Variability of Aggressiveness and Virulence of Erwinia carotovora subsp. Carotovorum Causing the Soft Rot on Potato Tubers in the Western of Algeria

Benada, M’hamed; Boumaaza, Boualem; Boudalia, Sofiane; Khaladi, Omar; Guessas, Bettache

doi:10.4081/pb.2018.7568

Cited by 7 publications

(7 citation statements)

References 23 publications

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“…Another species, E. carotovora , causes bacterial soft rot in economically important crops, such as potatoes, tomatoes, and cucumbers. In the case of potatoes, the soft rot of the stem and tubers occurs even after harvest, thus considerably reducing the yield [ 51 ]. Xanthomonas campestris pv.…”

Section: Discussionmentioning

confidence: 99%

Chemical Composition and Assessment of Antimicrobial Activity of Lavender Essential Oil and Some By-Products

Ciocârlan

Lupascu

Aricu

et al. 2021

Plants

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The producers of essential oils from the Republic of Moldova care about the quality of their products and at the same time, try to capitalize on the waste from processing. The purpose of the present study was to analyze the chemical composition of lavender (Lavanda angustifolia L.) essential oil and some by-products derived from its production (residual water, residual herbs), as well as to assess their “in vitro” antimicrobial activity. The gas chromatography-mass spectrometry analysis of essential oils produced by seven industrial manufacturers led to the identification of 41 constituents that meant 96.80–99.79% of the total. The main constituents are monoterpenes (84.08–92.55%), followed by sesquiterpenes (3.30–13.45%), and some aliphatic compounds (1.42–3.90%). The high-performance liquid chromatography analysis allowed the quantification of known triterpenes, ursolic, and oleanolic acids, in freshly dried lavender plants and in the residual by-products after hydrodistillation of the essential oil. The lavender essential oil showed good antibacterial activity against Bacillus subtilis, Pseudomonas fluorescens, Xanthomonas campestris, Erwinia carotovora at 300 μg/mL concentration, and Erwinia amylovora, Candida utilis at 150 μg/mL concentration, respectively. Lavender plant material but also the residual water and ethanolic extracts from the solid waste residue showed high antimicrobial activity against Aspergillus niger, Alternaria alternata, Penicillium chrysogenum, Bacillus sp., and Pseudomonas aeroginosa strains, at 0.75–6.0 μg/mL, 0.08–0.125 μg/mL, and 0.05–4.0 μg/mL, respectively.

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Section: Discussionmentioning

confidence: 99%

Chemical Composition and Assessment of Antimicrobial Activity of Lavender Essential Oil and Some By-Products

Ciocârlan

Lupascu

Aricu

et al. 2021

Plants

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“…Potato tubers showing typical symptoms were chosen. The infected parts were characterized by creamy to dark brown, granular consistency, spongy appearance (Benada et al, 2018) and releasing characteristic disagreeable odor (Rosenzweig et al, 2016).…”

Section: Methodsmentioning

confidence: 99%

“…In numerous potato cultivars in western Algeria, soft rot symptoms were reported in potato plants (Benada et al, 2018). When potato resistance is compromised, bacteria tend to cause infections by acting as opportunistic agents.…”

Section: Introductionmentioning

confidence: 99%

RAPID SCREENING OF PHYTOPATHOGENIC Erwinia sp. OF TWO POTATO VARIETIES (SPUNTA AND DESIREE) FROM ALGERIAN AGRICULTURAL FIELDS

