Variant sublines with different metastatic potentials selected in nude mice from human oral squamous cell carcinomas

Abstract

Variant sublines LMF3, LMF4 and LMF5 with high metastatic potential were established from a human oral squamous carcinoma cell line HSC-3. These sublines metastasized to the draining lymph nodes after subcutaneous inoculation into nude mice. They were obtained by sequential selection in vivo from the parent HSC-3. At each step, the cells which metastasized to lymph nodes were cultured and reinoculated into nude mice. Two other cell lines HSC-2 and HSC-4 were also established from other patients, that had neither invasive nor metastatic potential. Biologic properties were compared among high metastatic, low metastatic and non-metastatic cells. Metastatic cells grew rapidly and invaded into surrounding tissues at the inoculated site. The incidence of pulmonary colonization after intravenous injection of tumor cells was high in selected variants. Metastatic cells formed diffuse colonies in type I collagen matrix and had a higher tendency to adhere to type IV collagen network.

Tyrosine 397 phosphorylation is critical for FAK-promoted Rac1 activation and invasive properties in oral squamous cell carcinoma cells

“…FaDu is a hypopharyngeal SCC cell line, 17 whereas HSC-3 is a tongue SCC cell line with high metastatic potential. 18 Similar to the results from SCC4 and OECM-1 cells, elevated FAK expression in FaDu cells led to increased Rac1 activity, approximately fourfold higher than that in the vector control cells (Figure 3g, left panel). Treatment of PF-431396 in HSC-3 cells caused great decreases in the levels of pY397 and Rac1-GTP (Figure 3g, right panel).…”

“…Human HNSCC cell lines including SCC4, OECM-1, 16 FaDu, 17 and HSC-3 cells 18 were purchased from the BCRC (Biosource Collection and Research Center, Taiwan) and JCRB Cell Bank (Osaka, Japan), or kindly provided by Dr KW Chang (National Yang-Ming University, Taipei, Taiwan). SCC4 and OECM-1 cells were maintained in DMEM/F12 and RPMI 1640 medium, respectively.…”

Electrotaxis of oral squamous cell carcinoma cells in a multiple-electric-field chip with uniform flow field

“…43 A moderately differentiated oral squamous cell carcinoma cell line with lymph node metastasis, designated as HSC-3, originally from Japan Health Science Research Resources Bank (HSRRB, Tokyo, Japan) was a gift from Dr. Yuh-Shan Jou, Institute of Biomedical Sciences, Academia Sinica. 44,45 CL1 Cells were cultured in Dulbecco's modified Eagle Medium (DMEM, GIBCO, Invitrogen, USA), and HSC-3 cells were cultured in DMEM-F12 medium, both media supplemented with 10% fetal bovine serum (FBS, HyClone, Thermo Scientific, USA), in TCPS flasks (3290, Corning, USA) under 37 C moist atmosphere with 5% CO 2 . The cells were passaged every 3 FIG.…”

Tumour regression in a ligand inducible manner mediated by a chimeric tumour suppressor derived from p53

“…HSC-2: a gift from N Tsuchida (Momose et al, 1989); HAN, PAP: cell culture facility, IGBMC; RPMI2650: ATCC (CCL-30).…”