Variation in ^|^delta;13C and ^|^delta;15N among different tissues of three estuarine bivalves: implications for dietary reconstructions

Yokoyama, Hisashi; Ishihi, Yuka

doi:10.3800/pbr.1.178

Cited by 18 publications

(8 citation statements)

References 28 publications

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“…The 13 C fractionation for the LR WSB of the oyster was 1.0 ‰, which is close to 1.4 ‰ for the LR WSB of R. philippinarum (Yokoyama & Ishihi 2006). On the other hand, there is a large difference in the 15 N fractionation between the LR WSBs of C. gigas (6.0 ‰) and R. philippinarum (3.8 ‰, Yokoyama & Ishihi 2006). The large 15 N fractionation for C. gigas is considered to occur through nitrogen turnover and metabolic processes, which cause the lighter isotopes to be preferentially lost and the heavier ones to be retained (Owens 1987, Fantle et al 1999.…”

Section: Fractionation Valuessupporting

confidence: 64%

“…The observed variation in the 13 C fractionation between the 2 experiments may be due to the difference in the lipid content between the 3 species of bivalves. The 13 C fractionation for the LR WSB of the oyster was 1.0 ‰, which is close to 1.4 ‰ for the LR WSB of R. philippinarum (Yokoyama & Ishihi 2006). On the other hand, there is a large difference in the 15 N fractionation between the LR WSBs of C. gigas (6.0 ‰) and R. philippinarum (3.8 ‰, Yokoyama & Ishihi 2006).…”

Section: Fractionation Valuessupporting

confidence: 49%

See 1 more Smart Citation

Diet–tissue isotopic fractionation of the Pacific oyster Crassostrea gigas

Yokoyama¹,

Ishihi²,

Yamamoto³

2008

Mar. Ecol. Prog. Ser.

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Juveniles of the Pacific oyster Crassostrea gigas were reared on a monospecific microalgal diet in order to quantify their 13 C and 15 N diet-tissue fractionations. The weights of these juveniles increased by up to 18-fold within 33 d. The juveniles reached isotopic equilibria with the diet, enabling calculations of fractionation values. The 13 C fractionation for tissues containing lipids ranked as 0.6 ‰ (gill lamella) > 0.3 ‰ (adductor muscle) >-0.2 ‰ (mantle lobe) >-0.9 ‰ (whole soft body) >-2.2 ‰ (midgut gland), while the 15 N fractionation ranked as 8.7 ‰ (adductor muscle) > 6.5 ‰ (mantle lobe) > 5.4 ‰ (whole soft body) > 5.2 ‰ (gill lamella) > 2.3 ‰ (midgut gland). Removal of lipids shifted the diet-equilibrated δ 13 C and δ 15 N values in all tissues except the adductor muscle, with resultant increases in the 13 C and 15 N fractionation values. The expected annual mean δ 13 C value for the diet of the oyster in the field is-17.0 ‰, which is an intermediate value among the δ 13 C of coastal phytoplankton (-20.2 ‰), epilithon (-20.0 ‰), epipelon (-14.8 ‰), and seaweeds (-14.9 ‰), suggesting that the oyster feeds on a mixture of these micro-and macroalgae. The expected δ 15 N diet value is 3.1 ‰, which is more depleted than values for micro-and macroalgae, suggesting that the 15 N fractionation in the field is smaller than that obtained from the feeding experiment.

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Section: Fractionation Valuessupporting

confidence: 64%

Section: Fractionation Valuessupporting

confidence: 49%

Diet–tissue isotopic fractionation of the Pacific oyster Crassostrea gigas

Yokoyama¹,

Ishihi²,

Yamamoto³

2008

Mar. Ecol. Prog. Ser.

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“…), (Carabel et al . ), (Yokoyama & Ishihi ), (Ng, Wai & Williams ), (Fernandes & Krull ), (Jaschinski, Hansen & Sommer ), (Serrano et al . ), (Kolasinski, Rogers & Frouin ), (Mintenbeck et al .…”

Section: Methodsmentioning

confidence: 99%

Effects of acid treatment on carbon and nitrogen stable isotope ratios in ecological samples: a review and synthesis

2014

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Summary1. Stable isotopes of carbon and nitrogen are mainstay tracers in diverse fields of ecology, particularly in studies of food webs. 2. Investigators are generally interested in tracing dietary C and N, and hence routinely remove non-dietary, inorganic C contained in calcified structures (e.g. shells, bones) by chemical dissolution of the carbonates. Acid treatment can, however, isotopically fractionate samples if part of the organic matter is lost or chemically modified, resulting in potentially altered d 4. Acidification is needed if mechanical removal of calcified structures is unfeasible in carbonate-rich sample matrices containing low organic C and N, but should otherwise be very carefully considered before its use as a routine pre-treatment step of biological samples in isotope ratio mass spectrometry.

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“…Freshwater insects were washed to remove particulate matter using Mili-Q water and kept whole for further analyses. Because short acid treatments had no significant effect on δ 13 C or δ 15 N of muscle tissue (Yokoyama and Ishihi 2006), tissue samples of mollusks and crustaceans were immersed in 1.2 N HCl for 1 min to remove traces of carbonate and then washed with Mili-Q water. All samples were dried at 60°C for 24 h and ground to a fine powder.…”

Section: Stable Isotope Analysismentioning

confidence: 99%

Foraging behavior of yellow-phase Japanese eels between connected fresh- and brackish water habitats

et al. 2020

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Japanese eels (Anguilla japonica) utilize a broad range of habitats along the marine-freshwater ecotone during their growth phase in inland waters. This study aimed to analyze the foraging behavior of yellowphase Japanese eels in connected fresh-and brackish water habitats and to connect foraging behavior and habitat use through the analysis of carbon (δ 13 C) and nitrogen (δ 15 N) stable isotopes. Stomach contents of eels collected in fresh-and brackish waters were analyzed to identify food sources. Values of δ 13 C and δ 15 N were analyzed in eels and their potential food sources, and used to predict the recent foraging patterns of eels in the Matsukawa-ura, a brackish water lagoon, and in three freshwater tributaries. Eels preyed on the benthic river community and the mudflat community of the lagoon. Gobiid fishes were found to be an important food source for eels in fresh-and brackish water habitats, while Japanese mitten crabs (Eriocheir japonica) and shore crabs (Hemigrapsus spp.) were major prey in fresh-and brackish waters respectively. δ 13 C values of potential eel prey differed significantly between freshand brackish waters and were used to classify three recent patterns of foraging by the 73 eels included in this study: 1) freshwater foraging, 2) brackish water foraging, and 3) multiple-habitat foraging. The data suggested that some eels recently or frequently moved between fresh-and brackish water habitats while others demonstrated a higher fidelity to either fresh-or brackish waters. Isotopic characteristics of prey in the respective foraging habitats revealed the plasticity of habitat use of yellow eels in the study area. This study demonstrates an integrated approach to study habitat use and foraging behavior of eels. Furthermore, the study underlines the need to consider freshwater and estuarine habitats and the connectivity between them for eel management and conservation.

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Variation in ^|^delta;13C and ^|^delta;15N among different tissues of three estuarine bivalves: implications for dietary reconstructions

Cited by 18 publications

References 28 publications

Diet–tissue isotopic fractionation of the Pacific oyster Crassostrea gigas

Diet–tissue isotopic fractionation of the Pacific oyster Crassostrea gigas

Effects of acid treatment on carbon and nitrogen stable isotope ratios in ecological samples: a review and synthesis

Foraging behavior of yellow-phase Japanese eels between connected fresh- and brackish water habitats

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