In this paper, we present the design and implementation of two nonlinear observers: nonlinear extended state observer and sliding mode observer for estimating the pitch, yaw and roll angles and angular rates of a fixed-wing unmanned aerial vehicles system under a decoupled-reduced model in real flight experiments. A backstepping control law is designed for control in a decentralized way for altitude, yaw and roll of the airplane. This scheme allows us to test experimentally the feasibility of using the online estimated data from the observers in flight control, which is useful for increasing the robustness of the control and the safety of flight. Furthermore, a comparative analysis of the performance of both nonlinear observers is conducted.