Variants of the gentamicin and tobramycin resistance plasmid pRAY are widely distributed in Acinetobacter

Hamidian, Mohammad; Nigro, Steven J.; Hall, Ruth M.

doi:10.1093/jac/dks318

Cited by 67 publications

(66 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Sequence reads were mapped to the genomes of three well-known reference strains: AYE (IC I, GenBank: NC_010410.1), ACICU (IC II, GenBank: NC_010611.1), and ATCC 17978 (sporadic ST437, GenBank: NZ_CP018664.1). An In-silico search was performed to detect the existence of 24 A. baumannii plasmid-borne replicase genes, according to the A. baumannii PCR-based replicon typing (AB-PBRT) scheme and a number of other studies on A. baumannii plasmids [19][20][21][22]. The MLST web-based search engine, hosted by the Center for Genomic Epidemiology in Denmark (http://www.genom icepi demio logy.…”

Section: Whole-genome Sequence Analysesmentioning

confidence: 99%

Molecular epidemiology and antimicrobial resistance features of Acinetobacter baumannii clinical isolates from Pakistan

Karah

Khalid

Wai

et al. 2020

Ann Clin Microbiol Antimicrob

View full text Add to dashboard Cite

Background: Acinetobacter baumannii is a Gram-negative opportunistic pathogen with a notorious reputation of being resistant to antimicrobial agents. The capability of A. baumannii to persist and disseminate between healthcare settings has raised a major concern worldwide. Methods: Our study investigated the antibiotic resistance features and molecular epidemiology of 52 clinical isolates of A. baumannii collected in Pakistan between 2013 and 2015. Antimicrobial susceptibility patterns were determined by the agar disc diffusion method. Comparative sequence analyses of the ampC and bla OXA-51-like alleles were used to assign the isolates into clusters. The whole genomes of 25 representative isolates were sequenced using the MiSeq Desktop Sequencer. Free online applications were used to determine the phylogeny of genomic sequences, retrieve the multilocus sequence types (ST), and detect acquired antimicrobial resistance genes.Results: Overall, the isolates were grouped into 7 clusters and 3 sporadic isolates. The largest cluster, Ab-Pak-cluster-1 (bla OXA-66 and ISAba1-ampC-19) included 24 isolates, belonged to ST2 and International clone (IC) II, and was distributed between two geographical far-off cities, Lahore and Peshawar. Ab-Pak-clusters-2 (bla OXA-66 , ISAba1-ampC-2), and -3 (bla OXA-66 , ISAba1-ampC-20) and the individual isolate Ab-Pak-Lah-01 (ISAba1-bla OXA-66 , ISAba1-ampC-2) were also assigned to ST2 and IC II. On the other hand, Ab-Pak-clusters-4 (bla OXA-69 , ampC-1), -5 (bla OXA-69 , ISAba1-ampC-78), and -6A (bla OXA-371 , ISAba1-ampC-3) belonged to ST1, while Ab-Pak-cluster-6B (bla OXA-371 , ISAba1-ampC-8) belonged to ST1106, with both ST1 and ST1106 being members of IC I. Five isolates belonged to Ab-Pak-cluster-7 (bla . This cluster corresponded to ST158, showed a well-delineated position on the genomic phylogenetic tree, and was equipped with several antimicrobial resistance genes including bla OXA-23 and bla . Conclusions:Our study detected the occurrence of 7 clusters of A. baumannii in Pakistan. Altogether, 6/7 of the clusters and 45/52 (86.5%) of the isolates belonged to IC I (n = 9) or II (n = 36), making Pakistan no exception to the global domination of these two clones. The onset of ST158 in Pakistan marked a geographical dispersal of this clone beyond the Middle East and brought up the need for a detailed characterization.

show abstract

Section: Whole-genome Sequence Analysesmentioning

confidence: 99%

Molecular epidemiology and antimicrobial resistance features of Acinetobacter baumannii clinical isolates from Pakistan

Karah

Khalid

Wai

et al. 2020

Ann Clin Microbiol Antimicrob

View full text Add to dashboard Cite

show abstract

“…Several genes, each conferring resistance to different combinations of aminoglycosides, have been reported in this species. 1–6 These genes occur singly or in combinations leading to a variety of resistance phenotypes. In Australian A. baumannii isolates, only five aminoglycoside resistance genes have been identified to date, namely aacC1 (conferring resistance to gentamicin), aadB (conferring resistance to gentamicin, kanamycin and tobramycin), aphA1 (conferring resistance to kanamycin and neomycin), aphA6 (conferring resistance to amikacin, kanamycin and neomycin) and aadA1 (conferring resistance to streptomycin and spectinomycin).…”

