Variation in properties of <i>Phlebiopsis gigantea</i> related to biocontrol against infection by <i>Heterobasidion</i> spp. in Norway spruce stumps

Sun, Hui; Korhonen, K.; Hantula, Jarkko; Kasanen, Risto

doi:10.1111/j.1439-0329.2008.00574.x

Cited by 34 publications

(37 citation statements)

References 21 publications

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“…The growth rate of Latvian P. gigantea isolates on agar medium at room temperature varied from 5.1 to 8.6 mm day -1 . Within the material investigated by Sun et al (2009a), consisting of 64 P. gigantea isolates, the corresponding variation was from 3.8 to 10.8 mm day -1 . Abundant sporulation of a P. gigantea isolate is a useful property for easy manufacture of the preparation for practical stump treatment; the spore production of the nine Latvian isolates varied from 12.8 to 182.8 million spores per Petri dish.…”

Section: Resultsmentioning

confidence: 99%

“…Abundant sporulation of a P. gigantea isolate is a useful property for easy manufacture of the preparation for practical stump treatment; the spore production of the nine Latvian isolates varied from 12.8 to 182.8 million spores per Petri dish. In the material investigated by Sun et al (2009a) the range was from 2.0 to 271.6 million spores per Petri dish.…”

Section: Resultsmentioning

confidence: 99%

“…Investigations by Sun et al (2009aSun et al ( , 2009b showed that efficacy of a P. gigantea isolate against Heterobasidion correlates mostly with its growth rate in wood. The growth rate of Latvian P. gigantea isolates on agar medium at room temperature varied from 5.1 to 8.6 mm day -1 .…”

Section: Resultsmentioning

confidence: 99%

“…Isolates of P. gigantea used in experiments were selected on th rate, spore production, and competitive ability against H. annosum and Investigations by Sun et al (2009aSun et al ( , 2009b showed that efficacy of a correlates mostly with its growth rate in wood. The growth rate of Lat room temperature varied from 5.1 to 8.6 mm day -1 .…”

Section: Resultsmentioning

confidence: 99%

“…Rotstop was included in both experiments. The Latvian isolates had been previously tested in the laboratory for their growth rate on malt extract agar, production of asexual spores (oidia), and antagonistic ability against H. annosum and H. parviporum (Table 1), according to methods used by Sun et al (2009a).…”

Section: Fungal Isolatesmentioning

confidence: 99%

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Biological protection of conifers against Heterobasidion infection – interaction between root-rot fungus and Phlebiopsis igantea

Kenigsvalde¹,

Brauners²,

Zaļuma³

et al. 2017

Research for Rural Development

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The biological control agent Rotstop, composed of spores of Phlebiopsis gigantea, is used for treatment of conifer stumps to reduce the spread of Heterobasidion root rot in commercial forests. Two experiments were conducted to compare the antagonistic ability of the Rotstop isolate and nine Latvian isolates of P. gigantea against root rot fungus Heterobasidion, in wood of Scots pine and Norway spruce. Billets of conifer trees were first sprayed with a spore suspension of P. gigantea and then with Heterobasidion at different concentrations. The presence of fungi in billets was evaluated by morphological characteristics of mycelium. The Latvian isolates of P. gigantea showed similar or even higher values of efficacy against Heterobasidion (average efficacy 84% in spruce and 89% in pine) than the commercially manufactured Finnish preparation Rotstop (61% in spruce and 90% in pine). Latvian isolates of P. gigantea have a potential to be used for preparation of biological control agents in the future.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Fungal Isolatesmentioning

confidence: 99%

See 3 more Smart Citations

Biological protection of conifers against Heterobasidion infection – interaction between root-rot fungus and Phlebiopsis igantea

Kenigsvalde¹,

Brauners²,

Zaļuma³

et al. 2017

Research for Rural Development

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Expression Analysis of the Impact of Culture Filtrates from the Biocontrol Agent, Phlebiopsis gigantea on the Conifer Pathogen, Heterobasidion annosum s.s. Transcriptome

