Variação química no óleo essencial das folhas de seis indivíduos de Duguetia furfuracea (Annonaceae)

Abstract

RESUMO:Duguetia furfuracea, Annonaceae, uma invasora de pastagens, é também usada na medicina tradicional em diversos estados no Brasil. Devido à signifi cante diferença de odor entre dois grupos de indivíduos desta planta em um remanescente de cerrado em Campo Grande, Mato Grosso do Sul, seis amostras foram selecionadas de acordo com a intensidade de odor em suas folhas: In-01, In-03 e In-05: odor pronunciado (Perfi l A); In-02, In-04 e In-06: odor fraco ou ausência de odor (Perfi l B). As folhas foram coletadas e submetidas a hidrodestilação para obtenção dos óleos essenciais, os quais foram analisados por CG/EM. Uma boa compatibilidade foi encontrada entre os resultados obtidos por CG/EM e a análise olfativa adotada para as amostras: espécimes odor pronunciado apresentaram uma alta porcentagem de monoterpenos e alguns sesquiterpenos: In-01 ( -felandreno, 42,2%; mirceno, 6,8%; -felandreno, 4,6%); In-03 (terpin-4-ol, 21,6%; sabineno, 17,3%; p-cimeno, 5,6%); In-05 (sabineno, 25,1%; terpin-4-ol, 16,2%; pcimeno, 8,3%). Apenas sesquiterpenos foram encontrados nos espécimes que apresentaram odor fraco ou ausência do mesmo (com constituinte majoritário biciclogermacreno: 21,4%, 24,0%, and 29,1%, respectivamente, para os In-02, In-04 e In-06).Unitermos: Duguetia furfuracea, Annonaceae, óleo volátil.ABSTRACT: "Chemical variability of the essential oil of the leaves from six individuals of Duguetia furfuracea (Annonaceae)". Duguetia furfuracea, Annonaceae, a pasture weed, is also used in folk medicine in several Brazilian states. Because of the signifi cant difference in odor between two groups of this plant in a remnant patch of savanna in Campo Grande county, Mato Grosso do Sul, six samples were selected according to the odor intensity in the leaves (In-01, In-03, and In-05: pronounced odor; In-02, In-04, and In-06: weak odor or its absence). The leaves were collected and subjected to steam distillation for extraction of essential oils, which were analyzed by GC-MS. A good agreement was found between CG/MS results and olfactory evaluation of the samples: specimens exhibiting leaf scent had a high percentage of monoterpenes and some sesquiterpenes, as follows: In-01 ( -phellandrene, 42.2%; myrcene, 6.8%; -phellandrene, 4.6%); In-03 (terpin-4-ol, 21.6%; sabinene, 17.3%; p-cymene, 5.6%); 25.1%; 16.2%; 8.3%). Only sesquiterpenes were found in the specimens having weakly scented or scentless leaves (main constituent bicyclogermacrene: 21.4%, 24.0%, and 29.1%, respectively, for In-02, In-04 and In-06).

Chemical constituents of the volatile oil from leaves of Annona coriacea and in vitro antiprotozoal activity

“…( E)-caryophyllene is a marker in Annonaceae (Valter et al, 2008) and showed, together with δ-cadinene, a significant activity against L. donovani promastigotes (Zheljazkov et al, 2008) with an IC50 of 19 and 4 µg/mL, respectively, suggesting a probable relation to the anti-leishmanial properties of the A. coriacea oil (Table 2).…”

Chemical composition of the volatile oil of Cardiopetalum calophyllum collected in the Cerrado area

“…In a study that focused on the essential oil of Duguetia furfuracea, VALTER et al (2008) noted that the presence of oxygenated sesquiterpenes is not common in Cerrado species because of the specific conditions of the biome. However, this was not observed in the essential oil of C. calophyllum, where spathulenol was observed in high concentrations in more than one organ.…”

“…Spathulenol has been reported as the major compound in the essential oil extracted from the leaves of the other species of the Annonaceae family, such as Guatteria schomburgkiana (TRIGO et al, 2007), Annona vepretorum (COSTA et al, 2012), Rollinia leptopetala (COSTA et al, 2008) and Duguetia furfuracea (VALTER et al, 2008), among others. High concentrations of spathulenol have also been reported in the essential oil of the species of the genera Annona, Artabotrys, Cleistopholis, Goniothalamus, Piptostigma, Uvaria, and Xylopia (TRIGO et al, 2007).…”

“…The Cerrado is home to 10 genera and 47 species, none of which is endemic to the area, even though some of the genera and species are quite common, such as Annona crassiflora, Duguetia furfuracea, Xylopia aromatica, and Cardiopetalum place with respect to the number of species per family in the Cerrado. Some genera are chemically welldocumented, such as Xylopia, where significant concentrations of caryophyllene oxide, α-pinene, α-terpinene, limonene, spathulenol, germacrene-D, trans-β-guaiene, and β-caryophyllene are present in the essential oils extracted from the leaves and fruits (SANTOS, et al 2004;PONTES et al, 2007;VALTER et al, 2008). Annona genus has also been well-characterized; caryophyllene, camphene, α-pinene, and β-pinene have been identified in the essential oil extracted from the leaves (BANDEIRA et al, 2011;CAMPOS et al, 2014).…”

Four Brazilian Maytenus salicifolia Reissek (Celastraceae) groups studied by TLC and UV/Vis spectrophotometry

“…Therefore this fact shows that some kind of interactions can stimulate variations between populations of the same species with consequent alteration in the plant chemical constitution (Valter et al, 2008). The cost of fractionation processes, isolation and structural elucidation, as well as the difficulty to obtaining pure compounds that only are present in small concentrations in the plant, make these procedures virtually impossible.…”