Variability of the mitochondrial CO1 gene in native and invasive populations of Harmonia axyridis Pall. comparative analysis

Abstract

Our study is focused on original and publicly accessible data on the intraspecific variability of the barcoding DNA fragment in ladybirds Harmonia axyridis Pall analysis. The complete dataset consists of 39 haplotypes, 16 of which we identified for the first time. The intra-population and geographical variability of the barcoding fragment was studied for seven populations of the western and eastern groups of the native range and in six invasive populations, in which 25 of the 39 haplotypes are found. Population structure inferred on base of molecular variability and haplotype frequencies showed a high level of differences between the eastern and western groups of native populations and confirm the hypothesis of the origin of all invasive populations from native populations of the eastern group. A comparative analysis of molecular variation indices testifies to various evolutionary scenarios of the formation of the western and eastern groups of native populations and confirms the hypothesis of the microevolutionary history of the species, previously suggested in morphological character based studies of the geographical variability of H. axyridis. A significant decrease in the molecular diversity of invasive populations confirms the hypothesis of a random nature of the primary invasion of this species in North America. OPEN ACCESS Citation: Blekhman A, Goryacheva I, Schepetov D, Zakharov I (2020) Variability of the mitochondrial CO1 gene in native and invasive populations of Harmonia axyridis Pall. сomparative analysis. PLoS ONE 15(4): e0231009. https://doi.org/10.

CO1 barcodes resolve an asymmetric biphyletic clade for Diabrotica undecimpunctata subspecies and provide nucleotide variants for differentiation from related lineages using real-time PCR

“…For example, within the species D. adelpha and D. limitata, haplotypes were separated by 53 and 28 SNVs, respectively (Figure 2). The publicly available CO1 sequences for D. biannularis, D. balteata, D. speciosa, and D. tibialis were, by contrast, similar to what is generally observed for intraspecific variability in beetles and other insects (49,63), although increased sampling is needed to more fully describe the nucleotide variability in these Diabrotica lineages. Given the discrepancies observed between taxonomic designation and CO1 haplotypes, both here and in other phylogenetic appraisals of Diabrotica and allied species (22), a concerted effort should be made to better understand how the sorting and evolution of mitochondrial and other genetic and genomic data corresponds to morphological, behavioral, and adaptive divergence.…”

The evolutionary dynamics of biological invasions: A multi‐approach perspective

“…Haplotype diversity estimated from single DNA locus (mitochondrial DNA) reveals a reduced number of haplotypes among invasive populations as compared to the native ones (Blekhman et al, 2020) confirming their most likely same NAM origin (Blekhman et al, 2020;Lombaert et al, 2010). Diversity estimates from multilocus microsatellites support the role of introduction modalities with lower allelic richness in single introductions from NAM (SAM and SAF) and higher expected heterozygosity in EU than in both parents (BIO and NAM) .…”

“…Figures adapted from cited literature. References [1] Lombaert et al (2010); [2] Blekhman et al (2020); [3] Lombaert, Guillemaud, et al (2014)); [4] Facon, Hufbauer, et al (2011)); [5] Facon, Crespin, et al (2011)); [6] Turgeon et al (2011); [7] Tayeh et al (2013); [8] Laugier, (2013).…”

Interactions Among Native and Non-Native Predatory Coccinellidae Influence Biological Control and Biodiversity

“…Analysis of mitochondrial CO1 of H . axyridis showed significant differences between the eastern and western populations in the native Russian range, suggesting that all populations in the invaded North American and European regions originated from populations from eastern Asia ( Blekhman et al 2020 ). Examination of single-copy nuclear genes of H. axyridis populations across mainland China and North America, South America, and Europe indicated that the genetic diversity in China was higher than that in North America and Europe, but lower than that in South America ( Li 2020 ).…”