2013

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Phlebiopsis gigantea has been routinely used as the biological control agent for the conifer pathogen Heterobasidion annosum sensu lato, but the actual mechanism for the biocontrol process is not known. To investigate the effect of secreted molecules from culture filtrate produced by P. gigantea on the gene expression profile of H. annosum s.s., microarray analysis was used. Analysis of the differentially expressed genes led to the identification of genes with diverse functions. A major proportion of the up- and downregulated genes were either uncharacterized or genes whose functions were not known. A number of genes coding for proteins involved in metabolism, transport, and signal transduction were differentially downregulated; comparatively lower number of such genes were upregulated. Some genes involved in transport (polyamine transporters, 2573-fold, P = 0.002) and metabolism (endoglucanase, 622.5-fold, P = 0.002, cytochrome P450, 133.2-fold, P = 0.05) showed high transcript fold changes and were statistically significantly upregulated. Genes encoding defense-related proteins such as hydrophobins were either downregulated or expressed at relatively low levels. Further analysis of the effect of the culture filtrate on glucose metabolism showed downregulation of some key enzymes at the early stage of the glycolytic pathway while some genes were upregulated at the later stage of the pathway. A subset of the genes were selected and used to validate the micro-array result by quantitative real time polymerase chain reaction (qPCR) method. Generally, the high transcript levels of genes encoding several biochemically important genes (protein kinases, major facilitator superfamily polyamine transporters, endoglucanase, cytochrome P450, endoglucanase) suggests their potential functional relevance in signal perception, stress tolerance, cell defenses, and detoxification of toxic molecules during competitive interaction. These results have provided further insights into possible molecular and genetic factors underlying the response of H. annosum to metabolites from P. gigantea during interspecific interaction.

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Host-associated Intraspecific Phenotypic Variation in the Saprobic Fungus Phlebiopsis gigantea

et al. 2023

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Whether intraspecific phenotypic variation in saprobic fungi may be driven by the host of origin has received little attention. We addressed this issue by testing hypotheses using the model system Phlebiopsis gigantea, a wood destroying fungus associated with Picea abies and Pinus sylvestris, among others, and widely employed in practical forestry as a biological control agent. By examining approximately 60 sympatric P. gigantea isolates from both P. abies and P. sylvestris, we showed that the former grew in vitro significantly (P < 0.05) slower than the latter (average 5.56 mm/day vs. 6.84) while producing 1.8-fold significantly higher number of mitospores. An overall significant trade-off between these two phenotypic traits was detected, in particular for isolates originating from P. abies. Comparative inoculation experiments of a subsample of isolates and the assessment of mycelial growth in logs of both hosts allowed to reject the hypothesis that isolates are equally fit in terms of growth rate in wood of both hosts regardless of the host of origin. Tree models revealed that the growth rate of isolates was associated not only with the wood species in which the isolates were inoculated (P < 0.001), P. sylvestris being more rapidly colonized than P. abies, but also with the host of origin of isolates (P < 0.001). Results showed that P. gigantea isolates originating from different hosts differ phenotypically in terms of some key phenotypic traits demonstrating that a host-driven intraspecific phenotypic variation may occur in saprobic fungi.

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Variation in properties of Phlebiopsis gigantea related to biocontrol against infection by Heterobasidion spp. in Norway spruce stumps

Cited by 34 publications

References 21 publications

Biological protection of conifers against Heterobasidion infection – interaction between root-rot fungus and Phlebiopsis igantea

Biological protection of conifers against Heterobasidion infection – interaction between root-rot fungus and Phlebiopsis igantea

Expression Analysis of the Impact of Culture Filtrates from the Biocontrol Agent, Phlebiopsis gigantea on the Conifer Pathogen, Heterobasidion annosum s.s. Transcriptome

Host-associated Intraspecific Phenotypic Variation in the Saprobic Fungus Phlebiopsis gigantea

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