Variation and intraspecies classification of edible canna (Canna indica L.) based on morphological characters

Sari, Nilam; Purnomo, Purnomo; Daryono, Budi Setiadi; Suryadiantina,; Setyowati, Mamik

doi:10.1063/1.4953515

Cited by 17 publications

(19 citation statements)

References 14 publications

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“…The combination of cluster analysis and principal component analysis has a purpose to result in a fundamental empirical role of morphological characters in grouping accession (Sari et al, 2016). The result of cluster analysis showed two main clusters on the dendrogram (Figure 3) with a similarity coefficient of 0.57 -1.…”

Section: Cluster Ana;ysis and Principal Component Analysismentioning

confidence: 99%

Variability and Intra-Specific Classification of Lima Bean (Phaseolus lunatus L.) from Timor Island based on Morphological Characters

Bria

Suharyanto

Purnomo

2019

J.Tropical Biodiversity Biotechnology

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Lima bean (Phaseolus lunatus L.) is a species of beans which originating from the regions of Central America and Andes Mountains. Lima bean in Timor Island is underutilized although these plant growth there and have many variations. This study aims to determine the diversity of lima beans on Timor Island based on morphological characters. Samples were collected by survey methods from three districts on the island of Timor. Morphological traits related to the vegetative and flowering stages and mature seeds morphology were scored using the International Plant Genetic Resources Institute lima bean descriptors with a soft modification. The similarity index is calculated using the General Similarity Coefficient Gower formula. The dendrogram is generated from cluster analysis using the Unweighted Pair Group Methods using Arithmetic Average (UPGMA) method. Furthermore, Principal Component Analysis (PCA) was used to determine the role of each morphological character used. The dendrogram shows that 23 collected accessions are divided into two main clusters with a 57% similarity index. The two clusters are distinguished based on the presence or absence of secondary colors and secondary patterns in the seed organs. Then, each main cluster is divided into two subclasses based on the character of the pigmentation stem, the length of the terminal leaflets, flower color, and seed type.

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Section: Cluster Ana;ysis and Principal Component Analysismentioning

confidence: 99%

Variability and Intra-Specific Classification of Lima Bean (Phaseolus lunatus L.) from Timor Island based on Morphological Characters

Bria

Suharyanto

Purnomo

2019

J.Tropical Biodiversity Biotechnology

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“…In PCA (Fig. 10) it is not only shown the direction of distribution, but also the influence of the size of the characters that are shown by lines of different lengths (Sari et al, 2016). The longer the arrow line, the greater the influence given in grouping.…”

Section: Resultsmentioning

confidence: 96%

Variations and Phenetic Analysis of Peanut Cultivars (Arachis hypogaea L.) Based on Morphological Characteristics

Purnomo

Khotimah

2019

J.Tropical Biodiversity Biotechnology

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Peanut (Arachis hypogaea L.) is a food commodity that is widely cultivated in Indonesia. At present there has been no analysis of the relationship between peanut cultivars with phenetic methods based on the morphological properties of the plants. Four cultivars of Arachis hypogaea L. Tuban, Talam 1, Talam 2, and Talam 3 used in this research. Morphological characters data was analyzed by description to construct identification key. Similarity index was counted by Simple Matching Coefficient (SSm) formula based on morphological scoring. Cluster analysis was conducted by UPGMA (Unweighted Pair Group Methods using Arithmetic Averages) method to construct dendrogram. PCA (Principal Component Analysis) were performed to defined role of each morphological character in grouping of accessions with MVSP (Multivariate Statistical Program) v. 3.1 software. The dendrogram showed that four cultivars of Arachis hypogaea L. divided into two main clusters, 4 sub-clusters. The similarity index of clusters is 0.85%.

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“…Polymorphism refers to the visualization of amplification based on different observed DNA fragments and score in order to determine whether the variation is presented by observing the sequence differences. RAPD is still used to analyze genetic diversity because of its ability to amplify various species, like Pinus merkusii [19] in Educational Forest Hasanuddin University, onion (Allium cepa L) [20], Dioscorea sp [21], and Indonesian Edible Canna (Canna indica L.) [22]. The comparison co-dominant and dominant primers have been performed in molecular research, in genetic diversity using RAPD and SSR of Ustilago hordei [23], and Zingiber officinale [24].…”

Section: Resultsmentioning

confidence: 99%

Selection of Dominant and Co-dominant Markers for Red Wood (Pterocarpus indicus Willd) Polymorphism from Five Provenances in East Nusa Tenggara

Larekeng¹

2019

Proceedings of the 1st International Conference on Science and Technology, ICOST 2019, 2-3 May, Makassar, Indonesia

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Information about the genetic diversity of Red wood is needed to improve the quality and quantity of the plant. This information can be provided by analyzing plant DNA using molecular markers which are polymorphic and generate clear and thick bands. The objective of the study was to obtain primers that could generate clear and polymorphically bands from Red wood DNA. The evaluated Red wood was from Alor, Sika, Lembata, Rote, and Timur Tengah Selatan (TTS) provenances. As many as 54 primers screened to polymorphism assay. Those primers consisted of RAPD, SSR, and chloroplast.The SSR primers were developed from Pterocarpus indicus (six primers) and Prosopis alba (12 primers). Chloroplast primers were divided into two types, four Rubisco Large Sub-unit genes (rbcL) and 12 MaturaseK genes (matK). SSR primers of P. alba were not able to amplify Red wood DNA, whereas those of P. indicus could amplify the DNA. Three of each chloroplast primers successfully amplified the DNA, yet, each primer could only amplify one to four of 12 evaluated samples. rbcL 2 generated the highest number of sample, and samples from Lembata were amplified the most. Moreover, 16 RAPD primers were able to generate DNA.

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Variation and intraspecies classification of edible canna (Canna indica L.) based on morphological characters

Cited by 17 publications

References 14 publications

Variability and Intra-Specific Classification of Lima Bean (Phaseolus lunatus L.) from Timor Island based on Morphological Characters

Variability and Intra-Specific Classification of Lima Bean (Phaseolus lunatus L.) from Timor Island based on Morphological Characters

Variations and Phenetic Analysis of Peanut Cultivars (Arachis hypogaea L.) Based on Morphological Characteristics

Selection of Dominant and Co-dominant Markers for Red Wood (Pterocarpus indicus Willd) Polymorphism from Five Provenances in East Nusa Tenggara

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