Variation in oral susceptibility to dengue type 2 virus of populations of Aedes aegypti from the islands of Tahiti and Moorea, French Polynesia.

Vazeille-Falcoz, M; Mousson, Laurence; Rodhain, F; Chungue, Eliane; Failloux, Anna‐Bella

doi:10.4269/ajtmh.1999.60.292

Cited by 118 publications

(128 citation statements)

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“…Assay A -Infection assays were performed with sevenday-old females that were allowed to feed for 15 min through a chicken skin membrane covering the base of a glass feeder containing the blood-virus mixture, which was maintained at 37°C. The infectious meal was composed of a virus suspension diluted (1:3) in washed rabbit erythrocytes isolated from arterial blood collected 24 h before the infectious meal (Vazeille-Falcoz et al 1999). To enhance the oviposition rate, bovine serum albumin (BSA) was added to the meal, i.e., red cells were washed in PBS then mixed with L-15 medium complemented with 10% BSA.…”

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confidence: 99%

Failure to demonstrate experimental vertical transmission of the epidemic strain of Chikungunya virus in Aedes albopictus from La Réunion Island, Indian Ocean

Vazeille

Mousson

Failloux

2009

Mem. Inst. Oswaldo Cruz

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Aedes albopictus was responsible for transmission in the first outbreak of chikungunya (CHIK) on La Réunion Island, Indian Ocean, in 2005-2006. The magnitude of the outbreak on this island, which had been free of arboviral diseases for over 30 years, as well as the efficiency of Ae. albopictus as the main vector, raises questions about the maintenance of the CHIK virus (CHIKV) through vertical transmission mechanisms. Few specimens collected from the field as larvae were found to be infected. In this study, Ae. albopictus originating from La Réunion were orally infected with a blood-meal containing 10 8 pfu/mL of the CHIKV epidemic strain (CHIKV 06.21 Chikungunya virus (CHIKV) is a vector-borne alphavirus transmitted to humans by mosquitoes belonging to the genus Aedes. It was first isolated in 1953 in Tanzania (Ross 1956) and until recently outbreaks had only been observed in Africa, Asia and India. In Africa, CHIKV is maintained within a sylvatic cycle with wild mosquitoes as vectors (Jupp & Kemp 1996, Diallo et al. 1999. In Asia and India, a sylvatic cycle has never been demonstrated and CHIKV is mainly transmitted within an urban cycle primarily involving the human-biting Aedes aegypti and the less anthropophilic Aedes albopictus (Jupp & McIntosh 1988 (Salvan & Mouchet 1994). Interestingly, the sequencing of viral strains from La Réunion demonstrated the emergence of a mutation in the course of the outbreak and the mutation was detected in the majority of the viral population (Schuffenecker et al. 2006). This mutation was shown to be associated with a better transmission rate by Ae. albopictus (Tsetdatkin et al. 2007, Vazeille et al. 2007). Furthermore, viral particles could be detected in the salivary glands at day 2 post-infection and high densities of viral particles were observed in eggs at day 6 post-infection. This last observation raised con--infection. This last observation raised concerns of possible maintenance of the virus on the island through vertical transmission, a mechanism by which arboviruses can be maintained in nature when climate conditions are unfavourable to the activity of adults: infected females die but the virus can survive in their desiccated eggs. The frequency of this phenomenon is extremely variable depending on the mosquito species and the virus. Vertical transmission of CHIKV has not yet been demonstrated in the laboratory (Jupp et al. 1981, Mourya 1987, but has been proven to occur in the field in Ae. albopictus, in La Réunion (Delatte et al. 2008) and in Madagascar (Ratsitorahina et al. 2008). However, the transmission rate was low and only viral RNA was detected, with no virus isolated. Therefore, we attempted to demonstrate vertical transmission under laboratory conditions with Ae. albopictus from La Réunion and the epidemic viral strain in order to assess the potential risk of maintenance of CHIKV on the island. MATERIALS AND METHODS Mosquitoes 633± 1°C with 80% relative humidity and a 16:8 h photoperiod. Adults were given 10% sucrose solution and females fed on a mouse...

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Failure to demonstrate experimental vertical transmission of the epidemic strain of Chikungunya virus in Aedes albopictus from La Réunion Island, Indian Ocean

Vazeille

Mousson

Failloux

2009

Mem. Inst. Oswaldo Cruz

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“…In all essays, the Paea strain of Ae. aegypti collected in Tahiti (Vazeille-Falcoz et al 1999) was used as control.…”

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“…Field collected mosquitoes (F 0 ) raised to adult in the laboratory were morphologically identified to species and fed on guinea pigs to obtain eggs. The procedures to maintain and infect mosquitoes followed those described by Vazeille-Falcoz et al (1999).…”

