Variability of light‐evoked response pattern and morphological characterization of amacrine cells in goldfish retina

Djamgoz, Mustafa B.A.; Spadavecchia, L.; Usai, Cesare; Vallerga, S.

doi:10.1002/cne.903010204

Cited by 27 publications

(28 citation statements)

References 42 publications

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“…We find similar fast events in cat. 4) In goldfish and turtle retinas, axon-like processes have been observed to extend from the conventional dendritic arbors of ON-OFF amacrine cells, similar to our finding in cat (Ammermuller and Weiler, 1989;Djamgoz et al, 1990;Ammermuller and Kolb, 1995). It is possible that such processes extend from ON-OFF amacrine cells in most other species, too, but that they have been missed because they are so thin.…”

Section: Comparison To On-off Amacrine Cells In Other Speciessupporting

confidence: 82%

ON-OFF amacrine cells in cat retina

et al. 1996

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We studied the morphology, photic responses, and synaptic connections of ON-OFF amacrine cells in the cat retina by penetrating them with intracellular electrodes, staining them with horseradish peroxidase, and examining them with the electron microscope. In a sample of seven cells, we found two different morphological types: the A19, which ramifies narrowly in stratum 2 (sublamina a) of the inner plexiform layer, and the A22, which ramifies mostly in stratum 4 (sublamina b) but extends some dendrites to sublamina a. Both of these cell types have axon-like processes that extend > 800 microns from the conventional dendritic arbor. ON-OFF amacrine cells in our sample had receptive fields (1.7 +/- 0.3 mm diameter) that were broader than their dendritic arbors (425 +/- 35 microns diameter) and that extended over the region of axon-like processes. In addition, we found many features in common with ON-OFF amacrine cells in poikilotherm vertebrates: a broad receptive field without surround antagonism, two sizes of spike-like events, narrow dynamic range (1 log unit intensity), and excitatory postsynaptic potentials at light on and light off. Two A19 amacrine cells were examined in the electron microscope: most synaptic inputs (93 and 76%, respectively) to either cell were from amacrine cells, with minor inputs from cone bipolar cells. Synaptic outputs were to bipolar, amacrine, and ganglion cells, including the OFF-alpha cell.

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Section: Comparison To On-off Amacrine Cells In Other Speciessupporting

confidence: 82%

ON-OFF amacrine cells in cat retina

et al. 1996

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“…They were said to exhibit color-opponent centers but nonopponent surrounds (Yazulla, 197613). With this knowledge of a high degree of color processing going on in the turtle OPL, we might then also expect to see coloropponent responses in some of the amacrine cells of the IPL, as has been reported for fish (Kaneko, 1973;Mitarai et al, 1978;Djamgoz and Ruddock, 1983;Kaneko and Tachibana, 1983;Watanabe and Murakami, 1985;Djamgoz et al, 1990). However, no such data are available for turtle retina.…”

mentioning

confidence: 81%

“…At the OPL, chromaticity was found in horizontal and bipolar cells (Miller et al, 1973;Fuortes and Simon, 1974;Yazulla, 1976a,b;Ohtsuka and Kouyama, 1986) and in the IPL in ganglion cells (Bowling, 1980;Marchiafava and Wagner, 1981;Marchiafava, 1983;Granda and Fulbrook, 1989). Until now, color-coded amacrine cells had not been reported in the turtle retina, although they are known to exist in other species such as fish (Kaneko, 1973;Mitarai et al, 1978;Djamgoz and Ruddock, 1983;Watanabe and Murakami, 1985;Djamgoz et al, 1990).…”

Section: Color Coding In Turtle Retinal Neuronsmentioning

confidence: 99%

The organization of the turtle inner retina. II. Analysis of color‐coded and directionally selective cells

