Variant esp gene as a marker of a distinct genetic lineage of vancomycinresistant Enterococcus faecium spreading in hospitals

Willems, Rob J. L.; Homan, Wieger; Top, Janetta; Santen-Verheuvel, Marga van; Tribe, D. E.; Manzioros, Xenia; Gaillard, Carlo A. J. M.; Vandenbroucke-Grauls, Christina M. J. E.; Mascini, Ellen M.; Kregten, E. van; Embden, J. D. A. Van; Bonten, Marc J. M.

doi:10.1016/s0140-6736(00)04205-7

Cited by 287 publications

(264 citation statements)

References 4 publications

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“…In this study, ent1 and ent2 primers (ella Biotech Gmbh, Martinsried, Germany) described by ke et al (1999) were used ( VaNkerCkhOVeN et al (2004), COque et al (1995), WILLeMS et al (2001) were used for this purpose (table 1). PCr reaction mixture and amplification conditions were carried out according to VaNkerCkhOVeN et al (2004) and BuyukyOruk et al (2014), as previously reported.…”

Section: Confirmation Of Enterococci At Genus Level By Pcr Assaymentioning

confidence: 99%

The prevalence, vancomycin resistance and virulence gene profiles of Enterococcus species recovered from different foods of animal origin

Elmali

Can

2018

Vet. arhiv

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In this study, Enterococcus faecium was the most commonly found species with a level of 10.1%, followed by Enterococcus durans (19/246, 7.7%), Enterococcus faecalis (13/246, 5.2%), and Enterococcus hirae (9/246, 3.6%). When the virulence gene profile of isolates was evaluated, gelE was the predominant (25/66, 37.8%) virulence factor in isolates followed by asa1 (22/66, 33.3%), esp (12/66, 18.1%), and cylA (4/66, 6.0%). None of the isolates harboured the hyl gene. In this study, all and/or the majority of the Enterococcus isolates tested were found to be resistant to ampicillin, rifampicin, vancomycin, and erythromycin. however, vancomycin resistance genes, such as vanB and vanC1, were not determined in any of the isolates by multiplex PCr. Only three isolates of E. durans recovered from Surk cheese were found to be carrying the vanA gene. the results demonstrate that antimicrobial-resistant and virulent strains of enterococci occur in food of animal origin in turkey.

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Section: Confirmation Of Enterococci At Genus Level By Pcr Assaymentioning

confidence: 99%

The prevalence, vancomycin resistance and virulence gene profiles of Enterococcus species recovered from different foods of animal origin

Elmali

Can

2018

Vet. arhiv

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“…This gene appears to be an enterococcus-specific virulence factor which is highly conserved in E. faecium subpopulations involved in hospital outbreaks (31), independent of the vancomycin susceptibility (23). The isolates tested in our study were negative for this element, which suggests that farm animals may not be a significant source of these genes.…”

Section: Downloaded Frommentioning

confidence: 99%

Characterization of Vancomycin-Resistant Enterococcus faecium Isolates from Broiler Poultry and Pig Farms in England and Wales

Migura-García¹,

Pleydell²,

Barnes³

et al. 2005

J Clin Microbiol

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This study aimed to investigate the occurrence and molecular epidemiology of vancomycin-resistant Enterococcus faecium (VREF) isolates on poultry and pig farms in England and Wales. A total of 217 VREF isolates were obtained from fresh feces and environmental swabs collected from conventional and organic farms. A predominant pulsed-field gel electrophoresis (PFGE) profile was found for each VREF-positive farm, together with less frequent types. All isolates presented the vanA genotype and were esp negative. Seventy-six percent of the VREF isolates were additionally resistant to nine or more antimicrobials, presenting a diverse range of resistance phenotypes. The multiresistance traits did not appear to be specific to individual farms or sample types (i.e., environmental or fecal), nor did they correlate with any specific PFGE type. Ninety-three percent of the isolates were resistant to penicillin, 89% were resistant to tetracycline, 87.5% were resistant to erythromycin, and 50% were resistant to quinupristin-dalfospristin (Synercid). The lack of clonality among these populations may suggest the horizontal transfer of resistance genes and/or a dynamic replacement of clonal lines rather than persistence.

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“…17 The esp gene is found to be enriched in infection-derived E. faecalis isolates and an esp homolog is present in E. faecium isolates. 18 Epidemiological studies have determined that esp is generally associated with and enriched among infection-derived isolates compared with commensal isolates and carried on a large pathogenicity island. 1,19,20 We, and others, 21,22 found a significant correlation between the presence of esp and the ability of enterococci to form biofilms on abiotic surfaces.…”

Section: Introductionmentioning

confidence: 99%

Surface protein Esp enhances pro-inflammatory cytokine expression through NF-κB activation during enterococcal infection

Zou

Shankar

2015

Innate Immun

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Enterococcal surface protein (Esp) is encoded on a pathogenicity island in Enterococcus faecalis and E. faecium and is involved in biofilm formation and binding to epithelial cells. In this study, using Esp-expressing E. faecalis MMH594 and its isogenic Esp-deficient strain, as well as purified Esp, we show that Esp is sufficient for activation of NF-kB and the subsequent production of pro-inflammatory cytokines IL-1b and TNF-a in macrophages in vitro. In a mouse peritonitis model, we also show that mice infected with Esp-expressing E. faecalis showed comparatively higher levels of cytokines TNF-a, IL-1b and IL-6 in peritoneal fluid, and IL-6 in serum. Moreover, neutrophil infiltration and tissue damage in the liver was higher in the mice infected with the Esp-expressing strain compared with mice infected with the Esp-deficient mutant. These results add Esp to the growing list of enterococcal virulence factors that can modulate inflammation during infection and has implications for enterococcal pathogenesis.

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Variant esp gene as a marker of a distinct genetic lineage of vancomycinresistant Enterococcus faecium spreading in hospitals

Cited by 287 publications

References 4 publications

The prevalence, vancomycin resistance and virulence gene profiles of Enterococcus species recovered from different foods of animal origin

The prevalence, vancomycin resistance and virulence gene profiles of Enterococcus species recovered from different foods of animal origin

Characterization of Vancomycin-Resistant Enterococcus faecium Isolates from Broiler Poultry and Pig Farms in England and Wales

Surface protein Esp enhances pro-inflammatory cytokine expression through NF-κB activation during enterococcal infection

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