Variasi Biokimia Genetik Populasi Ikan Betutu (Oxyeleotris marmorata, BLKr.) di Waduk Penjalin Brebes

Abstract

Penjalin water reservoir in Brebes Regency, Central Java, is one of the habitats of the sand goby. A study on its genetic diversity using approaches of isozyme analysis was needed to support domestication of the fish in this area. This study was aimed at the biochemical-genetic variation of sand goby population in Penjalin water reservoir based on esterase (EST), peroxidase (PER), malate dehydrogenase (MDH), aspartate amino-transferase (AAT), and acid phosphatase (ACP) polymorphisms. Visualization of the isozymes was carried out employing horizontal electrophoretic technique with potato starch gel and buffer system of L-histidin monohydrate. Of the five isozymes, ACP was not well-visualized in all samples tested while the remaining four showed no polymorphisms. It could be concluded that there is no biochemical-genetic variation of sand goby population in Penjalin water reservoir based on isozymes of EST, PER, MDH, and AAT.

DNA barcoding and morphometric of Rastrelliger spp. in North Maluku Sea, Indonesia

“…Research on fish genetic in Indonesia has been carried out including genetic studies of large-eyed tuna populations (Thunnus obesus) in Benoa Bali (Nugraha, 2009), candra fish (Torsoro) originating from North Sumatra Province and West Java Province (Nugroho et al, 2006), red snapper (Lutjanus malabaricus) originating from several fishing areas of the North Coast of Java and the eastern Java Sea (Suwarso, 2002), yellowfin tuna (Thunnus albacores) from the regions of Bali, North Maluku and North Sulawesi (Permana et al, 2007), The similar study was also carried out by Wijaya et al (2010) whose fish samples were taken from Spain and Philiphine, betutu fish originating from the Brebes weir reservoir (Susanto et al, 2006), Genetic Diversity in the Population of Beronang Fish (Siganus guttatus) in the Makassar Strait and Bone Bay using the random amplified polymorphic reservoir method DNA (Rapd) (Lante et al, 2011), a study of the structure of large-eyed tuna populations in the Indian Ocean, West Sumatra, South Java and Nusa Tenggara, conducted by Suman et al (2013) and Akbar et al (2014) on genetic diversity of yellowfin tuna from two populations in the Maluku sea, Indonesia, Akbar et al (2020) on a preliminary study of the structure of the genetic population of yellow fin tuna from two populations in the Maluku sea, Indonesia, Molecular phylogenetic of grouper collecting from the traditional market (Jefri et al, 2015;Tapilatu et al, 2021;Dwifajri et al, 2022), Sardinella lemuru genetic variant in the strait waters Bali (Kartika et al, 2017) and genetic and phylogenetic variations of yellowfin tuna as a basis for sustainable fisheries management in North Maluku (Aris et al, 2017).…”

Phylogenetic and structure genetic of Rastrelliger sp in north Maluku Sea, Indonesia

“…Research on fish genetics in Indonesia has been carried out including genetic studies of populations of bigeye tuna (Thunnus obesus) in Benoa Bali [9] , kandra fish (Torsoro) originating from North Sumatra and west Java Provinces [10] , red snapper (Lutjanus malabaricus) originating from several fishing areas on the North Coast of Java and the eastern part of the Java Sea [11] , yellowfin tuna (Thunnus albacores) from the areas of Bali, North Maluku and North Sulawesi [12] , The same research has also been carried out, where fish samples were taken from Spain and the Philippines [13] , Betutu fish from the Brebes Penjalin reservoir [14] , study of the population structure of the bigeye tuna in the Indian Ocean of west Sumatra, South Java and Nusa Tenggara [15] , on the genetic diversity of yellowfin tuna from two populations in the Maluku Sea, Indonesia [5] , the preliminary study of yellowfin tuna populations from two populations in the Maluku Sea, Indonesia [16] , molecular phylogenetics of grouper genus Epinephelus from collections from traditional markets [17] , genetic variants of Sardinella lemuru in the Bali Strait sea [18] , genetic and phylogenetic variations of yellowfin tuna as the basis for sustainable fisheries management in north Maluku [19] , genetic diversity of mackerel scads, decapterus macarellus [20] in the Indian Ocean, about morphometric and genetic identification of Mackerel (Rastrelliger sp) [21] collected from Muara Baru fish market, Jakarta and about DNA barcoding and morphometric of Rastrelliger sp [22] in north Maluku Sea, Indonesia.…”