Variation in Amino Acid Contents in Chickpea Cultivars in Response to Ascochyta rabiei Infection

Abstract

The metabolic changes that took place during host-pathogen interaction have been poorly investigated. Although photosynthesis disturbance and carbon metabolism re-programming have been studied yet, there is scary of data on plant amino acids after induction of resistance. Here we investigated the amino acid contents in induced and un-inoculated and invaded region after induction of resistance. The quantities of methionine, iso-leucine, leucine, tyrosine and phenylalanine contents in three (Pb-91, C-44 and Bittle-98) chickpea cultivars increased after induction and stress of A. rabiei and comparatively higher than induced unstressed plants. The increase was more evident in case of Bion treatment as compared to all other treatments with SA and KOH. Similar trend was shown by plant extracts where in neem extract induced higher amino acid contents. The cultivar C-44 responds efficiently as compared with other two cultivars. The results of the current study showed that amino acids play significant role in resistance and susceptibility of chickpea cultivars against A. rabiei.

Evaluation of Ascochyta resistance in chickpea genotypes with quantitative polymerase chain reaction assay

“…Sahi et al (2012) evaluated 52 lines and indicated that three lines were resistant, 30 moderately resistant while 12 and 3 lines exhibited susceptible and moderately susceptible reactions, respectively. Similar studies on the screening of chickpea materials for resistance to A. rabiei were performed by many researchers (Bokhari et al, 2011;Ghazanfar et al, 2010;Kaur et al, 2013).…”

Relationship Between Induced Resistance and Manganese Zinc and Copper Contents of Susceptible Chickpea Cultivars After Their Inoculation With Ascochyta Rabiei

“…Plant Material: Seeds of three chickpea cultivars viz C-44, Pb-91 and Bittle-98 with susceptible response (Ghazanfar et al,2010a) but good yield character (Ghazanfar et al,2010b) were taken and cultivated in small plots in the experimental area of the Department of Plant Pathology, University of Agriculture, Faisalabad, during the year 2007-08. The experiment was laid out under split-split design with varieties in main plot, doses in subplots and inducers in subsubplots with three replications.…”

“…The disease can be managed by some cultural practices (Ilyas et al, 2007) and use of chemotherapeutants (Singh and Singh, 1990;Tripathi et al, 1987) but the most economical practice is cultivation of cultivars resistant to blight disease (Nene and Reddy, 1987). These control strategies did not last long due to appearance of more virulent strains of the pathogen (Ali et al,2009;Sarwar et al,2000) and scarcity of resistance in the available germplam (Ghazanfar et al,2010a). This necessitates exploring new methods to over come this disease.…”