Abstract:Mycobacterium tuberculosis, from which the Rv0045c protein is isolated, is the most causative bacterium leading to tuberculosis. Histidine (H187), within the active site of this esterase, was the residue of interest in this project. The histidine was mutated to ten different amino acid residues (aspartate, asparagine, glycine, lysine, phenylalanine, proline, serine, tryptophan, tyrosine, and valine) and the catalytic efficiency of the enzyme variants was determined. Variant proteins were produced through site‐… Show more
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.