Section: Introductionmentioning

confidence: 99%

“…7,8 The aacC1 and aadA1 genes are in cassettes in a class 1 integron, and this integron is usually associated with the aphA1b gene in Tn 6020 and located in a genomic resistance island in the chromosome of most global clone 1 (GC1) strains 8 and some global clone 2 (GC2) strains. 9 However, the aadB gene cassette is often in a small plasmid, 6 and the location of the aphA6 gene is not known.…”

Section: Introductionmentioning

confidence: 99%

A GC1 Acinetobacter baumannii isolate carrying AbaR3 and the aminoglycoside resistance transposon TnaphA6 in a conjugative plasmid

Hamidian

Holt

Pickard

et al. 2013

Journal of Antimicrobial Chemotherapy

Self Cite

View full text Add to dashboard Cite

ObjectivesTo locate the acquired antibiotic resistance genes, including the amikacin resistance transposon TnaphA6, in the genome of an Australian isolate belonging to Acinetobacter baumannii global clone 1 (GC1).MethodsA multiply antibiotic-resistant GC1 isolate harbouring TnaphA6 was sequenced using Illumina HiSeq, and reads were used to generate a de novo assembly and determine multilocus sequence types (STs). PCR was used to assemble the AbaR chromosomal resistance island and a large plasmid carrying TnaphA6. Plasmid DNA sequences were compared with ones available in GenBank. Conjugation experiments were conducted.ResultsThe A. baumannii GC1 isolate G7 was shown to include the AbaR3 antibiotic resistance island. It also contains an 8.7 kb cryptic plasmid, pAb-G7-1, and a 70 100 bp plasmid, pAb-G7-2, carrying TnaphA6. pAb-G7-2 belongs to the Aci6 Acinetobacter plasmid family. It encodes transfer functions and was shown to conjugate. Plasmids related to pAb-G7-2 were detected in further amikacin-resistant GC1 isolates using PCR. From the genome sequence, isolate G7 was ST1 (Institut Pasteur scheme) and ST231 (Oxford scheme). Using Oxford scheme PCR-based methods, the isolate was ST109 and this difference was traced to a single base difference resulting from the inclusion of the original primers in the gpi segment analysed.ConclusionsThe multiply antibiotic-resistant GC1 isolate G7 carries most of its resistance genes in AbaR3 located in the chromosome. However, TnaphA6 is on a conjugative plasmid, pAb-G7-2. Primers developed to locate TnaphA6 in pAb-G7-2 will simplify the detection of plasmids related to pAb-G7-2 in A. baumannii isolates.

show abstract

“…D46 was previously shown to be ST110 (Oxford MLST) and to harbour the aphA6 amikacin resistance gene within Tn aphA6 and an aadB gene cassette (gentamicin, kanamycin and tobramycin resistance) in the small plasmid pRAY. 2 An ISAba1 upstream of the chromosomal ampC confers resistance to third-generation cephalosporins, ceftazidime and cefotaxime. 3 …”

mentioning

confidence: 99%

“…The sequence of the smallest plasmid, pD46-1 (6078 bp), contained aadB and was almost identical to pRAY* (a single base difference). 2 pD46-2 is an 8731 bp cryptic plasmid that was identical to p1ABTCDC0715 (GenBank accession number CP002523) from a GC2 isolate.…”

mentioning

confidence: 99%

Carbapenem and amikacin resistance on a large conjugative Acinetobacter baumannii plasmid

Nigro

Holt

Pickard

et al. 2014

Journal of Antimicrobial Chemotherapy

View full text Add to dashboard Cite

Variants of the gentamicin and tobramycin resistance plasmid pRAY are widely distributed in Acinetobacter

Abstract: The plasmid pRAY* and variants are widely distributed in Acinetobacter spp. and are the most common cause of resistance to gentamicin and tobramycin. Mobilization genes should assist in the dissemination of pRAY* and its variants.

Cited by 67 publications

References 11 publications

Molecular epidemiology and antimicrobial resistance features of Acinetobacter baumannii clinical isolates from Pakistan

Molecular epidemiology and antimicrobial resistance features of Acinetobacter baumannii clinical isolates from Pakistan

A GC1 Acinetobacter baumannii isolate carrying AbaR3 and the aminoglycoside resistance transposon TnaphA6 in a conjugative plasmid

Carbapenem and amikacin resistance on a large conjugative Acinetobacter baumannii plasmid

Contact Info

Product

Resources

About