Variability of seed traits and properties of soluble mucilages in lines of the flax genetic collection of Vavilov Institute

Павлов, А. В.; Paynel, Florence; Rihouey, Christophe; Porokhovinova, E. A.; Brutch, N. B.; Morvan, Claudine

doi:10.1016/j.plaphy.2014.04.020

Cited by 10 publications

(13 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…4c). Monosaccharide analysis also confirmed previous findings that mucilage fractions from A. thaliana and L. usitatissimum are rich in rhamnose and galacturonic acid [4,[47][48][49][50], components of pectin, a highly water-soluble polysaccharide, the presence of which may contribute to overall ease of extraction in these species. It is the presence of minor mucilage components in the HWE and IAE fractions that are known to affect the mucilage properties including fractionality.…”

Section: The Extraction Pipeline Effectively Provides Materials For Idsupporting

confidence: 86%

A small-scale fractionation pipeline for rapid analysis of seed mucilage characteristics

et al. 2020

View full text Add to dashboard Cite

Background: Myxospermy is a process by which the external surfaces of seeds of many plant species produce mucilage-a polysaccharide-rich gel with numerous fundamental research and industrial applications. Due to its functional properties the mucilage can be difficult to remove from the seed and established methods for mucilage extraction are often incomplete, time-consuming and unnecessarily wasteful of precious seed stocks. Results: Here we tested the efficacy of several established protocols for seed mucilage extraction and then downsized and adapted the most effective elements into a rapid, small-scale extraction and analysis pipeline. Within 4 h, three chemically-and functionally-distinct mucilage fractions were obtained from myxospermous seeds. These fractions were used to study natural variation and demonstrate structure-function links, to screen for known mucilage quality markers in a field trial, and to identify research and industry-relevant lines from a large mutant population. Conclusion: The use of this pipeline allows rapid analysis of mucilage characteristics from diverse myxospermous germplasm which can contribute to fundamental research into mucilage production and properties, quality testing for industrial manufacturing, and progressing breeding efforts in myxospermous crops.

show abstract

Section: The Extraction Pipeline Effectively Provides Materials For Idsupporting

confidence: 86%

A small-scale fractionation pipeline for rapid analysis of seed mucilage characteristics

et al. 2020

View full text Add to dashboard Cite

show abstract

“…This finding might be ascribed to extraction conditions employed (Wang et al, 2010). Moreover, environmental factors, such as climate, and crop maturity might also affect FG yield (Saag et al, 1975;Diederichsen et al, 2006;Pavlov et al, 2014). Extraction yield is an important parameter in determining the economic viability of FG recovery while it is also desirable to determine functional properties.…”

Section: Fg Yieldmentioning

confidence: 99%

“…FG properties are dependent on extraction conditions, drying processes, shear and extrusion, as well as flaxseed cultivar used as a source Wang et al, 2009aWang et al, , 2010Wang et al, , 2011a. The influence of flaxseed cultivar on FG content and functional properties has been investigated (Wannerberger et al, 1991;Bhatty, 1993;Fedeniuk & Biliaderis, 1994;Oomah et al, 1995;Diederichsen et al, 2006;Pavlov et al, 2014). FG quality was characterised by a mucilage indicator value (MIV), but this was not correlated with other flaxseed characters, such as seed mass or colour, or even an accession's geographic origin (Diederichsen et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Variation of composition and functional properties of gum from six Canadian flaxseed (Linum usitatissimum L.) cultivars

