Variability of Kinetoplast DNA Gene Signatures of
            <i>Trypanosoma cruzi</i>
            II Strains from Patients with Different Clinical Forms of Chagas' Disease in Brazil

Lages‐Silva, Eliane; Ramı́rez, Luı́s Eduardo; Pedrosa, André Luiz; Crema, Eduardo; Galvão, Lúcia Maria da Cunha; Pena, Sérgio Danilo Junho; Macedo, Andréa Mara; Chiari, Égler

doi:10.1128/jcm.02124-05

Cited by 51 publications

(53 citation statements)

References 36 publications

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“…Importantly, our data were consistent with previous studies showing that DTU II was associated with human infection in the State of Minas Gerais 32,57,58 . The methodologies applied for genetic characterization of isolates of different clinical forms may be a limiting factor for typing T. cruzi populations since the analysis was based on a single marker, leading to potential misinterpretations 59 .…”

Section: Discussionsupporting

confidence: 83%

“…In contrast, it was not possible to correlate the parasite genotype with the clinical form. Findings using lowstringency single specific primer PCR and rDNA 24Sα markers showed TcII in all isolates from patients with indeterminate, cardiac, digestive, and cardiodigestive forms 58 . Although TcII was identified in most T. cruzi isolates, no correlation with clinical forms was observed using rDNA 24Sα, COII, and SL-IR markers and polymorphisms in microsatellite analysis 32 .…”

Section: Discussionmentioning

confidence: 99%

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Combined parasitological and molecular-based diagnostic tools improve the detection of Trypanosoma cruzi in single peripheral blood samples from patients with Chagas disease

Volpato

Sousa

D’Ávila

et al. 2017

Rev. Soc. Bras. Med. Trop.

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Introduction:In order to detect Trypanosoma cruzi and determine the genetic profiles of the parasite during the chronic phase of Chagas disease (ChD), parasitological and molecular diagnostic methods were used to assess the blood of 91 patients without specific prior treatment. Methods: Blood samples were collected from 68 patients with cardiac ChD and 23 patients with an indeterminate form of ChD, followed by evaluation using blood culture and polymerase chain reaction. T. cruzi isolates were genotyped using three different genetic markers. Results: Blood culture was positive in 54.9% of all patients, among which 60.3% had the cardiac form of ChD, and 39.1% the indeterminate form of ChD. There were no significant differences in blood culture positivity among patients with cardiac and indeterminate forms. Additionally, patient age and clinical forms did not influence blood culture results. Polymerase chain reaction (PCR) was positive in 98.9% of patients, although comparisons between blood culture and PCR results showed that the two techniques did not agree. Forty-two T. cruzi stocks were isolated, and TcII was detected in 95.2% of isolates. Additionally, one isolate corresponded to TcIII or TcIV, and another corresponded to TcV or TcVI. Conclusions: Blood culture and PCR were both effective for identifying T. cruzi using a single blood sample, and their association did not improve parasite detection. However, we were not able to establish an association between the clinical form of ChD and the genetic profile of the parasite.

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Section: Discussionsupporting

confidence: 83%

Section: Discussionmentioning

confidence: 99%

Combined parasitological and molecular-based diagnostic tools improve the detection of Trypanosoma cruzi in single peripheral blood samples from patients with Chagas disease

Volpato

Sousa

D’Ávila

et al. 2017

Rev. Soc. Bras. Med. Trop.

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“…It has been shown, in experimental as well as human T. cruzi infection, that parasites isolated from different organs are genetically distinct, suggesting that the genetic variability of the parasite strains may influence disease outcome [15,16]. However, a recent study has shown a high variability of T. cruzi kDNA signatures amongst isolates from patients with the same clinical form [17].…”

Section: Theories For the Generation And Maintenance Of (Immuno) Pathmentioning

confidence: 93%

Current concepts in immunoregulation and pathology of human Chagas disease

Dutra¹,

Gollob

2008

Current Opinion in Infectious Diseases

113

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Purpose of review-Chagas disease is a complex ailment caused by infection with Trypanosoma cruzi. It afflicts millions in Latin America. Years of studies have focused on the development of pathology in Chagas disease and recent studies have helped us understand the cellular mechanisms behind differential clinical evolution of Chagas disease.Recent findings-We discuss recent findings concerning the cellular immune response in human Chagas disease focusing on immunoregulation and the development of pathology. We seek to put several findings into the context of a disease that initially controls an extreme and patent infection, and later progresses to a chronic phase marked by the presence (cardiac and digestive forms), or not (indeterminate form), of associated pathology.Summary-Several theories exist to explain differential clinical evolution of Chagas disease. A coherent understanding of these theories will certainly aid in determining what combination of them approximates the true development of chagasic pathology. For achieving the goal of developing a successful therapy or intervention, it is critical that no theory be excluded at this point, but. Rather, rather that a thoughtful analysis and assimilation of the best components of each system into a central theory that best fits the reality of human Chagas disease is desirable.

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“…Por outro lado, o grupo T. cruzi I foi descrito em humanos na fase aguda e crônica da doença de Chagas em países da América Central, do norte da América do Sul e na região Amazônica brasileira (AÑEZ et al, 2004;TEIXEIRA et al, 2006 LAMOUR et al, 2005). Deve-se ressaltar que T. cruzi (mas não Leishmania e T. brucei) expressa uma prolina racemase que permite utilizar, além de L-prolina, D-prolina (CHAMOND et al, 2003;ATWOOD et al, 2005).…”

Section: Variabilidade Genética Das Populações De T Cruziunclassified

“…As formas epimastigotas de T. cruzi, cultivadas em meios com glicose e aminoácidos, consomem preferencialmente glicose (CAZZULO et al, 1985;LAMOUR et al, 2005 (NGO et al, 1998;WANG et al, 2000) e, recentemente, foi também aplicada em L. braziliensis (PEACOCK et al, 2007). Infelizmente, tanto T. cruzi como outras espécies de et al, 2004) não possuem a maquinaria de RNAi.…”

Section: Variabilidade Genética Das Populações De T Cruziunclassified

NADH desidrogenase mitocondrial de Trypanosoma cruzi: subunidade 7 para diagnóstico diferencial de isolados humanos e análise funcional.

Martínez¹

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Variability of Kinetoplast DNA Gene Signatures of Trypanosoma cruzi II Strains from Patients with Different Clinical Forms of Chagas' Disease in Brazil

Cited by 51 publications

References 36 publications

Combined parasitological and molecular-based diagnostic tools improve the detection of Trypanosoma cruzi in single peripheral blood samples from patients with Chagas disease

Combined parasitological and molecular-based diagnostic tools improve the detection of Trypanosoma cruzi in single peripheral blood samples from patients with Chagas disease

Current concepts in immunoregulation and pathology of human Chagas disease

NADH desidrogenase mitocondrial de Trypanosoma cruzi: subunidade 7 para diagnóstico diferencial de isolados humanos e análise funcional.

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