Variation in Content of Taxol and Related Taxanes in Eastern Himalayan Populations of Taxus wallichiana

Mukherjee, Swapna; Ghosh, Biswajit; Jha, Timir Baran; Jha, Sumita

doi:10.1055/s-2002-33808

Cited by 29 publications

(16 citation statements)

References 6 publications

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“…mongholicus (Ma et al, 2002). Seasonal variation in the quantity of the phytochemicals in the plants with anticancer activity like Crinum macowanii, Taxus baccata, Taxus wallichiana and Taxus brevifolia, have also been reported (Wheeler et al, 1992;Vance et al, 1994;Glowniak et al, 1999;Mukherjee et al, 2002;Elgorashi et al, 2002).…”

Section: Discussionmentioning

confidence: 96%

The effect of seasonal variation on the antineoplastic activity of Alstonia scholaris R. Br. in HeLa cells

Jagetia

Baliga

2005

Journal of Ethnopharmacology

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Section: Discussionmentioning

confidence: 96%

The effect of seasonal variation on the antineoplastic activity of Alstonia scholaris R. Br. in HeLa cells

Jagetia

Baliga

2005

Journal of Ethnopharmacology

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“…Increasing demand for Taxol and resource paucity of Pacific yew trees have driven several research groups to improve the production yield. Various strategies have so far been exploited to advance the Taxol production methodologies, including: direct extraction from natural resources (Mukherjee et al, 2002), semi/total-synthetic methods (Nicolaou et al, 1994;Kingston et al, 1998), and biotechnological approaches (Stierle et al, 1993;Goleniowski, 2000;Huang et al, 2001a;Wu and Lin, 2003). Among the biotechnological methods, stimulation of metabolic pathways in the cultivated plant cells toward production of a desired compound is the one most likely to result a dramatic increase in secondary metabolite yield .…”

Section: Discussionmentioning

confidence: 99%

Improved Taxol production by combination of inducing factors in suspension cell culture of Taxus baccata

Khosroushahi

Valizadeh

Ghasempour

et al. 2006

Cell Biology International

131

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To date enormous attempts have been devoted to improve Taxol production exploiting various methodologies from bioprocess engineering to biotechnological and synthetic approaches. We have developed a 2-stage suspension cell culture of Taxus baccata L. using modified B5 medium in order to improve cell growth as well as productivity. After callus induction and cell line selection, B5 medium was supplemented with vanadyl sulfate (0.1 mg/l), silver nitrate (0.3 mg/l) and cobalt chloride (0.25 mg/l) at the first day of stage I culture to maximize cell growth. This medium was further supplemented with sucrose (1%) and ammonium citrate (50 mg/l) on day 10 and sucrose (1%) and phenylalanine (0.1 mM) on day 20 (i.e., biomass growth medium). At stage II (day 25), two different concentrations of several elicitors such as methyl jasmonate (10 or 20 mg/l), salicylic acid (50 or 100 mg/l) and fungal elicitor (25 or 50 mg/l) were added to the biomass growth medium with the aim of improving cellular productivity. For morphological analysis, microscopic inspection was carried out during cultivation. Cell-associated and extracellular amount of Taxol were detected and measured using HPLC methodology. At stage I, overall Taxol amount of biomass growth medium was 13.75 mg/l (i.e., 5.6-fold higher than that of untreated B5 control). At stage II, treated cells with methyl jasmonate (10 mg/l), salicylic acid (100 mg/l) and fungal elicitor (25 mg/l) produced the highest amount of Taxol (39.5 mg/l), which is 16-fold higher than that of untreated B5 control (2.45 mg/l). Microscopic analyses of Taxus cells in suspension cultures showed various positional auto-fluorescence showing direct correlation with Taxol production. Our studies revealed that intervallic supplementation of B5 medium with combination of biomass growth factors at stage I and mixture of elicitors at stage II could significantly increase Taxol production. Thus, we suggest that the exploitation of this methodology may improve the production of Taxol since demands for Taxol pharmaceuticals are increasingly growing and resource paucities have limited its direct harvesting from Taxus trees.

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“…Taxus is economically important as the source of paclitaxel, a cancer-inhibiting compound found in the bark of yew trees (Wani et al 1971;Fang et al 1996;Poupat et al 2000;Mukherjee et al 2002). This has led to an overexploitation of Taxus wallichiana Zucc.…”

Section: Introductionmentioning

confidence: 99%

High variation and strong phylogeographic pattern among cpDNA haplotypes in Taxus wallichiana (Taxaceae) in China and North Vietnam

et al. 2007

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We studied the phylogeography of Chinese yew (Taxus wallichiana), a tree species distributed over most of southern China and adjacent regions. A total of 1235 individuals from 50 populations from China and North Vietnam were analysed for chloroplast DNA variation using polymerase chain reaction-restriction fragment length polymorphism of the trnL-F intron-spacer region. A total of 19 different haplotypes were distinguished. We found a very high level of population differentiation and a strong phylogeographic pattern, suggesting low levels of recurrent gene flow among populations. Haplotype differentiation was most marked along the boundary between the Sino-Himalayan and Sino-Japanese Forest floristic subkingdoms, with only one haplotype being shared among these two subkingdoms. The Malesian and Sino-Himalayan Forest subkingdoms had five and 10 haplotypes, respectively, while the relatively large Sino-Japanese Forest subkingdom had only eight. The strong geography-haplotype correlation persisted at the regional floristic level, with most regions possessing a unique set of haplotypes, except for the central China region. Strong landscape effects were observed in the Hengduan and Dabashan mountains, where steep mountains and valleys might have been natural dispersal barriers. The molecular phylogenetic data, together with the geographic distribution of the haplotypes, suggest the existence of several localized refugia during the last glaciation from which the present-day distribution may be derived. The pattern of haplotype distribution across China and North Vietnam corresponded well with the current taxonomic delineation of the three intraspecific varieties of T. wallichiana.

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Variation in Content of Taxol and Related Taxanes in Eastern Himalayan Populations of Taxus wallichiana

Cited by 29 publications

References 6 publications

The effect of seasonal variation on the antineoplastic activity of Alstonia scholaris R. Br. in HeLa cells

The effect of seasonal variation on the antineoplastic activity of Alstonia scholaris R. Br. in HeLa cells

Improved Taxol production by combination of inducing factors in suspension cell culture of Taxus baccata

High variation and strong phylogeographic pattern among cpDNA haplotypes in Taxus wallichiana (Taxaceae) in China and North Vietnam

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