Phaeomoniella chlamydospora (0–12.0%) and Diplodia seriata (0.4–68.4%). A strong seasonal effect on pathogen infections was detected for most species, with a higher isolation percentage detected after the late pruning as compared with that of the early pruning. Conclusions Under the environmental conditions and the geographical location of this study, our results showed that the rate of natural infection of pruning wounds was lower following early pruning (autumn) than following late pruning (winter). Significance of the Study Early pruning could be used in combination with other control measures, such as chemical and biological wound protectants, to reduce the infections caused by the grapevine trunk pathogens during the pruning season in Catalonia, Spain. The infection risk, however, and potential effects of the early pruning on grape production should be considered in other environments before expanding this recommendation to other grapegrowing regions.

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Section: Luque Et Al Natural Fungal Infections Of Pruning Wounds 139supporting

confidence: 70%

Natural infections of pruning wounds by fungal trunk pathogens in mature grapevines in Catalonia (Northeast Spain)

Luque

González-Peñas

García-Figueres

et al. 2013

Australian Journal of Grape and Wine Research

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“…Coutos-Thévenot et al (2001) have shown that resveratrol limits the growth of E. lata in vitro while Del Río et al (2004) showed that phenolic compounds inhibited activity of its cell wall-degrading enzymes suggesting that phenolic compounds play a role in limiting wood colonisation in vivo. The accumulation of phenolic compounds in response to either E. lata or Trichoderma species has not been shown in grapevine wood despite extensive knowledge of the wound protective effect of pruning wound saprophytes, some of which have no direct antagonistic effect on the pathogens (Munkvold and Marois 1995;Chapuis et al 1998). However, the accumulation of phenolic compounds has been demonstrated in grapevine response to P. chlamydospora (Amalfitano et al 2011).…”

Section: Discussionmentioning

confidence: 99%

Response of Vitis vinifera cell cultures to Eutypa lata and Trichoderma atroviride culture filtrates: expression of defence-related genes and phenotypes

et al. 2016

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Cell suspension cultures of Vitis vinifera cv. Dauphine berries were used to study the response to the vascular pathogen, Eutypa lata, in comparison with a biological control agent, Trichoderma atroviride, that was previously shown to be effective in pruning wound protection. The expression of genes coding for enzymes of the phenylpropanoid pathway and pathogenesis-related (PR) proteins was profiled over a 48-h period using quantitative reverse transcriptase PCR. The cell cultures responded to elicitors of both fungi with a hypersensitive-like response that lead to a decrease in cell viability. Similar genes were triggered by both the pathogen and biocontrol agent, but the timing patterns and magnitude of expression was dependent on the specific fungal elicitor. Culture filtrates of both fungi caused upregulation of phenylalanine ammonia-lyase (PAL), 4-coumaroyl Co-A ligase (CCo-A) and stilbene synthase (STS), and a downregulation of chalcone synthase (CHS) genes. The pathogen filtrate caused a biphasic pattern in the upregulation of PAL and STS genes which was not observed in cells treated with filtrates of the biocontrol agent. Analytical assays showed significantly higher total phenolic content and chitinolytic enzyme activity in the cell cultures treated with the T. atroviride filtrate compared to the pathogen filtrate. These results corresponded well to the higher expression of PAL and chitinase class IV genes. The response of the cell cultures to T. atroviride filtrate provides support for the notion that the wound protection by the biocontrol agent at least partially relies on the induction of grapevine resistance mechanisms.

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“…Various studies have investigated the susceptibility of pruning wounds to Eutypa infections, and it is generally accepted that susceptibility is dependent on the time of pruning and the age of the pruning wound. Pruning wounds made early in the dormant season are much more susceptible and stay susceptible for much longer periods than pruning wounds made during the mid-and late dormancy periods (Petzoldt et al, 1981;Trese et al, 1982;Munkvold & Marois, 1995;Chapuis et al, 1998;John et al, 2005). The decrease in susceptibility is correlated with an increase in suberin and lignin deposition and with degree-day accumulation (Munkvold & Marois, 1995).…”

Section: Introductionmentioning

confidence: 99%

“…The decrease in susceptibility is correlated with an increase in suberin and lignin deposition and with degree-day accumulation (Munkvold & Marois, 1995). Populations of nonpathogenic microorganisms on wound surfaces are also more active and increase more rapidly during the mid-and late dormancy periods (Munkvold & Marois, 1995;Chapuis et al, 1998). Some of these microorganisms, which can occur naturally on grapevine pruning wounds, may have the ability to reduce E. lata infections (Ferreira et al, 1991;Munkvold & Marois, 1993b;Schmidt et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

Protection of Grapevine Pruning Wounds against Eutypa lata by Biological and Chemical Methods

Halleen

Fourie

Lombard³

2016

SAJEV

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Eutypa dieback, caused by the fungus Eutypa lata, is a serious disease of grapevines that infects mainly through pruning wounds. The aim of this study was to evaluate the in vitro efficacy of fungicides from various chemical groups against E. lata, as well as the in vivo efficacy of the most effective fungicides and selected bacterial and fungal antagonists of E. lata, in grapevine pruning wound protection trials. In vitro studies revealed that flusilazole, tebuconazole, benomyl, fenarimol and myclobutanil were the most effective fungicides to inhibit mycelial growth of E. lata. Two field trials were conducted, one subjected to artificial inoculation and the second to natural infection only. In the first, benomyl, flusilazole and commercially available Trichoderma harzianum-containing products and an experimental Bacillus subtilis strain were applied to fresh pruning wounds. Two Cabernet Sauvignon vineyards were pruned in August 2001 and 2002 and immediately treated and inoculated with a spore suspension of E. lata one day later. Isolations were made from the treated pruning wounds after 12 months to assess the effectiveness of the treatments. The fungicides benomyl and flusilazole were the most effective treatments, although the Trichoderma treatments T77 and Trichoseal spray caused a significant reduction in E. lata infection. In a second trial, pruning wounds of Cabernet Sauvignon, Sauvignon blanc, Red Globe and Bonheur were treated with the Trichoderma products Vinevax (= Trichoseal spray) and Eco77 (= T77) in August 2005 and 2006, subjected to natural infection only and evaluated after seven months. Vinevax and Eco77 not only reduced E. lata, but they also reduced the incidence of other grapevine trunk disease pathogens.

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Variation in susceptibility of grapevine pruning wound to infection by Eutypa lata in south‐western France

Cited by 38 publications

References 18 publications

Natural infections of pruning wounds by fungal trunk pathogens in mature grapevines in Catalonia (Northeast Spain)

Natural infections of pruning wounds by fungal trunk pathogens in mature grapevines in Catalonia (Northeast Spain)

Response of Vitis vinifera cell cultures to Eutypa lata and Trichoderma atroviride culture filtrates: expression of defence-related genes and phenotypes

Protection of Grapevine Pruning Wounds against Eutypa lata by Biological and Chemical Methods

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