Variation in mutations providing resistance to acetohydroxyacid synthase inhibitors in Cyperus difformis in China

Li, Zheng; Li, Xiangju; Chen, Jingchao; PENG, Li-cun; Wang, Jingjing; Cui, Hailan

doi:10.1016/j.pestbp.2020.104571

Cited by 15 publications

(14 citation statements)

References 43 publications

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“…Our experiments demonstrated that C. difformis populations in China had different resistance levels to bensulfuron-methyl and cross-resistance to multiple ALS inhibitors. Similar results were also reported in C. difformis and the sedge species bog bulrush [Schoenoplectus mucronatus (L.) Palla], annual sedge (Cyperus compressus L.), and rice flatsedge (Cyperus iria L.) with different target-site mutations (Li et al 2020;McCullough et al 2016;Riar et al 2015;Yamato et al 2013). Additionally, all populations died when field-recommended doses of other herbicides with non-ALS inhibiting chemistries, such as MCPA, bentazone, and propanil, were applied.…”

Section: Susceptibility To Multiple Herbicidessupporting

confidence: 81%

“…It has been reported that eight ALS gene mutations conferring resistance to ALS-inhibiting herbicides have been identified in resistant plants (Murphy and Tranel 2019). The target-site mutation at codon Pro-197 could have substitutions of Ala, Ser, Arg, and His, which confer cross-resistance to different ALS inhibitors in C. difformis (Li et al 2020). In this study, the populations with Pro-197-Ser were crossresistant to penoxsulam, imazapic, and bispyribac-sodium.…”

Section: Sequencing and Analysis Of Als Genementioning

confidence: 66%

“…In recent years, the resistance of weeds to ALS-inhibiting herbicides has also been reported in a few paddy fields of Hunan Province. (Li et al 2020;Liu et al 2019a;Peng et al 2019). In fact, resistant C. difformis populations have been one of the most troublesome weed problems in Hunan Province.…”

Section: Herbicide Response Experiments Of Bensulfuron-methylmentioning

confidence: 99%

“…Consequently, due to overreliance on herbicides, many weed species in rice fields have evolved resistance to common herbicides such as penoxsulam, quinclorac, cyhalofop-butyl, bensulfuronmethyl, and propanil (Liu et al 2019b;Pedroso et al 2016;Peng et al 2019;Wei et al 2019;Yang et al 2021;Zhao et al 2017). In particular, C. difformis populations have evolved resistance to ALS-inhibiting herbicides such as bensulfuron-methyl, pyrazosulfuron-ethyl, halosulfuron, penoxsulam, and bispyribac-sodium (Kuk et al 2004;Li et al 2020;Loddo et al 2018;Tehranchian et al 2015b).…”

Section: Introductionmentioning

confidence: 99%

“…Amino acid substitutions by His, Ser, and Ala at position Pro-197 have been reported to confer resistance to ALS-inhibiting herbicides in C. difformis populations (Ntoanidou et al 2016;Tehranchian et al 2015b). Recently, Pro-197-Ser/Arg/Leu, Asp-376-Glu, and Trp-574-Leu mutations have been reported in resistant C. difformis populations from China (Li et al 2020).…”

Section: Introductionmentioning

confidence: 99%

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Comparative analysis of resistance to ALS-inhibiting herbicides in smallflower umbrella sedge (Cyperus difformis) populations from direct-seeded and puddled-transplanted rice systems

et al. 2022

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The transition from puddled-transplanted rice (Oryza sativa L.) (PTR) to direct-seeded rice (DSR) system is gaining popularity in central China. In contrast, the PTR system is the most common practice in southwest China. Weeds are a major problem in the paddy fields of the DSR systems, and herbicides are widely used for weed control. However, the increased frequency and rate of herbicide use leads to the rapid evolution of resistance. Smallflower umbrella sedge (Cyperus difformis L.) is a troublesome weed species in rice fields of China and is usually controlled by the acetolactate synthase (ALS) -inhibiting herbicide bensulfuron-methyl. Here, we collected 32 C. difformis populations from DSR systems (Hunan Province) and PTR systems (Guangxi Province) and investigated their resistance to bensulfuron-methyl. Results revealed 80% (8 out of 10) populations from Hunan Province and 14% (3 out of 22) populations from Guangxi Province had evolved resistance to bensulfuron-methyl. Five populations of Hunan Province (HN-2, HN-3, HN-5, HN-9, HN-10) possessing the Trp-574-Leu had high-level resistance (ranged from 169 to >1309-fold) based on GR50 ratios. The resistant populations from Guangxi Province were at a lower resistance level to bensulfuron-methyl due to Pro-197-Ser mutation. The Asp-376-Glu mutation was only identified in the HN-4 population. In addition, the population GX-3 from the PTR systems was resistant to bensulfuron-methyl without ALS gene mutations, indicating non-target site resistance (NTSR). Although some resistant populations of both regions exhibited cross-resistance to multiple ALS-inhibiting herbicides, including pyrazosulfuron-ethyl, bispyribac-sodium, penoxsulam, and imazapic, sensitivity was also detected to the auxin herbicide MCPA and photosystem II (PSII)-inhibiting herbicides bentazone and propanil. These results indicate that cultivation practices affect resistance evolution in C. difformis. DSR systems exert high selection pressure by selecting the Trp-574-Leu mutation resulting in high-level resistance. In contrast, mutation at Pro197 plus NTSR likely plays a significant role in ALS resistance in the PTR systems.

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Section: Susceptibility To Multiple Herbicidessupporting

confidence: 81%

Section: Sequencing and Analysis Of Als Genementioning

confidence: 66%

Section: Herbicide Response Experiments Of Bensulfuron-methylmentioning

confidence: 99%