Variants of Serratia marcescens Induced by Freeze-Drying

Servin-Massieu, Manuel; Cruz‐Camarillo, Ramón

doi:10.1128/aem.18.4.689-691.1969

Cited by 17 publications

(2 citation statements)

References 1 publication

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“…Moreover, this work suggests that the DNA damaged during freeze-drying can be repaired by a repair enzyme, especially an exr gene-dependent enzyme. These findings seem consistent with the results of others, who showed that freeze-drying of cells increased the mutation rate (3,12) and that drying induced temperate phages (23).…”

supporting

confidence: 93%

Deoxyribonucleic acid strand breaks during freeze-drying and their repair in Escherichia coli

Ohnishi¹,

Tanaka²,

Yoh³

et al. 1977

J Bacteriol

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Freeze-drying of Escherichia coli cells caused strand breaks of deoxyribonucleic acid (DNA) in both radiation-sensitive and -resistant strains. However, in the radiation-resistant strain E. coli B/r the damaged DNA was repaired after rehydration, whereas in the radiation-sensitive strain E. coli Bs-1 the damaged DNA was not repaired and the DNA was degraded. Repeated freeze-drying did not break the damaged DNA into smaller pieces.

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supporting

confidence: 93%

Deoxyribonucleic acid strand breaks during freeze-drying and their repair in Escherichia coli

Ohnishi¹,

Tanaka²,

Yoh³

et al. 1977

J Bacteriol

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“…In many studies the mechanisms of damage to frozen or freeze-dried cells, such as injury to cytoplasmic and outer membrane integrity (2,6,24,25), disruption of active transport and protein synthesis (8,9,30), damage to DNA (22,27,28,37), and disruption of cell morphology (5,10), have been reported.…”

Section: Discussionmentioning

confidence: 99%

Outermost-cell-surface changes in an encapsulated strain of Staphylococcus aureus after preservation by freeze-drying

Ohtomo

Yamada

Yoshida

1988

Appl Environ Microbiol

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The effects of drying time during freeze-drying on the outermost cell surface of an encapsulated strain of Staphylococcus aureus S-7 (Smith, diffuse) were investigated, with special attention paid to capsule and slime production. To quantify capsule and slime production, capsule antigen production and cellular characteristics such as growth type in serum-soft agar, cell volume index, and clumping factor reaction were examined. After freeze-drying the colonial morphology of strain S-7 was altered from a diffuse to a compact type in serum-soft agar. In accordance with these changes, the titer of the clumping factor reaction increased while the cell volume index, capsule and slime production, and capsule antigen production were markedly decreased in parallel with the period of freeze-drying. The ability of the strain to adhere to collagen, fibrinogen, and soybean lectin was also compared before and after freeze-drying. Fibrinogen levels slightly increased when 10% skim milk and 2% honey were used as cryoprotective agents and showed a remarkable increase when 0.05 M phosphate buffer was used as a control. Also, the ability of strain S-7 to adhere to soybean lectin declined, whereas no changes were observed for collagen under any conditions. Strain S-7 was phage nontypable before freeze-drying but the number of typable cells increased after freeze-drying; phage-typable cells reacted to phage 52 alone after 5 h of freeze-drying, but additional cells also proved to be phage typable to phage 42E after 10 h. Electron micrographs indicated that strain S-7, an encapsulated strain, was converted to an unencapsulated state after freeze-drying. Results of our study indicate that the freeze-drying process inhibits capsule and slime production in S. aureus, which consequently brings about changes in the outermost cell surface.

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Desiccation by Exposure to Space Vacuum or Extremely Dry Deserts: Effect on Microorganisms

Dose

2003

Encyclopedia of Environmental Microbiology

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General Limits of Growth at Low Water Activities Dormant Life Molecular Events Induced by Desiccation Survival at Extremely Low Water Activity Survival Under Extremely Dry Desert Conditions

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Variants of Serratia marcescens Induced by Freeze-Drying

Cited by 17 publications

References 1 publication

Deoxyribonucleic acid strand breaks during freeze-drying and their repair in Escherichia coli

Deoxyribonucleic acid strand breaks during freeze-drying and their repair in Escherichia coli

Outermost-cell-surface changes in an encapsulated strain of Staphylococcus aureus after preservation by freeze-drying

Desiccation by Exposure to Space Vacuum or Extremely Dry Deserts: Effect on Microorganisms

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