Variable Adhesion of Listeria monocytogenes Isolates from Food-Processing Facilities and Clinical Cases to Inert Surfaces

Tresse, Odile; Shannon, Kelly; Pinon, Anthony; Malle, Pierre; Vialette, Michèle; Midelet, Graziella

doi:10.4315/0362-028x-70.7.1569

Cited by 61 publications

(46 citation statements)

References 36 publications

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“…The inconsistent results obtained could be due to differences in strains, media, and environmental conditions during biofilm formation (45). It is interesting to investigate the ability of serotype 1/2a and 4b strains to form biofilms because serotype 1/2a strains are frequently isolated from food-processing environments but serotype 4b strains are commonly implicated in food-borne outbreaks of listeriosis (1,3,14,15,17,22,29,31,41,47,49).…”

Section: Discussionmentioning

confidence: 99%

Competition of Listeria monocytogenes Serotype 1/2a and 4b Strains in Mixed-Culture Biofilms

Pan

Breidt

Kathariou

2009

Appl Environ Microbiol

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The majority of Listeria monocytogenes isolates recovered from foods and the environment are strains of serogroup 1/2, especially serotypes 1/2a and 1/2b. However, serotype 4b strains cause the majority of human listeriosis outbreaks. Our investigation of L. monocytogenes biofilms used a simulated food-processing system that consisted of repeated cycles of growth, sanitation treatment, and starvation to determine the competitive fitness of strains of serotypes 1/2a and 4b in pure and mixed-culture biofilms. Selective enumeration of strains of a certain serotype in mixed-culture biofilms on stainless steel coupons was accomplished by using serotypespecific quantitative PCR and propidium monoazide treatment to prevent amplification of extracellular DNA or DNA from dead cells. The results showed that the serotype 1/2a strains tested were generally more efficient at forming biofilms and predominated in the mixed-culture biofilms. The growth and survival of strains of one serotype were not inhibited by strains of the other serotype in mixed-culture biofilms. However, we found that a cocktail of serotype 4b strains survived and grew significantly better in mixed-culture biofilms containing a specific strain of serotype 1/2a (strain SK1387), with final cell densities averaging 0.5 log 10 CFU/cm 2 higher than without the serotype 1/2a strain. The methodology used in this study contributed to our understanding of how environmental stresses and microbial competition influence the survival and growth of L. monocytogenes in pure and mixed-culture biofilms.

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Section: Discussionmentioning

confidence: 99%

Competition of Listeria monocytogenes Serotype 1/2a and 4b Strains in Mixed-Culture Biofilms

Pan

Breidt

Kathariou

2009

Appl Environ Microbiol

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“…L. monocytogenes Scott A (serotype 4b, epidemic strain, human isolate), kindly provided by Eddy Smid (Agrotechnological Research Institute ATO-DLO, Wageningen, The Netherlands), was used throughout the study. Although L. monocytogenes Scott A does not attach to surfaces as strongly as other strains, its attachment ability has been ranked in the middle of those of a list of multiple clinical and food isolates (12,49). This strain was selected due to its clinical origin and the strong epidemiologic association of serotype 4b with human listeriosis (25,29).…”

Section: Methodsmentioning

confidence: 99%

Listeria monocytogenes Attachment to and Detachment from Stainless Steel Surfaces in a Simulated Dairy Processing Environment

Poimenidou¹,

Belessi²,

Giaouris

et al. 2009

Appl Environ Microbiol

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The presence of pathogens in dairy products is often associated with contamination via bacteria attached to food-processing equipment, especially from areas where cleaning/sanitation is difficult. In this study, the attachment of Listeria monocytogenes on stainless steel (SS), followed by detachment and growth in foods, was evaluated under conditions simulating a dairy processing environment. Initially, SS coupons were immersed in milk, vanilla custard, and yogurt inoculated with the pathogen (10 7 CFU/ml or CFU/g) and incubated at two temperatures (5 and 20°C) for 7 days. By the end of incubation, cells were mechanically detached from coupons and used to inoculate freshly pasteurized milk which was subsequently stored at 5°C for 20 days. The suspended cells in all three products in which SS coupons were immersed were also used to inoculate freshly pasteurized milk (5°C for 20 days). When SS coupons were immersed in milk, shorter lag phases were obtained for detached than for planktonically grown cells, regardless of the preincubation temperature (5 or 20°C). The opposite was observed when custard incubated at 20°C was used to prepare the two types of inocula. However, in this case, a significant increase in growth rate was also evident when the inoculum was derived from detached cells. In another parallel study, while L. monocytogenes was not detectable on SS coupons after 7 days of incubation (at 5°C) in inoculated yogurt, marked detachment and growth were observed when these coupons were subsequently transferred and incubated at 5°C in fresh milk or/and custard. Overall, the results obtained extend our knowledge on the risk related to contamination of dairy products with detached L. monocytogenes cells.

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“…In particular, the inability of the microtiter plate assay to reveal the HB phenotype raises questions about the utility of the microtiter plate assay to provide clear predictions about how L. monocytogenes will behave in natural and industrial settings. On the basis of this methodology, a number of reports have drawn conclusions about the potential for different clinical and environmental isolates to form biofilms (1,5,15). It may be that the same strains would be scored differently if they were assessed in a flow cell system.…”

mentioning

confidence: 99%

Loss of Flagellum-Based Motility by Listeria monocytogenes Results in Formation of Hyperbiofilms

Todhanakasem

Young

2008

J Bacteriol

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Biofilm formation by the gram-positive, motile, food-borne pathogen Listeria monocytogenes was demonstrated to occur by an ordered series of stages. Biofilm development involves flagellum-based motility, which when blocked decreases initial bacterial surface attachment but subsequently leads to the formation of hyperbiofilms, surface-attached communities reaching high density.Listeria monocytogenes has emerged as a major foodborne pathogen posing a major public health concern because listeriosis has a fatality rate of 25% (13). Therefore, efforts to limit human exposure to L. monocytogenes that focus on defining mechanisms affecting its entry into the food supply have substantial public health value. The survival of L. monocytogenes in the food processing environment is prolonged because of its ability to establish biofilms on the surfaces of equipment. This is postulated to be a major reservoir contributing to food contamination and disease transmission (6,19

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Variable Adhesion of Listeria monocytogenes Isolates from Food-Processing Facilities and Clinical Cases to Inert Surfaces

Cited by 61 publications

References 36 publications

Competition of Listeria monocytogenes Serotype 1/2a and 4b Strains in Mixed-Culture Biofilms

Competition of Listeria monocytogenes Serotype 1/2a and 4b Strains in Mixed-Culture Biofilms

Listeria monocytogenes Attachment to and Detachment from Stainless Steel Surfaces in a Simulated Dairy Processing Environment

Loss of Flagellum-Based Motility by Listeria monocytogenes Results in Formation of Hyperbiofilms

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