Variation in outer-membrane protein and lipopolysaccharide profiles of Pasteurella haemolytica isolates of serotypes A1 and A2 obtained from pneumonic and healthy cattle

McCluskey, Jackie; Gibbs, H. A.; Davies, Robert L.

doi:10.1099/00221287-140-4-807

Cited by 11 publications

(7 citation statements)

References 16 publications

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“…The isolates belonged to serotypes A1 (49 isolates), A2 (60 isolates), A5 (2 isolates), A6 (21 isolates), A7 (18 isolates), A8 (2 isolates), A9 (7 isolates), A11 (7 isolates), A12 (6 isolates), A13 (3 isolates), A14 (1 isolate), and A16 (1 isolate), and to untypeable groups (see reference 18) UG1 (6 isolates), UG2 (2 isolates), and UG3 (9 isolates). This collection has been the subject of previous investigations (15,18,32). The properties of the isolates that are of direct relevance to the present study, except for those of serotype A11 and untypeable isolates (see references 15 and 18), including electrophoretic type (ET), capsular serotype, LPS type, and OMP type, are presented in Table 1.…”

Section: Methodsmentioning

confidence: 99%

Evolutionary genetics of Pasteurella haemolytica isolates recovered from cattle and sheep

1997

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Genetic diversity and relationships among 194 Pasteurella haemolytica isolates, which were recovered predominantly from cattle (39%) and sheep (58%) suffering from pneumonic pasteurellosis in the United Kingdom, Germany, and the United States, were estimated by examination of allelic variation at 18 enzymeencoding loci detected by multilocus enzyme electrophoresis. The isolates formed two major divisions. One included 178 Pasteurella haemolytica sensu stricto strains representing serotypes A1, A2, A5 to A9, A12 to A14, and A16; the other was composed of 16 isolates belonging to the A11 taxon. P. haemolytica isolates were classified into 22 electrophoretic types (ETs) that formed three primary phylogenetic lineages. One lineage was represented by ovine serotype A2 isolates, a second lineage consisted of bovine serotype A2, together with serotype A7 and A13 isolates, and the third lineage included isolates representing all of the other serotypes, as well as a second group of serotype A7 strains. Electrophoretic types were nonrandomly associated with specific capsular serotypes, lipopolysaccharide (LPS) types, outer membrane protein (OMP) types, and host species. Bovine isolates were represented by only three serotypes (A1, A2, and A6) in 5 ETs, whereas ovine isolates were represented by all of the serotypes in 19 ETs. The majority (76%) of bovine isolates were of serotypes A1 or A6 and belonged to a single ET that marked a virulent, cattle-specific clonal group. Among the ovine isolates, 40% were of serotype A2 and belonged to two ETs that represented two virulent, sheep-specific clonal groups. Bovine A1 and A6 isolates and bovine A2 isolates were phylogenetically distinct from ovine isolates of the same serotypes, indicating that different subpopulations of these serotypes are associated with disease in cattle and sheep. Consistent differences in the OMP profiles of strains of the bovine and ovine lineages of these three serotypes suggest that certain OMPs are involved in host specificity and virulence. Evolutionary relationships among P. haemolytica isolates indicate that the ancestral host is the sheep and that several distinct clonal lineages have crossed the species barrier into cattle. The A11 taxon is a heterogeneous group of opportunistic pathogens of sheep that represents a separate species. Pasteurella haemolytica is the etiological agent of pneumonic pasteurellosis of cattle and sheep, which are infections that cause considerable economic loss to the cattle and sheep industries (19, 24). P. haemolytica consists of 13 capsular serotypes, designated A1, A2, A5 to A9, A11 to A14, A16, and A17 (4, 55). In addition to isolates that express these serotypes, approximately 10% of disease isolates recovered from cattle and sheep are untypeable (21, 45). Whereas some untypeable isolates are closely related to serotype A1, A2, or A11 isolates and probably represent either new capsular types or capsuledeficient strains, others represent distinct species (18, 39, 40). Serotype A1 isolates are responsible for most cas...