Shim

Shen

Yong

et al. 2016

Int J of Food Sci Tech

View full text Add to dashboard Cite

Summary Flaxseed gum (FG) was prepared from six Canadian flaxseed (Linum usitatissimum L.) cultivars. Yield, neutral sugar content, acidic sugar content and protein content varied significantly among cultivar FG extracts. Zeta potential (−16.4 to −27.4 mV) and rheological properties of solution were cultivar dependent. Solutions prepared with CDC Glas FG had the highest viscosity (2.984 Pa•s), while CDC Sorrel produced the lowest (0.048 Pa•s). FG solutions exhibited pseudoplastic shear‐thinning behaviour. Viscosity of FG solution increased with concentration, while viscosity and pseudoplasticity decreased with increased temperature. NaCl addition decreased solution viscosity while adding sucrose increased viscosity. FG solutions prepared from Flanders and CDC Sorrel had the highest and lowest emulsion activity index, respectively, while solutions of CDC Arras and CDC Bethune FG had the lowest and highest emulsion stability, respectively. Findings presented here provide information that could enable greater utilisation in food, cosmetic and pharmaceutical industries as thickeners and/or emulsifiers.

show abstract

“…The same process is applied in parallel for the seeds. So far, despite the fact that no correlation was found between the area of the seeds and the quantity of soluble mucilage extracted [84], we assumed that the soluble mucilage area may potentially be linked to the seed size and/or the seed shape. We decided to normalize the area of each soluble mucilage by the area of their respective seed (AreaS).…”

Section: Resultsmentioning

confidence: 99%

“…5a). To check it, flaxseeds were immersed in water for 24 h in order to extract the soluble part of the mucilage [7, 84] and seeds were then placed on a biochemical assay. Since a thin halo of mucilage is still detected arround the seed (Fig.…”

Section: Resultsmentioning

confidence: 99%

MuSeeQ, a novel supervised image analysis tool for the simultaneous phenotyping of the soluble mucilage and seed morphometric parameters

et al. 2018

View full text Add to dashboard Cite

BackgroundThe mucilage is a model to study the polysaccharide biosynthesis since it is produced in large amounts and composed of complex polymers. In addition, it is of great economic interest for its technical and nutritional value. A fast method for phenotyping the released mucilage and the seed morphometric parameters will be useful for fundamental, food, pharmaceutical and breeding researches. Current strategies to phenotype soluble mucilage are restricted to visual evaluations or are highly time-consuming.ResultsHere, we developed a high-throughput phenotyping method for the simultaneous measurement of the soluble mucilage content released on a gel and the seed morphometric parameters. Within this context, we combined a biochemical assay and an open-source computer-aided image analysis tool, MuSeeQ. The biochemical assay consists in sowing seeds on an agarose medium containing the dye toluidine blue O, which specifically stains the mucilage once it is released on the gel. The second part of MuSeeQ is a macro developed in ImageJ allowing to quickly extract and analyse 11 morphometric data of seeds and their respective released mucilages. As an example, MuSeeQ was applied on a flax recombinant inbred lines population (previously screened for fatty acids content.) and revealed significant correlations between the soluble mucilage shape and the concentration of some fatty acids, e.g. C16:0 and C18:2. Other fatty acids were also found to correlate with the seed shape parameters, e.g. C18:0 and C18:2. MuSeeQ was then showed to be used for the analysis of other myxospermous species, including Arabidopsis thaliana and Camelina sativa.ConclusionsMuSeeQ is a low-cost and user-friendly method which may be used by breeders and researchers for phenotyping simultaneously seeds of specific cultivars, natural variants or mutants and their respective soluble mucilage area released on a gel. The script of MuSeeQ and video tutorials are freely available at http://MuSeeQ.free.fr.Electronic supplementary materialThe online version of this article (10.1186/s13007-018-0377-5) contains supplementary material, which is available to authorized users.

show abstract

Variability of seed traits and properties of soluble mucilages in lines of the flax genetic collection of Vavilov Institute

Cited by 10 publications

References 39 publications

A small-scale fractionation pipeline for rapid analysis of seed mucilage characteristics

A small-scale fractionation pipeline for rapid analysis of seed mucilage characteristics

Variation of composition and functional properties of gum from six Canadian flaxseed (Linum usitatissimum L.) cultivars

MuSeeQ, a novel supervised image analysis tool for the simultaneous phenotyping of the soluble mucilage and seed morphometric parameters

Contact Info

Product

Resources

About