Variability of in vitro germination of Picea omorika pollen

Batos, Branislava; Nikolić, Biljana

doi:10.12657/denbio.069.002

Cited by 9 publications

(4 citation statements)

References 22 publications

(34 reference statements)

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“…In our previous investigations germination of P. omorika pollen was 54-68% (max. 94%) (Batos and Nikolić, 2013). In case of P. menziesii, the average values of pollen size were similar to those reported in the literature (Ho, 1968).…”

Section: Discussionsupporting

confidence: 88%

Needle Morpho-Anatomy and Pollen Morphophysiology of Selected Conifers in Urban Conditions

Nikolić¹,

Stefanović²,

Veselinović³

et al. 2019

Appl. Ecol. Env. Res.

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Comparison of twelve conifer species (Abies alba, A. concolor, A. nordmanniana, A. pinsapo, Cedrus atlantica, C. deodara, Picea abies, P. omorika, P. pungens, Pseudotsuga menziesii, Taxus baccata, and Pinus nigra) in the sense of needle morpho-anatomy and pollen morpho-physiology, in correlation to air pollution, was performed for the first time. Analyzed properties of species were also compared with literature sources. Listed conifers were investigated in five Belgrade parks, characterised by different degrees of air pollution, especially CO2. Their rank, I-V, was performed from non-polluted to heavily-polluted parks. Ranking in the sense of needle morpho-anatomy and pollen morpho-physiology did not match expected ones, but park V remained the worst for many analyzed species. Trees with shorter needles had greater stomatal density, which was particularly prominent in A. alba, A. nordmanniana, P. abies, P. omorika, P. nigra and T. baccata. The pollen grains of C. atlantica and T. baccata were the most sensitive to air pollution. In some analyzed species distance of particular trees close to the heavy traffic also was in correlation with needle dimensions (P.

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Section: Discussionsupporting

confidence: 88%

Needle Morpho-Anatomy and Pollen Morphophysiology of Selected Conifers in Urban Conditions

Nikolić¹,

Stefanović²,

Veselinović³

et al. 2019

Appl. Ecol. Env. Res.

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“…Whereas, the pollen which were stored for one week revealed lower pollen viability with 47.29% for safranin test and 33.14% for iodine test (Figure 3). Viability of pollen is extremely important for sexual reproduction of plants as reproduction success largely depends on the pollen dispersal and effective pollination within and between populations [36,37,38,39]. However, pollen viability has been reported to be affected by several factors.…”

Section: Pollen Viabilitymentioning

confidence: 99%

In vitro Studies on Pollen Viability, Pollen Germination and Pollen Tube Growth of Hornstedtia conoidea Ridl. – a Philippine Endemic Ginger Species

Mendez

Acma²

2018

J.Trop.Life.Science

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Zingiberaceous plants are predominantly propagated through underground rhizomes and seeds. In this study, the viability of pollen, rate of pollen germination and length of pollen tube of the Philippine endemic Hornstedtia conoidea were examined. Four petri plates containing pollen samples were prepared, of which two petri plates were used immediately after the collection, while the other two petri plates were stored for one week. The determination of pollen viability was made employing stain tests using IKI (iodine + potassium iodide) solution and safranin and measured immediately after the collection and after one week. Likewise, pollen germination test was carried out in vitro on agar medium and measured after 24 hours and one week of incubation. Data revealed that pollen viability was higher in the samples which were sown immediately with safranin with percentage pollen viability of 92.43%, while IKI test was lower with 89.36%. On the other hand, the pollen stored for one week measured 47.29% for safranin test and 33.14% for IKI test. The percentage germination of pollen after 24 hours was 72.65%, while 11.13% after one week. The pollen samples which were subjected for tube growth were examined for two weeks and gave positive results. Overall, it can be concluded that H. conoidea pollen can successfully be collected and stored for certain time. Knowledge regarding the pollen viability, pollen germination and pollen tube growth of this species will give practical benefit for plant breeding and conservation purposes, since H. conoidea is found in the wild, and as of now, the species has been recorded only in the Philippines.

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“…The samples with less than 5% of germinated grains were treated as samples with no germination. Measurements were done using a Leica Galen III light microscope (magnification x100) [36,37], with a camera (CCD Camera Topica TP/5001).…”

Section: Experimental Procedures and Determination Of Pollen Charactermentioning

confidence: 99%

Pollen viability in Quercus robur L.

Batos

2017

Arch biol sci (Beogr)

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The variability of viability (germination rate and the length of pollen tubes) of fresh pedunculate oak (Quercus robur L.) pollen grains was studied in vitro on a medium containing 15% sucrose. Spatial variability was studied by sampling fresh pollen grains from a total of thirteen trees at four different sites in the area of Belgrade (Kosutnjak, Banovo Brdo, Ada Ciganlija and Bojcin Forest) in a single year (2011). In order to assess temporal variability and determine the effects of climate change on a small time scale, we studied the viability of the pollen grains collected from one tree at the Banovo Brdo site in six different years (2004, 2005, 2006, 2007, 2011 and 2012). Interindividual variability was tested on the pollen grains sampled from eight trees at Ada Ciganlija in 2004. The percentage values of the pollen grain germination rate and the pollen tube length showed no statistically significant differences between the sites. However, the studied characteristics of the pollen grain viability (germination rate and pollen tube length) showed statistically significant differences in both temporal (between the pollen collection years) and interindividual variability. This type of research makes a valuable contribution to pedunculate oak breeding programs through the identification of trees with stable production and a good quality of pollen. Furthermore, it can be important in defining the patterns of spatial, temporal and individual variability of pollen grain viability under the influence of climate factors, which are showing compelling changing trends from year to year.

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Variability of in vitro germination of Picea omorika pollen

Cited by 9 publications

References 22 publications

Needle Morpho-Anatomy and Pollen Morphophysiology of Selected Conifers in Urban Conditions

Needle Morpho-Anatomy and Pollen Morphophysiology of Selected Conifers in Urban Conditions

In vitro Studies on Pollen Viability, Pollen Germination and Pollen Tube Growth of Hornstedtia conoidea Ridl. – a Philippine Endemic Ginger Species

Pollen viability in Quercus robur L.

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