Variable-Number Tandem Repeat Analysis of Meningococcal Isolates Belonging to the Sequence Type 162 Complex

Yazdankhah, Siamak Pour; Κεσανόπουλος, Κωνσταντίνος; Tzanakaki, Georgina; Kremastinou, Jenny; Caugant, Dominique A.

doi:10.1128/jcm.43.9.4865-4867.2005

Cited by 10 publications

(6 citation statements)

References 16 publications

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“…It has been demonstrated previously that VNTR typing is capable of distinguishing among meningococcal isolates belonging to the ST‐32, ST‐11 and ST‐162 complexes [7,8]. The present results show that VNTR analysis can distinguish outbreak‐associated isolates from sporadic cases belonging to the same ST complex.…”

Section: Phenotypic and Genotypic Data For Neisseria Meningitidis Issupporting

confidence: 71%

“…Previous studies by Yazdankhah et al. [7,8] have shown that VNTR analysis can be used for fine typing of meningococcal isolates belonging to the same clonal complex, with genetic polymorphism within the sequence type (ST) ST‐11 and ST‐162 complex of N. meningitidis revealing that they are quite heterogeneous. As ST‐162, ST‐32 and closely related STs predominate among both disease‐associated and carrier isolates in Greece [9], the present study aimed to investigate the potential of the existing conventional and molecular techniques for the identification of outbreak‐associated isolates.…”

Section: Phenotypic and Genotypic Data For Neisseria Meningitidis Ismentioning

confidence: 99%

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Conventional and molecular investigation of meningococcal isolates in relation to two outbreaks in the area of Athens, Greece

Tzanakaki

Κεσανόπουλος

Yazdankhah

et al. 2006

Clinical Microbiology and Infection

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Two local outbreaks caused by serogroup B Neisseria meningitidis occurred in the Athens area of Greece during 2003. In total, 30 N. meningitidis isolates from patients and carriers, as well as sporadic cases, were investigated by conventional techniques (serogroup, serotype and serosubtype), multilocus sequence typing (MLST), analysis of variable number tandem repeats (VNTR) and random amplified polymorphic DNA (RAPD) analysis. Compared with the two other molecular techniques, VNTR analysis was a simple, reliable and highly discriminatory method for fine typing of meningococcal isolates, showing a good correlation with the epidemiological data for the two outbreaks analysed.

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Section: Phenotypic and Genotypic Data For Neisseria Meningitidis Issupporting

confidence: 71%

Section: Phenotypic and Genotypic Data For Neisseria Meningitidis Ismentioning

confidence: 99%

Conventional and molecular investigation of meningococcal isolates in relation to two outbreaks in the area of Athens, Greece

Tzanakaki

Κεσανόπουλος

Yazdankhah

et al. 2006

Clinical Microbiology and Infection

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“…The relevance of this study is related to the high incidence, in Greece, of cc162, which is rare in Europe. cc162 has been described to be present both in disease-associated and in carrier isolates in Greece, with a high degree of heterogeneity among the isolates [ 35 , 36 ].…”

Section: Resultsmentioning

confidence: 99%

Diversity of greek meningococcal serogroup B isolates and estimated coverage of the 4CMenB meningococcal vaccine

et al. 2014

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BackgroundSerogroup B meningococcal (MenB) isolates currently account for approximately 90% of invasive meningococcal disease (IMD) in Greece with ST-162 clonal complex predominating. The potential of a multicomponent meningococcal B vaccine (4CMenB) recently licensed in Europe was investigated in order to find whether the aforementioned vaccine will cover the MenB strains circulating in Greece. A panel of 148 serogroup B invasive meningococcal strains was characterized by multilocus sequence typing (MLST) and PorA subtyping. Vaccine components were typed by sequencing for factor H-binding protein (fHbp), Neisserial Heparin Binding Antigen (NHBA) and Neisseria adhesin A (NadA). Their expression was explored by Meningococcal Antigen Typing System (MATS).ResultsGlobal strain coverage predicted by MATS was 89.2% (95% CI 63.5%-98.6%) with 44.6%, 38.5% and 6.1% of strains covered by one, two and three vaccine antigens respectively. NHBA was the antigen responsible for the highest coverage (78.4%), followed by fHbp (52.7%), PorA (8.1%) and NadA (0.7%). The coverage of the major genotypes did not differ significantly. The most prevalent MLST genotype was the ST-162 clonal complex , accounting for 44.6% of the strains in the panel and with a predicted coverage of 86.4%, mainly due to NHBA and fHbp.Conclusions4CMenB has the potential to protect against a significant proportion of Greek invasive MenB strains.

