Variation in Antisperm Antibody Results Using Different Assays

Peters, Albert J.; Ivanović, Milica; Jeyendran, Rajasingam S.

doi:10.1111/j.1600-0897.1995.tb01151.x

Cited by 9 publications

(7 citation statements)

References 12 publications

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“…, 1995). The differences noticed are assumed to depend upon the cellular location of the sperm antigens, the type of Ig‐class present, and the pre‐treatment of the patient's sperm sample (Peters et al. , 1995).…”

Section: Discussionmentioning

confidence: 99%

“…ASA in serum are occurring in up to 30% of infertile men (Peters & Coulam, 1992;Heidenreich et al, 1994;Naz & Menge, 1994) and up to 70% of vasectomized men (Liskin et al, 1983). Several laboratory tests for ASA are available, and the correlation of the results both with different tests and the fertility prognosis has been evaluated in various ways (Meinertz & Hjort, 1986;Dondero et al, 1991Dondero et al, , 1997Collins et al, 1993;Andreou et al, 1995;Peters et al, 1995). The differences noticed are assumed to depend upon the cellular location of the sperm antigens, the type of Ig-class 43, 70 50, 55, 62, 90 25, 120, 190 The bands are divided in groups according to their colour intensity.…”

Section: Discussionmentioning

confidence: 99%

“…-, No detectable bands; F, female subjects. present, and the pre-treatment of the patient's sperm sample (Peters et al, 1995). It seems that the mixed antiglobulin reaction (MAR, Meinertz, 1987) and TAT (Friberg, 1980) are the most preferred methods.…”

Section: Discussionmentioning

confidence: 99%

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Prostasome antigens as targets for sperm agglutinating antibodies demonstrated by 1‐D gel electrophoresis and immunoblottings

et al. 2004

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Many immunoinfertile men have sperm agglutinating antibodies that are directed against prostasome-derived antigens, but these antigens have not been defined so far. We selected serum samples with high ELISA titres against prostasomes from a group of immunoinfertile patients with sperm agglutinating antibodies and used the sera for immunoblottings on 1-D SDS-PAGE of prostasomes and sperm cells. The immunoblottings with individual antiprostasome antisera on 1-D SDS-PAGE of prostasomes, revealed three to 10 bands for each serum. Eighty-five per cent of the serum samples contained bands in the 70-75 kDa region and 80% of the samples contained bands in the 50-55 kDa region. Immunoblottings of extracted sperm cells, revealed one to six bands in the molecular weight range 25-82 kDa and two of the samples recognized two bands with molecular weights (50 and 43 kDa) similar to immunoblottings of prostasomes. The prostasomal antigens recognized by the high titre-antisera of immunoinfertile men were generally different from the sperm antigens recognized by the same sera. This suggests that prostasomes offer a new set of antigens available for research on male immunoinfertility and immunocontraception.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Prostasome antigens as targets for sperm agglutinating antibodies demonstrated by 1‐D gel electrophoresis and immunoblottings

et al. 2004

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“…acute lymphoblastic leukaemia, malignant lymphoma and Wilm's tumour) or non malignant diseases (e.g. Since the results of antibody testing by one method do not always agree with those obtained in other tests, the use of two different assays may detect ASA-positive sera with more reliability than single assay testing (Andreou et al, 1995;Peters et al, 1995). The development of autoimmunity against spermatozoa has not previously been reported in children who have undergone either chemotherapy or combined radio-and polychemotherapy for acute lymphoblastic leukaemia, malignant lymphoma, Wilm's tumour or nephrotic syndrome.…”

Section: Discussionmentioning

confidence: 99%

Antisperm antibodies in prepubertal boys treated with chemotherapy for malignant or non‐malignant diseases and in boys with genital tract abnormalities

et al. 1997

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In several childhood diseases which have the ensuing risk of infertility in adult life because of direct hypothalamic-pituitary-testicular axis involvement, or as a consequence of therapeutic toxicity, the role of antisperm antibodies (ASA) is rarely addressed. The aim of this study was to investigate the occurrence of ASA in a large prepubertal male population (aged 1.2-13 years) consisting of three groups: Group I, 52 patients affected by malignant diseases (lymphoblastic leukaemia, malignant lymphoma, or Wilm's tumour, n = 42), or by nephrotic syndrome (n = 10); Group II, 212 patients with either genital tract abnormalities (cryptorchidism, inguinal hernia, funicular torsion or hypospadias, n = 202), or cystic fibrosis (n = 10); Group III: 100 age-matched normal boys. Group I and II patients were investigated at diagnosis and during or after treatment (drug, radiation or surgical therapy). Group III was used as controls. ASA were detected in sera by the Tray Agglutination Test (TAT) and indirect IgG, IgA and IgM immunobead tests (iIBT). All normal boys were ASA-negative using both tests. Twenty-six out of the 264 patients (9.8%) in Groups I and II were ASA-positive: 23 (8.7%) patients had a positive TAT with a titre of 1:32 to 1:128, whilst 14 (5.3%) had IgG-ASA after iIBT. Eleven patients (4.1%) were ASA-positive in both tests. Of the 26 ASA-positive boys, 24 had genital tract abnormalities (cryptorchidism, testicular torsion, hypospadias) and two had leukaemia with testicular infiltration. Treatment did not modify antibody positivity. Our data confirm that ASA can occur in prepubertal boys, mostly among cases with urogenital pathology, but that it is rare among other cases. Therefore autoimmune reaction against spermatozoa is another factor that should be considered in the evaluation of several conditions in childhood involving reproductive tract alteration and potential impairment of the blood testis (Sertoli cell) barrier.

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“…Of 20 trials examining the effect of ASA on fertilization, 14 showed a decrease in the fertilization rate, four showed no effect, and two showed equivocal effects [15]. The differences noticed may result from the ASA isotypes present or the pretreatment of the patient’s sperm samples [16]. In addition, the available tests detect antibody binding to various cellular locations on sperm.…”

Section: Introductionmentioning

confidence: 99%

A Novel Tissue Engineering‐based Assay for Immunological Infertility

et al. 2008

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Antisperm antibodies (ASA) are present in 9–36% of infertile couples, a condition called immunological infertility. The variability of ASA in terms of antigenic specificity and biological effects has made it difficult to design a test able to distinguish reliably between ASA that contribute to infertility and those that do not. To develop a reliable and reproducible method able to detect sperm antibodies, we took advantage of recent progress made in tissue engineering techniques. We used collagen gel as a bio‐scaffold for the production of engineered sperm analogues. The advantages of using collagen gels include biocompatibility, ease of fabrication and low cost. We found that this tissue engineering‐based assay is more specific and more sensitive than a conventional test routinely used for ASA detection. In addition, it exhibited low intra‐ and inter‐variations. We envision the use of this novel approach for the detection of a variety of autoantibodies in autoimmune diseases. In addition to diagnostic purposes, tissue‐engineering based tests could be useful in monitoring treatments with bio‐drugs.

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Variation in Antisperm Antibody Results Using Different Assays

Abstract: These findings suggest that the utilization of different assays to detect ASA may detect sera that are positive for ASA with more reliability than single assay testing.

Cited by 9 publications

References 12 publications

Prostasome antigens as targets for sperm agglutinating antibodies demonstrated by 1‐D gel electrophoresis and immunoblottings

Prostasome antigens as targets for sperm agglutinating antibodies demonstrated by 1‐D gel electrophoresis and immunoblottings

Antisperm antibodies in prepubertal boys treated with chemotherapy for malignant or non‐malignant diseases and in boys with genital tract abnormalities

A Novel Tissue Engineering‐based Assay for Immunological Infertility

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