Variation in expression of sex factor genes in the Proteus-Providencia group relative to Escherichia coli

Baumberg, Simon; Dennison, Sheena

doi:10.1128/jb.123.1.278-286.1975

Cited by 11 publications

(2 citation statements)

References 40 publications

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“…By both in vitro and in vivo criteria, the PvuII genes are expressed well in E. coli. This is not particularly surprising, as several E. coli genes have been found to be expressed in Proteus species at rates of 5 to 100% of the E. coli level (2,9,24,31), and the RMS2 from a genus in the same tribe as Proteus species (PstI, from Providencia stuartii) is expressed well in E. coli (34).…”

Section: Discussionmentioning

confidence: 97%

Cloning of a restriction-modification system from Proteus vulgaris and its use in analyzing a methylase-sensitive phenotype in Escherichia coli

Blumenthal¹,

Gregory²,

Cooperider³

1985

J Bacteriol

128

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A 4.84-kilobase-pair plasmid was isolated from Proteus vulgaris (ATCC 13315) and cloned into the plasmid vector pBR322. Plasmid pBR322 contains substrate sites for the restriction endonucleases PvuI and PvuII. The recombinant plasmids were resistant to in vitro cleavage by PvuII but not PvuI endonuclease and were found to cause production of PvuII endonuclease or methylase activity or both in Escherichia coli HB101. The approximate endonuclease and methylase gene boundaries were determined through subcloning, Bal 31 resection, insertional inactivation, DNA-dependent translation, and partial DNA sequencing. The two genes are adjacent and appear to be divergently transcribed. Most E. coli strains tested were poorly transformed by the recombinant plasmids, and this was shown by subcloning and insertional inactivation to be due to the PvuII methylase gene. At a low frequency, stable methylase-producing transformants of a methylase-sensitive strain were obtained, and efficiently transformed cell mutants were isolated from them.

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Section: Discussionmentioning

confidence: 97%

Cloning of a restriction-modification system from Proteus vulgaris and its use in analyzing a methylase-sensitive phenotype in Escherichia coli

Blumenthal¹,

Gregory²,

Cooperider³

1985

J Bacteriol

128

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“…Expression studies employing these plasmids in backgrounds which lack cat (E. coli and P. mirabilis PM2) are consistent with the notion that the control of cat expression in P. mirabilis PM13 may reside outside the cloned segment. Whereas the absence of highlevel expression of the P. mirabilis cat in E. coli might be related to the general problem of the anomalous expression which has been observed reciprocally between genes in E. coli and P. mirabilis (3,4), there are examples of P. mirabilis genes being expressed at normal levels in E. coli backgrounds (5,16,17). Furthermore, it is more difficult to account for low-level expression of cat in PM2, since the apparent single copy of the chromosomal cat gene in strain PM13 yields resistance to chloramphenicol concentrations as high as 500 ,ug ml-'.…”

Section: Discussionmentioning

confidence: 99%

Resistance to chloramphenicol in Proteus mirabilis by expression of a chromosomal gene for chloramphenicol acetyltransferase

Charles¹,

Harford²,

Brookfield³

et al. 1985

J Bacteriol

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Proteus mirabilis PM13 is a well-characterized chloramphenicol-sensitive isolate which spontaneously gives rise to resistant colonies on solid media containing chloramphenicol (50 ,ug mlP) at a plating efficiency of 10" to 0-5 Such chloramphenicol-resistant colonies exhibit a novel phenotype with respect to chloramphenicol resistance. When a single colony grown on chloramphenicol agar is transferred to liquid medium and grown in the absence of antibiotic for 150 generations, a population of predominantly sensitive celis arises'. This mutation-reversion phenomenon has been observed in other Proteus species and Providencia strains, wherein resistance has been shown to be mediated in each case by the enzyme chloramphenicol acetyltransferase. The cat gene responsible for the phenomenon is chromosomal and can be cloned from P. mirabilis PM13 with DNA prepared from cells grown in the absence or the presence of chloramphenicol. Recombinant plasmids which confer resistance to chloramphenicol carry an 8.5-kilobase PstI fragment irrespective of the source of host DNA. The location of the cat gene within the PstI fragment was determined by Southern blotting with a cat consensus oligonucleotide corresponding to the expected amino acid sequence of the active site region of * Corresponding author. t Present address: Glaxo, Greenford, Middlesex, United Kingdom.

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Transcription of Escherichia coli ribosomal DNA in Proteus mirabilis

Morgan

Kaplan

1976

Molec. Gen. Genet.

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Transcription of Escherichia coli ribosomal DNA introduced into Proteus mirabilis on F14 is described. We have developed an assay for E. coli coded ribosomal RNA involving fingerprinting of ribonuclease T1 digests of RNA isolated from ribosomal subunits. Sequence differences in the ribosomal RNA of the two species have allowed us to detect E. coli coded 16S, 23S, and 5S ribosomal RNA in ribosomal subunits of the E. coli-P. mirabilis hybrid. The proportion of E. coli coded rRNA in the hybrid is found at a level which is compatible with the number of E. coli (and P. mirabilis) ribosomal DNA sequences. The resulting ribosomal RNA appears in ribosomes in a form which indicates extensive compatibility of E. coli coded ribosomal RNA with P. mirabilis ribosomal proteins and maturational factors.

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Variation in expression of sex factor genes in the Proteus-Providencia group relative to Escherichia coli

Abstract: Several instances of anomalous expression of genes introduced from Escherichia coli K-12 into Proteus mirabilis have been described. It is shown here that control of sex pilus synthesis directed by the F-like R factor Ri and its

Cited by 11 publications

References 40 publications

Cloning of a restriction-modification system from Proteus vulgaris and its use in analyzing a methylase-sensitive phenotype in Escherichia coli

Cloning of a restriction-modification system from Proteus vulgaris and its use in analyzing a methylase-sensitive phenotype in Escherichia coli

Resistance to chloramphenicol in Proteus mirabilis by expression of a chromosomal gene for chloramphenicol acetyltransferase

Transcription of Escherichia coli ribosomal DNA in Proteus mirabilis

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