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confidence: 99%

“…Mosquito oral infections were performed with a viral stock corresponding to the supernatant fluid of the 4th pas-sage of this virus strain in C6/36 cells. Viral stock production was done according to Vazeille-Falcoz et al (1999) and titers calculated by the 50% endpoint method described by Reed and Muench (1938) and expressed as 50% mosquito infectious doses (MID 50 )/ml.…”

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confidence: 99%

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Oral Susceptibility to Yellow Fever Virus of Aedes aegypti from Brazil

Lourenço‐de‐Oliveira¹,

Vazeille

Filippis

et al. 2002

Mem. Inst. Oswaldo Cruz

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“…aegypti is also a vector of Wuchereria bancrofti and Brugia malayi, both of which cause lymphatic filariasis or elephantiasis (Service, 2012; Consequently, Ae. aegypti has been the subject of numerous vector competence and population genetic studies (Aitken, Downs and Shope, 1977;Gubler et al, 1979;Tabachnick and Powell, 1979;Rosen et al, 1985;Tabachnick et al, 1985;Tardieux et al, 1990;Miller and Mitchell, 1991;Apostol, Reiter and Miller, 1996;Bosio and Beaty, 1998;Vazeille-Falcoz et al, 1999;Bosio, Fulton and Salasek, 2000;Bennett et al, 2002b;Gorrochotegui-Escalante et al, 2002;Mercado-Curiel, Black and Muñoz, 2008;Lozano-Fuentes et al, 2009;Sylla et al, 2009;Lambrechts, 2011;Lambrechts et al, 2011;Guo et al, 2013;Muñoz et al, 2013b;Chepkorir et al, 2014;Diagne et al, 2014;Dickson et al, 2014;Gonçalves et al, 2014;Vega-Rúa et al, 2014).…”

Section: Genetics Of Vector Competence In Ae Aegypti Vector Competenmentioning

confidence: 99%

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2018

Safety Assessment of Transgenic Organisms in the Environment, Volume 8

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This work is published under the responsibility of the Secretary-General of the OECD. The opinions expressed and arguments employed herein do not necessarily reflect the official views of OECD member countries.This document, as well as any data and any map included herein, are without prejudice to the status of or sovereignty over any territory, to the delimitation of international frontiers and boundaries and to the name of any territory, city or area.Please cite this publication as: OECD (2018) The statistical data for Israel are supplied by and under the responsibility of the relevant Israeli authorities. The use of such data by the OECD is without prejudice to the status of the Golan Heights, East Jerusalem and Israeli settlements in the West Bank under the terms of international law.Photo credits: Cover © Maks Ershov.Corrigenda to OECD publications may be found on line at: www.oecd.org/about/publishing/corrigenda.htm. © OECD 2018You can copy, download or print OECD content for your own use, and you can include excerpts from OECD publications, databases and multimedia products in your own documents, presentations, blogs, websites and teaching materials, provided that suitable acknowledgment of the source and copyright owner(s) is given. All requests for public or commercial use and translation rights should be submitted to rights@oecd.org. Requests for permission to photocopy portions of this material for public or commercial use shall be addressed directly to the Copyright Clearance Center (CCC) at info@copyright.com or the Centre francais d'exploitation du droit de copie (CFC) at contact@cfcopies.com. FOREWORD │ 3SAFETY ASSESSMENTOF TRANSGENIC ORGANISMSIN THE ENVIRONMENT, VOLUME 8 © OECD 2018 ForewordModern biotechnologies are applied to plants species (crops, flowers, trees), animals and micro-organisms. The safety of the resulting transgenic organisms when released in the environment for their use in agriculture, forestry, the food and feed industry or for other applications represents a challenging issue. Genetically engineered products are rigorously assessed by their developers during their elaboration, and by governments when ready for release, to ensure high safety standards. This remains essential with new biotechnology developments using insects to fight against disease outbreaks: engineered mosquitoes need to be evaluated through a scientifically sound approach to risk/safety assessment that will inform biosafety regulators and support the decision concerning the release of these novel organisms in the environment.The OECD offers a long-standing recognised expertise in biosafety and contributes to facilitating an harmonised approach. The OECD's Working Group on Harmonisation of Regulatory Oversight in Biotechnology (WG-HROB) was established in 1995. The WG-HROB gathers national authorities responsible for the environmental risk/safety assessment of products of modern biotechnology in OECD countries and other economies. International organisations and experts involved in biosafety activities are a...

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Variation in oral susceptibility to dengue type 2 virus of populations of Aedes aegypti from the islands of Tahiti and Moorea, French Polynesia.

Cited by 118 publications

References 17 publications

Failure to demonstrate experimental vertical transmission of the epidemic strain of Chikungunya virus in Aedes albopictus from La Réunion Island, Indian Ocean

Failure to demonstrate experimental vertical transmission of the epidemic strain of Chikungunya virus in Aedes albopictus from La Réunion Island, Indian Ocean

Oral Susceptibility to Yellow Fever Virus of Aedes aegypti from Brazil

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