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Section: Methodsmentioning

confidence: 99%

Evolutionary genetics of Pasteurella haemolytica isolates recovered from cattle and sheep

1997

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“…LPS gels were run as described above and Western blotting performed as described previously (Davies et al, 1994). The primary antiserum, used at a concentration of 1 : 100, was raised in a calf against live cells of a serotype A1 isolate possessing smooth LPS of type 1A (Ali e t al., 1992).…”

Section: Sds-pagementioning

confidence: 99%

Intra-specific diversity and host specificity within Pasteurella haemolytica based on variation of capsular polysaccharide, lipopolysaccharide and outer-membrane proteins

Davies

Donachie²

1996

Microbiology

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Intra-specific diversity within Pasteurella haemolytica was assessed by analysing variation in the capsular polysaccharide (serotypes), lipopolysaccharide (LPS) and outer-membrane proteins (OMPs) of 184 isolates recovered from cattle and sheep. Four of 12 serotypes comprised 83% of the total number of isolates, including A1 and A2 as the most frequently recovered serotypes from cattle and sheep, respectively. Nine distinct LPS profiles were identified. Four different core-ol igosaccharide patterns were present, each of which occurred alone as rough LPS and also in association with a single 0-antigen profile as smooth LPS; the ninth LPS type was also smooth but had a different O-antigen profile. The capsular serotypes could be divided into four groups based on the dominant LPS profile within each serotype: (1) Al, A6, A9, A12 and A5; (2) A2, A8, A14 and A16; (3) A7 and A13; and (4) A l l . Smooth LPS of type IA, which was found only in the first group, was associated primarily with bovine disease isolates, whereas rough LPS of types 1B and 3B were associated primarily with the group 2 serotypes and ovine disease isolates. Similarly, the variation of OMP profiles generated three groups: (1) Al, A6, A9, A12, A5 and A8; (2) A2, A14 and A16; and (3) A7, A l l and A13. Isolates belonging to groups 2 and 3 exhibited greater diversity in their OMP profiles than those belonging to group 1. Although the majority of group 3 isolates possessed profiles unique to that group, a smaller number of A7 isolates possessed profiles with similarities to those of serotypes A1 or A2. OMP profiles clearly differentiated bovine from ovine isolates of the same serotypes. The association both of specific LPS and OMP profiles with bovine or ovine disease isolates suggested a correlation between specific cell-surface structures and host specificity. The combined analysis of capsular serotypes, LPS types and OMP profiles identified seven major groups within P. haemolytica which were responsible for 59% of the disease cases, suggesting a clonal structure for this species. Overall, comparison of the capsular serotypes, LPS types and OMP profiles proved extremely useful for assessing diversity within P. haemolytica.

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“… a Outer membrane protein (OMP) and lipopolysaccharide (LPS) types have been published previously [ 53 ]. …”

Section: Resultsmentioning

confidence: 99%

Evolution of the leukotoxin promoter in genus Mannheimia

et al. 2009

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Background The Mannheimia species encompass a wide variety of bacterial lifestyles, including opportunistic pathogens and commensals of the ruminant respiratory tract, commensals of the ovine rumen, and pathogens of the ruminant integument. Here we present a scenario for the evolution of the leukotoxin promoter among representatives of the five species within genus Mannheimia . We also consider how the evolution of the leukotoxin operon fits with the evolution and maintenance of virulence. Results The alignment of the intergenic regions upstream of the leukotoxin genes showed significant sequence and positional conservation over a 225-bp stretch immediately proximal to the transcriptional start site of the lktC gene among all Mannheimia strains. However, in the course of the Mannheimia genome evolution, the acquisition of individual noncoding regions upstream of the conserved promoter region has occurred. The rate of evolution estimated branch by branch suggests that the conserved promoter may be affected to different extents by the types of natural selection that potentially operate in regulatory regions. Tandem repeats upstream of the core promoter were confined to M. haemolytica with a strong association between the sequence of the repeat units, the number of repeat units per promoter, and the phylogenetic history of this species. Conclusion The mode of evolution of the intergenic regions upstream of the leukotoxin genes appears to be highly dependent on the lifestyle of the bacterium. Transition from avirulence to virulence has occurred at least once in M. haemolytica with some evolutionary success of bovine serotype A1/A6 strains. Our analysis suggests that changes in cis -regulatory systems have contributed to the derived virulence phenotype by allowing phase-variable expression of the leukotoxin protein. We propose models for how phase shifting and the associated virulence could facilitate transmission to the nasopharynx of new hosts.

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Variation in outer-membrane protein and lipopolysaccharide profiles of Pasteurella haemolytica isolates of serotypes A1 and A2 obtained from pneumonic and healthy cattle

Cited by 11 publications

References 16 publications

Evolutionary genetics of Pasteurella haemolytica isolates recovered from cattle and sheep

Evolutionary genetics of Pasteurella haemolytica isolates recovered from cattle and sheep

Intra-specific diversity and host specificity within Pasteurella haemolytica based on variation of capsular polysaccharide, lipopolysaccharide and outer-membrane proteins

Evolution of the leukotoxin promoter in genus Mannheimia

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