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“…Recently, Yazdankhah et al presented two studies on the use of MLVA for molecular typing of N. meningitidis (37,39). In their reports, they show that their MLVA can be used for fine typing of meningococcal strains and that outbreaks can be delineated by VNTR analysis.…”

Section: Discussionmentioning

confidence: 99%

“…To type a single strain, seven PCRs and 14 sequence reactions are required. Recently, multiple-locus variable-number tandem repeat analysis (MLVA) has been introduced as a typing method for a large number of bacterial pathogens (6-8, 10, 16, 17, 19, 23, 26, 28, 29, 32, 34, 35) and meningococci (37,39). In MLVA, the variability in the numbers of short tandem repeated sequences is utilized to create DNA fingerprints for epidemiological studies.…”

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confidence: 99%

Multiple-Locus Variable-Number Tandem Repeat Analysis of Neisseria meningitidis Yields Groupings Similar to Those Obtained by Multilocus Sequence Typing

et al. 2006

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We identified many variable-number tandem repeat (VNTR) loci in the genomes of Neisseria meningitidis serogroups A, B, and C and utilized a number of these loci to develop a multiple-locus variable-number tandem repeat analysis (MLVA). Eighty-five N. meningitidis serogroup B and C isolates obtained from Dutch patients with invasive meningococcal disease and seven reference strains were analyzed using MLVA and multilocus sequence typing (MLST). MLVA, based on eight VNTR loci with limited variability in the number of repeats, yielded clustering of the strains similar to that obtained by MLST, with congruence between both methods amounting to 69%. The ability to recognize clonal complexes makes MLVA a valuable high-throughput method to serve as a tool complementary to MLST. Four highly variable VNTR loci were used in a second assay to analyze N. meningitidis serogroup C strains collected during an outbreak of meningococcal disease in The Netherlands. Typing based on the latter VNTR loci enabled differentiation of isolates with identical MLST sequence types and grouped epidemiologically related strains.Neisseria meningitidis remains a major cause of meningitis and septicemia worldwide (4, 24). On the basis of the structure of its capsule polysaccharide, 13 serogroups are recognized. Polysaccharide vaccines against serogroups A, C, Y, and W135 are available. Due to poor immunogenicity and cross-reactivity with neural tissue, a vaccine based on the serogroup B polysaccharide is not available. A licensed vaccine against serogroup B meningococci based on other components of the pathogen will not become available for some time. While disease due to serogroup A, W135, and C meningococci is prevalent in Africa and Asia, in Europe and the Americas serogroup B meningococci are causing most of the cases of meningococcal disease. Study of the epidemiology of N. meningitidis increases knowledge about the spread of the bacterium and has identified particular clones with apparent increased virulence (15,20). Many different typing techniques have been employed to characterize meningococci. This is particularly true for the molecular techniques, which range from multilocus enzyme electrophoresis to PorA variable region typing and multilocus sequence typing (MLST) (1,3,5,20,27,33). MLST can now be considered the gold standard for genotyping N. meningitidis, and a large database is accessible via the Internet (http://pubmlst.org/neisseria/). MLST of N. meningitidis is a method using sequence data obtained from seven housekeeping genes. The alleles from these housekeeping genes are assigned allele numbers, and the combination of these allele numbers makes up a sequence type. MLST is a portable technique yielding unambiguous results and has been shown to be very suited for global epidemiology of meningococci (9,20,21,31). However, despite these obvious advantages, MLST is a costly and labor-intensive typing technique. To type a single strain, seven PCRs and 14 sequence reactions are required. Recently, multiple-locus variable-number ta...

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Variable-Number Tandem Repeat Analysis of Meningococcal Isolates Belonging to the Sequence Type 162 Complex

Cited by 10 publications

References 16 publications

Conventional and molecular investigation of meningococcal isolates in relation to two outbreaks in the area of Athens, Greece

Conventional and molecular investigation of meningococcal isolates in relation to two outbreaks in the area of Athens, Greece

Diversity of greek meningococcal serogroup B isolates and estimated coverage of the 4CMenB meningococcal vaccine

Multiple-Locus Variable-Number Tandem Repeat Analysis of Neisseria meningitidis Yields Groupings Similar to Those Obtained by Multilocus Sequence Typing

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