Variation in PAH inputs and microbial community in surface sediments of Hamilton Harbour: Implications to remediation and monitoring

Slater, G. F.; Cowie, B.; Harper, N.S.; Droppo, Ian G.

doi:10.1016/j.envpol.2007.08.009

Cited by 22 publications

(16 citation statements)

References 26 publications

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“…Our results showed that the methane oxidation rate decreased with increasing pyrene concentration. This was consistent with the findings of Slater et al (2008) that methane oxidation activity was weaker in sediments with higher PAH concentration. However, Shannon diversity did not decrease and community structure did not change until the pyrene concentration was high, indicating that methane oxidation rate was more sensitive to pyrene stress than methanotroph community.…”

Section: Discussionsupporting

confidence: 94%

Pyrene effects on methanotroph community and methane oxidation rate, tested by dose–response experiment and resistance and resilience experiment

2010

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Purpose Methanotrophs are an important group of bacteria that can metabolize methane. Polycyclic aromatic hydrocarbons (PAHs) are widespread contaminants and present in all ecosystems. We hypothesize that PAHs may affect methanotrophs and methane oxidation. In this study, we assessed dose-response curves for the inhibition of methane oxidation and methanotrophs diversity by pyrene, and resistance and resilience of soil methane oxidation rate and methanotrophs composition in response to pyrene contamination. Material and methods Methanotrophic bacterial diversity was determined by terminal restriction fragment length polymorphism analysis of the pmoA gene, while methanotrophs composition was assessed by cloning and sequencing of the pmoA gene. Sequences with 98% or more identity were considered as the same operational taxonomic unit (OTU). The pyrene concentration at which methane oxidation decreased by 50%, as compared to the non-spiked control soil (EC 50 ), was determined. Both EC 50 concentration and 500 mgkg −1 of pyrene were applied as disturbances in the resistance and resilience experiment. Resistance and resilience were determined 1 and 40 days, respectively, after spiking pyrene.Results and discussion Methane oxidation rate decreased with increasing pyrene concentrations and the EC 50 value was 22.0 mgkg −1 . Methanotrophic bacterial community diversity decreased in 200 and 500 mgkg −1 pyrene treatments, and methanotroph community structure shifts occurred in 100, 200, and 500 mgkg −1 pyrene treatments. Methane oxidation was neither resistant nor resilient to pyrene disturbance. However, methane oxidation of soil with 22.0 mgkg −1 pyrene disturbance recovered to some extent after 40 days incubation. There were five OTUs identified in the control samples, but the number of OTUs increased 1 day after the addition of 22 mgkg −1 of pyrene. It suggests that a low level of disturbance may increase diversity. Forty days after 500 mgkg −1 of pyrene disturbance, only one OTU belonging to Methylocaldum was detected. The resilience of Methylocaldum to a high level of pyrene could be due to the high genomic GC content, which reduces the frequency of insertion by pyrene into the DNA duplex. In addition, we found that the number of OTUs decreased in all treatments after the 40-day incubation. Conclusions Methane oxidation activity was more sensitive to pyrene than the methanotroph community structure, but could recover under a low level of pyrene. Significant decrease in diversity and shift in species composition occurred only after severe perturbation. A low level of disturbance could increase biodiversity, while a high level of disturbance could decrease it.

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Section: Discussionsupporting

confidence: 94%

Pyrene effects on methanotroph community and methane oxidation rate, tested by dose–response experiment and resistance and resilience experiment

2010

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“…They represent a large and diverse pool of species and exhibit a vast array of metabolic functions. Some of them are key players in biogeochemical processes (Cotner and Biddanda 2002;Falkowski et al 2008), participate in controlling water quality (Fonseca et al 2001;Yu et al 2009) and are responsible for the degradation of organic pollutants released in the environment (Männistö et al 2001;Chinalia and Killham 2006;Slater et al 2008). In the current context of global change, detailed knowledge about the microbial diversity, specific functions and ecology of micro-organisms that inhabit water columns, sediments and microbial biomats is needed (Duffy and Stachowicz 2006).…”

Section: Introductionmentioning

confidence: 99%

Microbial diversity in waters, sediments and microbial mats evaluated using fatty acid-based methods

Mrozik

Nowak

Piotrowska‐Seget

2013

Int. J. Environ. Sci. Technol.

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The review summarises recent advances towards a greater comprehensive assessment of microbial diversity in aquatic environments using the fatty acid methyl esters and phospholipid fatty acids approaches. These methods are commonly used in microbial ecology because they do not require the culturing of micro-organisms, are quantitative and reproducible and provide valuable information regarding the structure of entire microbial communities. Because some fatty acids are associated with taxonomic and functional groups of micro-organisms, they allow particular groups of micro-organisms to be distinguished. The integration of fatty acid-based methods with stable isotopes, RNA and DNA analyses enhances our knowledge of the role of micro-organisms in global nutrient cycles, functional activity and phylogenetic lineages within microbial communities. Additionally, the analysis of fatty acid profiles enables the shifts in the microbial diversity in pristine and contaminated environments to be monitored. The main objective of this review is to present the use of lipid-based approaches for the characterisation of microbial communities in water columns, sediments and biomats.

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“…Although the PAHs levels in Douro 1 sediments were in the mg kg -1 range, all the concentrations measured in the sediments of the 5 selected estuaries/lagoons were below the Effects Range Low value (ERL = 4.02 mg kg -1 ), a guideline value established by the National Oceanographic and Atmospheric Administration (NOAA) (Long and Morgan 1991) corresponding to background concentrations and below which the presence of contaminants has little chronic or acute effect on benthic organisms (Jones et al 1997, Smith et al 2009 Comparatively to the levels obtained by Cachot et al (2006) in Seine estuary, reports from OSPAR (OSPAR 1997, OSPAR 2000 and several studies devoted to Western Europe estuaries (Wadden Sea, Scheldt estuary, Oder estuary, Tampa Bay estuarine system, Pearl river delta (cited in (Cachot et al 2006))) the PAHs levels herein reported can be considered low, originating from background sources and diffuse pollution, except the Lisnave and Douro 1 sites which denoted an increased exposure, probably arising from shipping and port activities. The PAHs levels were lower despite the fact that the OM content was sometimes one order of magnitude higher than in the Seine estuary, which is generally correlated to a higher potential for accumulation of PAHs.…”

Section: A Nalytical Methods Performancementioning

confidence: 99%

“…Sediments are considered a long term sink of such hydrophobic organic micropollutants. PAHs-contaminated sediments are found in many coastal lagoons and estuaries where pollution sources converge and sediments have a long residence time, often under oxygen and microbial deficient conditions (Quantin et al 2005, Slater et al 2008, Smith et al 2009). …”

Section: Introductionmentioning

confidence: 99%

PAHs levels in Portuguese estuaries and lagoons: Salt marsh plants as potential agents for the containment of PAHs contamination in sediments

Gonçalves

Teixeira

Basto

et al. 2016

Regional Studies in Marine Science

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Variation in PAH inputs and microbial community in surface sediments of Hamilton Harbour: Implications to remediation and monitoring

Cited by 22 publications

References 26 publications

Pyrene effects on methanotroph community and methane oxidation rate, tested by dose–response experiment and resistance and resilience experiment

Pyrene effects on methanotroph community and methane oxidation rate, tested by dose–response experiment and resistance and resilience experiment

Microbial diversity in waters, sediments and microbial mats evaluated using fatty acid-based methods

PAHs levels in Portuguese estuaries and lagoons: Salt marsh plants as potential agents for the containment of PAHs contamination in sediments

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