Mokrani

Nabti

2021

JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA

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Rapid screening of phytopathogenic Erwinia sp. of two potato varieties (Spunta and Desiree) from Algerian agricultural fileds. Isolation, phenotypic identification and in vitro phytopathogenicity screening of Erwinia sp. from agricultural field of two potato varieties (Spunta and Desiree) in Algeria. The current study aims to isolate, identify and screen phytopathogenic isolates of Erwinia sp. causing potato diseases. The techniques presented in this study for isolation and characterization of phytopathogenic Erwinia sp. are conventional methods that are used in this field of research. Seven phytopathogenic bacteria were recovered from potato tubers of two varieties (Spunta and Desiree). The phenotypic identification allowed characterizing typical colonies of Erwinia sp. on two semi-selective media: King’s B and TCC media. Erwinia sp. formed characteristic colonies on King’s B medium that were round, convex and representing creamy color. While, Erwinia sp. also developed specific colonies on TCC medium which were pale purple, circular, convex, even bulging; smooth and mucous. In vitro phytopathogenicity test on potato slices lead to screen the phytopathogenic isolate E5 characterized by highest rotten tissue zone of (2.33 ± 0.29 cm) and (2.33 ± 0.58 cm) toward Spunta and Desiree varieties, respectively. Followed, by isolate E4 characterized by rotten tissue zone of (1.83 ± 0.58 cm) and (2.17 ± 0.29 cm) toward Spunta and Desiree varieties, respectively; compared to their corresponding uninfected controls. The RTZ (Rotten tissue zone) evidently is proportional to the specific pathogenicity of Erwinia sp. isolates and the characteristic sensitivity of various varieties (Spunta and Desiree). Thus, make determining RTZ a rapid screening technique for the selection of the highest phytopathogenic isolates. This investigation provides valuable information for rapid screening (infected potato tuber) and characterization (isolation using semi-selective media) of pathogenic Erwinia sp. engendering potato disease compared to existing methods like infection of leaves or plants; and phytopathogenic Erwinia sp. identification through PCR amplification or in situ hybridation.

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“…Potatoes from Spunta cultivar were used according to the method of Benada et al (2018), with some modifications; the potato tubers were first cleaned with water and surface disinfected by soaking them in 70% ethanol and briefly passing under the flame of a Bunsen burner. Sliced cuts were made, and then, wells in each slice were made (4 wells per slice of about 2-mm diameter and 5-mm depth).…”

Section: Bioassays On Potato Slicementioning

confidence: 99%

“…and Pectobacterium spp. Induced blackleg symptoms range from wet to dry stem rot depending on climatic conditions, whereas tubers can be affected by soft rots in the field and in storage (Benada et al 2018). Soft rot of potatoes has been caused by a range of bacteria around the world such as P. carotovorum subspecies carotovorum, P. atrosepticum, and Dickeya species.…”

Section: Introductionmentioning

confidence: 99%

Isolation and efficacy of two bacterial strains antagonists of Erwinia amylovora and Pectobacterium carotovorum

Benada

Boumaaza

Boudalia

et al. 2021

Egypt J Biol Pest Control

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Background The development of ecofriendly tools against plant diseases is an important issue in crop protection. Screening and selection process of bacterial strains antagonists of 2 pathogenic bacterial species that limit very important crops, Erwinia amylovora, the causal agent of the fire blight disease, and Pectobacterium carotovorum, the causal agent of bacterial potato soft rot, were reported. Bacterial colonies were isolated from different ecological niches, where both pathogens were found: rhizosphere of potato tubers and fruits and leaves of pear trees from the northwest region of Algeria. Direct and indirect confrontation tests against strains of E. amylovora and P. carotovorum were performed. Results Results showed a significant antagonistic activity against both phytopathogenic species, using direct confrontation method and supernatants of cultures (p<0.005). In vitro assays showed growth inhibitions of both phytopathogenic species. Furthermore, results revealed that the strains of S. plymuthica had a better inhibitory effect than the strains of P. fluorescens against both pathogens. In vivo results on immature pear fruits showed a significant decrease in the progression of the fire blight symptoms, with a variation in the infection index from one antagonistic strain to another between 31.3 and 50%, and slice of potato showed total inhibition of the pathogen (P. carotovorum) by the antagonistic strains of Serratia plymuthica (p<0.005). Conclusion This study highlighted that the effective bacteria did not show any infection signs towards plant tissue, and considered as a potential strategy to limit the fire blight and soft rot diseases.

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Variability of Aggressiveness and Virulence of Erwinia carotovora subsp. Carotovorum Causing the Soft Rot on Potato Tubers in the Western of Algeria

Cited by 7 publications

References 23 publications

Chemical Composition and Assessment of Antimicrobial Activity of Lavender Essential Oil and Some By-Products

Chemical Composition and Assessment of Antimicrobial Activity of Lavender Essential Oil and Some By-Products

RAPID SCREENING OF PHYTOPATHOGENIC Erwinia sp. OF TWO POTATO VARIETIES (SPUNTA AND DESIREE) FROM ALGERIAN AGRICULTURAL FIELDS

Isolation and efficacy of two bacterial strains antagonists of Erwinia amylovora and Pectobacterium carotovorum

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