Variation in E- and Z-guggulsterones of Commiphora wightii

Soni, V. K.; Swarnkar, P. L.; Tyagi, Vikas C.; Pareek, L. K.

doi:10.1016/j.sajb.2009.10.004

Cited by 12 publications

(12 citation statements)

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“…These outcomes depicted that wild mature guggul plants (more than eight years of age) growing in adverse conditions or in rocky tracts had more guggulsterone content. Variation in guggulsterone content among accessions has also been reported by Kulhari et al (2013); Soni et al (2010) and Yadav et al (1999). Soni et al (2010) attributed the discrepancy in guggulsterone content to environmental factors like temperature and rainfall of the concerned geographical region; higher guggulsterone content was obtained during summer (May-July, highest in May) which gradually decreased in the rainy season (Aug-Oct) and was lowest in winter (Nov-March).…”

Section: Resultssupporting

confidence: 67%

“…Variation in guggulsterone content among accessions has also been reported by Kulhari et al (2013); Soni et al (2010) and Yadav et al (1999). Soni et al (2010) attributed the discrepancy in guggulsterone content to environmental factors like temperature and rainfall of the concerned geographical region; higher guggulsterone content was obtained during summer (May-July, highest in May) which gradually decreased in the rainy season (Aug-Oct) and was lowest in winter (Nov-March). In case of geographical locations northern, western and central part of Rajasthan showed maximum amount of guggulsterone whereas southern part of the state produced lower amount.…”

Section: Resultssupporting

confidence: 67%

“…Total guggulsterone yield was variable in different regions of same agroclimatic provinces (hyper-arid) viz. 2.291 w/w in Churu; 2.088 w/w in Bikaner and 1.871 w/w in Jaiselmer (Soni et al 2010). Agrawal et al (2004a) also reported that the content of guggulsterone produced in winters was very limited due to dormant phase of the plant while its production enhanced in summers.…”

Section: Resultsmentioning

confidence: 98%

“…No reports are available regarding quantitative estimation of guggulsterone isomers in wild collections of C. wightii using HPLC. However, the technique has been used for estimation of E and Z isomers of guggulsterone in gum resin exudates of C. wightii and C. mukul, and dietary supplements containing C. mukul guggulipids (Soni et al 2010;Nagarajan et al 2001;Musharraf et al 2011). Preliminary results indicated that negligible amount of guggulsterone isomers were detectable in the leaf and root samples of C. wightii, therefore, only the stem samples showing presence of substantial quantity of guggulsterone were taken up for further studies.…”

Section: Resultsmentioning

confidence: 99%

“…Mesorb et al (1998) validated gradient HPLC method for quantification of E and Z stereoisomers in oleo-gum resin exudates of C. mukul Engl. Soni et al (2010) estimated the content of guggulsterone isomers in resin using HPLC while Dass and Ramawat (2009) used reverse phase column HPLC to determine guggulsterone content in cell and callus cultures of C. wightii. Verma et al (1998) and Singh et al (1995) quantified the two isomeric forms of guggulsterone, simultaneously by HPLC in rat serum after administration of a single dose (50 mg/kg).…”

Section: Introductionmentioning

confidence: 99%

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Quantitative determination of guggulsterone in existing natural populations of Commiphora wightii (Arn.) Bhandari for identification of germplasm having higher guggulsterone content

Kulhari

Sheorayan²,

Chaudhury

et al. 2014

Physiol Mol Biol Plants

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Guggulsterone is an aromatic steroidal ketonic compound obtained from vertical rein ducts and canals of bark of Commiphora wightii (Arn.) Bhandari (Family - Burseraceae). Owing to its multifarious medicinal and therapeutic values as well as its various other significant bioactivities, guggulsterone has high demand in pharmaceutical, perfumery and incense industries. More and more pharmaceutical and perfumery industries are showing interest in guggulsterone, therefore, there is a need for its quantitative determination in existing natural populations of C. wightii. Identification of elite germplasm having higher guggulsterone content can be multiplied through conventional or biotechnological means. In the present study an effort was made to estimate two isoforms of guggulsterone i.e. E and Z guggulsterone in raw exudates of 75 accessions of C. wightii collected from three states of North-western India viz. Rajasthan (19 districts), Haryana (4 districts) and Gujarat (3 districts). Extracted steroid rich fraction from stem samples was fractionated using reverse-phase preparative High Performance Liquid Chromatography (HPLC) coupled with UV/VIS detector operating at wavelength of 250 nm. HPLC analysis of stem samples of wild as well as cultivated plants showed that the concentration of E and Z isomers as well as total guggulsterone was highest in Rajasthan, as compared to Haryana and Gujarat states. Highest concentration of E guggulsterone (487.45 μg/g) and Z guggulsterone (487.68 μg/g) was found in samples collected from Devikot (Jaisalmer) and Palana (Bikaner) respectively, the two hyper-arid regions of Rajasthan, India. Quantitative assay was presented on the basis of calibration curve obtained from a mixture of standard E and Z guggulsterones with different validatory parameters including linearity, selectivity and specificity, accuracy, auto-injector, flow-rate, recoveries, limit of detection and limit of quantification (as per norms of International conference of Hormonization). Present findings revealed the role of environmental factors on biosynthesis of guggulsterone isomers under natural conditions.

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Section: Resultssupporting

confidence: 67%