Variation in Phenolic Compounds Content and Antioxidant Activity of Different Plant Organs from Rumex crispus L. and Rumex obtusifolius L. at Different Growth Stages

Abstract

The study investigated the accumulation of phenolic compounds and the antioxidant activity of extracts of various parts of R. crispus and R. obtusifolius, collected at the flowering stage and the fruiting stage. Half of the collected plants were divided into root, stem, leaves, and reproductive organs (inflorescence). The other half was used to study the vertical distribution of biologically active components and antioxidants throughout the plant. The samples were analyzed for total catechins content, total proanthocyanidins content, total phenolic content, and total antioxidant activity (1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2’azinobis(3)ethylbenzthiazoline-6-sulfonic acid (ABTS), and ferric reducing antioxidant power (FRAP) assays). All analyses were performed in four replicates. In general, a similar trend was observed in the distribution of phenolic compounds in the studied species. The maximum content of these secondary metabolites was noted in the reproductive organs, both in the flowering and fruiting period. Stems were characterized by a minimum content of the studied classes of substances. The antioxidant activity of the sorrels studied parts can be arranged in the following order: the generative part (flowers, seeds) > leaves > root > stem (for flowering and fruiting stages). It was found that parts of the root closer to the stem differed in higher activity.

Phenylalanine and Tyrosine as Exogenous Precursors of Wheat (Triticum aestivum L.) Secondary Metabolism through PAL-Associated Pathways

“…Plant extract preparation for phenolic compound assays included homogenization of 0.1-0.2 g of plant sample material with 10 mL of 96% ethanol solution and centrifugation of the mixture at 4500× g for 30 min. The supernatant fluid was used for analysis [47].…”

Response Surface Modeling and Optimization of Polyphenols Extraction from Apple Pomace Based on Nonionic Emulsifiers

“…The third indicates the ability of antioxidant to reduce ferric ions. The detailed protocols of analysis were reported previously by authors [31]. Briefly, for the DPPH-assay 30-100 µL of investigated extract was added to 2.85 mL of 0.1 mM DPPH-solution.…”

Dynamic of Phenolic Compounds, Antioxidant Activity, and Yield of Rhubarb under Chemical, Organic and Biological Fertilization

“…Kalisz et al analyzed the polyphenol content of two cultivars (Victoria and Red Malinowy) in the spring and autumn seasons, recording the highest value of flavan-3-ols for Red Malinowy cultivar from spring harvest (195.98 mg•g −1 d.w.) and the lowest for Victoria cv from autumn harvest (86.57 mg•g −1 d.w.) [13]. Studies on other vegetable species belonging to the family Polygonaceae were performed on Rumex acetosa [19][20][21][22], Rumex scutatus [23], Rumex crispus [24,25], Rumex japonicus [26], Rumex hastatus [27][28][29], Rumex ecklonianus [30], Rumex tingitanus [31], Rumex sanguineus [32], Rumex acetosella [33], Rumex maderensis [34], and Rumex obtusifolius [35], but no determinations relevant to polyphenols content and composition were performed.…”