Ammermüller

Kolb

1995

J of Comparative Neurology

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Color coding and directional selectivity (DS) of retinal neurons were studied in the Pseudemys turtle by using similar intracellular recording and staining techniques as in the preceding paper (J. Ammermüller and H. Kolb, 1995, J. Comp. Neuronal. 358:1-34). Color-coded responses were elicited by red (621 or 694 nm), green (525 or 514 nm), and blue (455 nm) light flashes. In addition to red/green and yellow/blue types of chromaticity horizontal cells, in our sample of 305 identified cells we found that 17% of bipolar cells, 6.5% of amacrine cells, and 18% of ganglion cells exhibit color-coded responses. DS responses were found in 37% of the tested ganglion cells and 41% of the tested amacrine cells. Two morphologically identified bipolar cell types, B10 and B11, were red-ON/blue-OFF and red-OFF/green, blue-ON, respectively. Of five identified amacrine cell types, three were red-OFF/blue-ON center (A1, A3, A23b), one was red-OFF/green-ON center (A32), and one (A33) was double color-opponent of red-ON/blue-OFF center:red-OFF/blue-ON surround. Five ganglion cell types had variously color-coded centers (G14 and G24) or surrounds (G3 and G18), including one type, G6, that was double color-opponent (red-OFF/green-ON center:red-ON/green-OFF surround). Responses to colors were found primarily in sustained responses of bipolar and ganglion cells. However, in amacrine cells, transient components of the response also showed color dependence. Red-OFF-center responses were found in ganglion cells that were in a position to make connections at the strata 2/3 border with the red-OFF bipolar cell (B11); red-ON-center responses occurred in ganglion cells with branches in stratum 4 of the IPL where the red-ON-center bipolar (B10) ended. Blue-ON-center signals appeared to be processed mainly in strata 1-2/3, and blue-OFF-center signals in strata 3-5 of the IPL, with contributions of amacrine cells and bipolar cells. Labeled DS amacrine cells could be identified as A9, A20, and A22, and ganglion cells as G19, G20, and G24. The latter type (G24) showed DS and color coding. All response types (ON-center, OFF-center, ON-OFF) were encountered. DS amacrine cells were monostratified near the middle of the IPL, whereas DS ganglion cells were mono-, bi-, and multistratified, although all DS ganglion cells had one feature in common: they had dendrites in stratum 1 of the IPL.(ABSTRACT TRUNCATED AT 400 WORDS)

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“…The action potentials in turn may, or may not, contribute to transmitter release (Bieda and Copenhagen 1999;Watanabe et al 2000). Other populations of ACs are capable of producing membrane potential oscillations in response to light stimuli and/or in the dark (Djamgoz et al 1990; Sakai and Naka 1988;Teranishi et al 1987). The oscillations were thought to arise as a result of network activity.…”

Section: Introductionmentioning

confidence: 99%

Ionic Mechanisms Mediating Oscillatory Membrane Potentials in Wide-Field Retinal Amacrine Cells

Vı́gh

Solessio

Morgans³

et al. 2003

Journal of Neurophysiology

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Particular types of amacrine cells of the vertebrate retina show oscillatory membrane potentials (OMPs) in response to light stimulation. Historically it has been thought the oscillations arose as a result of circuit properties. In a previous study we found that in some amacrine cells, the ability to oscillate was an intrinsic property of the cell. Here we characterized the ionic mechanisms responsible for the oscillations in wide-field amacrine cells (WFACs) in an effort to better understand the functional properties of the cell. The OMPs were found to be calcium (Ca2+) dependent; blocking voltage-gated Ca2+ channels eliminated the oscillations, whereas elevating extracellular Ca2+ enhanced them. Strong intracellular Ca2+ buffering (10 mM EGTA or bis-( o-aminophenoxy)- N,N,N′ ,N′-tetraacetic acid) eliminated any attenuation in the OMPs as well as a Ca2+-dependent inactivation of the voltage-gated Ca2+ channels. Pharmacological and immunohistochemical characterization revealed that WFACs express L- and N-type voltage-sensitive Ca2+ channels. Block of the L-type channels eliminated the OMPs, but ω-conotoxin GVIA did not, suggesting a different function for the N-type channels. The L-type channels in WFACs are functionally coupled to a set of calcium-dependent potassium ( K(Ca)) channels to mediate OMPs. The initiation of OMPs depended on penitrem-A-sensitive (BK) K(Ca) channels, whereas their duration is under apamin-sensitive (SK) K(Ca) channel control. The Ca2+ current is essential to evoke the OMPs and triggering the K(Ca) currents, which here act as resonant currents, enhances the resonance as an amplifying current, influences the filtering characteristics of the cell membrane, and attenuates the OMPs via CDI of the L-type Ca2+ channel.

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Variability of light‐evoked response pattern and morphological characterization of amacrine cells in goldfish retina

Cited by 27 publications

References 42 publications

ON-OFF amacrine cells in cat retina

ON-OFF amacrine cells in cat retina

The organization of the turtle inner retina. II. Analysis of color‐coded and directionally selective cells

Ionic Mechanisms Mediating Oscillatory Membrane Potentials in Wide-Field Retinal Amacrine Cells

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