Variation in Botryosphaeriaceae from Eucalyptus plantations in YunNan Province in southwestern China across a climatic gradient

Abstract

The Botryosphaeriaceae accommodates many important pathogens of woody plants, including Eucalyptus. Recently, Botryosphaeriaceae were isolated from diseased plant parts from surveys of Eucalyptus plantations in the YunNan Province, China. The aims of this study were to identify these Botryosphaeriaceae isolates and to evaluate their pathogenicity to Eucalyptus. A total of 166 isolates of Botryosphaeriaceae were obtained from six regions in the YunNan Province, of which 76 were from Eucalyptus urophylla × E. grandis hybrids, 49 from E. globulus trees, and 41 isolates were from other unknown Eucalyptus species or hybrids. Isolates were identified by comparing DNA sequences of the internal transcribed spacer ribosomal RNA locus (ITS), partial translation elongation factor 1-alpha (tef1), β-tubulin 2 (tub2) and DNA-directed RNA polymerase II subunit (rpb2) genes, and combined with their morphological characteristics. Eleven species were identified, including Botryosphaeria fusispora, B. wangensis, Lasiodiplodia pseudotheobromae, Neofusicoccum kwambonambiense, N. parvum, and six novel species described as B. puerensis, N. dianense, N. magniconidium, N. ningerense, N. parviconidium and N. yunnanense. The dominant species across the regions were N. yunnanense, N. parvum and B. wangensis, representing 31.3, 25.3 and 19.9% of the total isolates, respectively. Species diversity and composition changed across the different climatic zones, despite their relatively close geographic proximity and the fact that some of the species have a global distribution. All the Botryosphaeriaceae species were pathogenic to one-year-old plants of an E. urophylla × E. grandis clone and E. globulus seed-derived plants, but showed significant inter- and intra-species variation in aggressiveness amongst isolates. The study provides a foundation for monitoring and management of Botryosphaeriaceae through selection and breeding of Eucalyptus in the YunNan Province of southwestern China.

Genetic Diversity and Pathogenicity of Botryosphaeriaceae Species Associated with Symptomatic Citrus Plants in Europe

“…in Iran [ 16 ]. Species of Diplodia , Dothiorella , Lasiodiplodia , and Neofusicoccum detected in our study are widely reported as pathogens of other host plants in Algeria and Tunisia [ 39 , 40 ], Australia [ 41 ], Brazil [ 42 ], China [ 43 , 44 ], Chile [ 45 ], Italy, Portugal [ 39 , 46 , 47 , 48 ], South Africa [ 49 ], and the USA [ 13 , 31 , 36 ]. The results obtained in our study provide valuable information related to the richness, occurrence, and pathogenicity of Botryosphaeriaceae species in association with citrus species.…”

Species Diversity, Mating Strategy and Pathogenicity of Calonectria Species from Diseased Leaves and Soils in the Eucalyptus Plantation in Southern China

“…7 "No" represents the relative locus was not successfully amplified in the current study. 8 "-" represents the relative locus was not amplified in the current study. 9 Isolates used for phylogenetic analyses.…”

Botryosphaeriaceae diversity on Eucalyptus clones in different climate zones of Indonesia

“…More than 40 species in the family have been reported on these trees from many different areas of the world (Farr & Rossman, 2019). Australia, South Africa and China are among those countries where extensive studies have been conducted on the Botryosphaeriaceae infecting Eucalyptus (Burgess et al, 2019; Jami et al, 2017; Li et al, 2020). To date, 17 species have been identified mostly from asymptomatic Eucalyptus spp.…”

“…In China, 11 species have been reported from die-back, leaf and shoot blight, stem and branch canker of Eucalyptus spp. (Li et al, 2020).…”