Variability of the NS(S) protein among Rift Valley fever virus isolates.

Sall, Amadou Alpha; Zanotto, Paolo Marinho de Andrade; Zeller, H.; Digoutte, J. P.; Thiongane, Yaya; Bouloy, Michèle

doi:10.1099/0022-1317-78-11-2853

Cited by 51 publications

(47 citation statements)

References 30 publications

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“…The demonstration here of incongruities among the RVF virus S, M, and L segment tree topologies, together with earlier such findings based on partial genome sequences, indicates that RVF virus RNA segment reassortment does occur in nature (53,54). The dominance of a single viral genotype during epizootic/epidemic periods, the short duration of infected-host viremia, and the strongly crossprotective and sterilizing immunity elicited by RVF virus infection in livestock and humans all point to a relatively small (17,47).…”

Section: ϫ4mentioning

confidence: 74%

Complete Genome Analysis of 33 Ecologically and Biologically Diverse Rift Valley Fever Virus Strains Reveals Widespread Virus Movement and Low Genetic Diversity due to Recent Common Ancestry

Bird

Khristova

Rollin

et al. 2007

J Virol

215

270

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؊4 substitutions/site/year, although some evidence of RNA segment reassortment was found. Bayesian analysis of current RVF virus genetic diversity places the most recent common ancestor of these viruses in the late 1800s, the colonial period in Africa, a time of dramatic changes in agricultural practices and introduction of nonindigenous livestock breeds. In addition to insights into the evolution and ecology of RVF virus, these genomic data also provide a foundation for the design of molecular detection assays and prototype vaccines useful in combating this important disease.

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Section: ϫ4mentioning

confidence: 74%

Complete Genome Analysis of 33 Ecologically and Biologically Diverse Rift Valley Fever Virus Strains Reveals Widespread Virus Movement and Low Genetic Diversity due to Recent Common Ancestry

Bird

Khristova

Rollin

et al. 2007

J Virol

215

270

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“…As far as RVF is concerned, Senegal can be divided in two areas (Sall et al 1997b) from which the virus have emerged as demonstrated by isolations in 1993 (Zeller et al 1997): (i) the Sahelian zone, where southern Mauritanian and northern Senegalese strains are circulating and, (ii) the Sudano-Guinean zone where southern Senegalese strains are in contact with those from bordering countries.…”

Section: Modes Of Circulation Of Rvfvmentioning

confidence: 99%

“…To further analyze the genetic diversity of RVFV (Sall et al 1997b), we selected a panel of 18 strains (Table I) isolated over some 50 years from various hosts and geographical origins and we sequenced directly their NSs coding region on the S segment after a step of reverse transcription-polymerase chain reaction amplification (RT/PCR). A 50% majority rule consensus tree derived from the sequences analyzed are presented in Fig.…”

Section: Molecular Epidemiology Of Rvfvmentioning

confidence: 99%

Molecular Epidemiology and Emergence of Rift Valley Fever

Sall

Zanotto

Vialat

et al. 1998

Mem. Inst. Oswaldo Cruz

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“…To test the specificity of the amplification, we carried out the reaction with RNAs from RVFV strains clustered in the three groups referred to as West Africa, East-Central Africa, and Egypt (31,32,34). These strains were ZH501, ZH548, ArD38661, 74HB59, and clone 13.…”

Section: Real-time Rtd-pcr Of Rvfvmentioning

confidence: 99%

Quantitative Real-Time PCR Detection of Rift Valley Fever Virus and Its Application to Evaluation of Antiviral Compounds

et al. 2001

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The Rift Valley fever virus (RVFV), a member of the genus Phlebovirus (family Bunyaviridae) is an enveloped negative-strand RNA virus with a tripartite genome. Until 2000, RVFV circulation was limited to the African continent, but the recent deadly outbreak in the Arabian Peninsula dramatically illustrated the need for rapid diagnostic methods, effective treatments, and prophylaxis. A method for quantifying the small RNA segment by a real-time detection reverse transcription (RT)-PCR using TaqMan technology and targeting the nonstructural protein-coding region was developed, and primers and a probe were designed. After optimization of the amplification reaction and establishment of a calibration curve with synthetic RNA transcribed in vitro from a plasmid containing the gene of interest, real-time RT-PCR was assessed with samples consisting of RVFV from infected Vero cells. The method was found to be specific for RVFV, and it was successfully applied to the detection of the RVFV genome in animal sera infected with RVFV as well as to the assessment of the efficiency of various drugs (ribavirin, alpha interferon, 6-azauridine, and glycyrrhizin) for antiviral activity. Altogether, the results indicated a strong correlation between the infectious virus titer and the amount of viral genome assayed by real time RT-PCR. This novel method could be of great interest for the rapid diagnosis and screening of new antiviral compounds, as it is sensitive and time saving and does not require manipulation of infectious material.The Rift Valley fever (RVF) virus (RVFV), a member of the genus Phlebovirus, belongs to the Bunyaviridae family and possesses a negative-stranded, tripartite RNA genome composed of a large, a medium, and a small (S) segment (for reviews, see references 9 and 37). Like other phleboviruses, the S segment utilizes an ambisense strategy to code for two proteins, the nucleocapsid protein and the nonstructural protein (NS s ), which are synthesized from subgenomic viral complementary and viral sense mRNA, respectively.RVF is a mosquito-borne zoonosis predominantly provoking the death of young animals and abortion (e.g., sheep and goats) (for reviews, see references 24, 39 and 41). The disease was first identified in sheep by Daubney et al. in Kenya in 1931, and it is endemic almost everywhere in subtropical Africa (6). Transmission to humans occurs primarily by contact with infected animal body fluids and by mosquito bites. Infection is usually asymptomatic or associated with a brief self-limited febrile illness. However, complications such as retinitis, encephalitis, or hemorrhagic fever occur in some patients with mortality rates of up to 10 to 12% (21, 28).The potential of RVF as a disease emerging in new areas was first documented in Egypt in 1977 (16), and since then, epidemics have occurred in Mauritania (1987Mauritania ( to 1988Mauritania ( and 1998, Madagascar (1990to 1991), Egypt (1993, and eastern Africa (in Kenya, Somalia, and Tanzania) (references 33 and 34 and references therein). Recently, the...

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Variability of the NS(S) protein among Rift Valley fever virus isolates.

Cited by 51 publications

References 30 publications

Complete Genome Analysis of 33 Ecologically and Biologically Diverse Rift Valley Fever Virus Strains Reveals Widespread Virus Movement and Low Genetic Diversity due to Recent Common Ancestry

Complete Genome Analysis of 33 Ecologically and Biologically Diverse Rift Valley Fever Virus Strains Reveals Widespread Virus Movement and Low Genetic Diversity due to Recent Common Ancestry

Molecular Epidemiology and Emergence of Rift Valley Fever

Quantitative Real-Time PCR Detection of Rift Valley Fever Virus and Its Application to Evaluation of Antiviral Compounds

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