Variability in the tandem repetitive DNA sequences of white spot syndrome virus (WSSV) genome and suitability of VP28 gene to detect different isolates of WSSV from India

Musthaq, S. Syed; Sudhakaran, R.; Ahmed, V.P. Ishaq; Balasubramanian, G.; Hameed, A.S. Sahul

doi:10.1016/j.aquaculture.2006.01.036

Cited by 39 publications

(42 citation statements)

References 26 publications

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“…Immunogold labeling has confirmed the presence of VP28 in the envelope (Zhang et al 2002). The neutralization of VP28 by antiserum protects shrimp from WSD; thus, VP28 plays an important role in infection (van Hulten et al 2001b, Syed Musthaq et al 2006. For this reason, we considered VP28 to be a good target for the study of the interaction of WSSV with the challenged freshwater prawn Macrobrachium rosenbergii.…”

Section: Discussionmentioning

confidence: 99%

Tolerance to white spot syndrome virus (WSSV) in the freshwater prawn Macrobrachium rosenbergii is associated with low VP28 envelope protein expression

Yoganandhan

Hameed²

2007

Dis. Aquat. Org.

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Section: Discussionmentioning

confidence: 99%

Tolerance to white spot syndrome virus (WSSV) in the freshwater prawn Macrobrachium rosenbergii is associated with low VP28 envelope protein expression

Yoganandhan

Hameed²

2007

Dis. Aquat. Org.

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“…1B−E, Table 1). ORF94 and ORF125 VNTR types have been used widely to distinguish WSSV strains (Wongteerasupaya et al 2003, Hoa et al 2005, 2011, and many WSSV strains have been associated with disease at shrimp farms in India (Syed Musthaq et al 2006, Pradeep et al 2008, John et al 2010, Walker et al 2011a. The VNTR variations detected were thus not unexpected considering the different species examined and the fact that the diseased shrimp had been sourced from different farms in different regions of South India.…”

Section: Genotyping Of Wssv Strainsmentioning

confidence: 99%

White spot syndrome virus (WSSV) genome stability maintained over six passages through three different penaeid shrimp species

Sindhupriya¹,

Saravanan²,

Otta³

et al. 2014

Dis. Aquat. Org.

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White spot syndrome virus (WSSV) replicates rapidly, can be extremely pathogenic and is a common cause of mass mortality in cultured shrimp. Variable number tandem repeat (VNTR) sequences present in the open reading frame (ORF)94, ORF125 and ORF75 regions of the WSSV genome have been used widely as genetic markers in epidemiological studies. However, reports that VNTRs might evolve rapidly following even a single transmission through penaeid shrimp or other crustacean hosts have created confusion as to how VNTR data is interpreted. To examine VNTR stability again, 2 WSSV strains (PmTN4RU and LvAP11RU) with differing ORF94 tandem repeat numbers and slight differences in apparent virulence were passaged sequentially 6 times through black tiger shrimp Penaeus monodon, Indian white shrimp Feneropenaeus indicus or Pacific white leg shrimp Litopenaeus vannamei. PCR analyses to genotype the ORF94, ORF125 and ORF75 VNTRs did not identify any differences from either of the 2 parental WSSV strains after multiple passages through any of the shrimp species. These data were confirmed by sequence analysis and indicate that the stability of the genome regions containing these VNTRs is quite high at least for the WSSV strains, hosts and number of passages examined and that the VNTR sequences thus represent useful genetic markers for studying WSSV epidemiology.

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“…The present report is based on three minisatellites ORF 75, 94 and 125. Application of these methods for typing WSSV isolates has been reported widely from the shrimp growing countries in the world (Dieu et al, 2004;Hoa et al, 2012;Gonzalez-Galaviz et al, 2013) while similar studies are limited from India (Musthaq et al, 2006;Pradeep et al, 2008;John et al, 2010) and the present study is the first report of WSSV genotyping from the state of Gujarat.…”

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confidence: 92%

Genetic variations among white spot syndrome virus (WSSV) isolates from shrimp farms in Gujarat, north-west coast of India

Patil¹,

Solanki²,

Otta³

et al. 2017

Indian J. Fish.

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The present paper documents the molecular diagnosis and genotyping of white spot syndrome virus (WSSV) isolates collected from different shrimp farms of Gujarat, north-west coast of India. Preliminary diagnosis was done using single tube PCR and genotyping of isolates by analysis of variable number tandem repeat sequence (VNTRS) in minisatellites viz., ORF 94, ORF 125, ORF 75 and ORF 75 flank. Out of the 79 samples analysed, 32 (33.80%) were positive with low (54.17%), medium (8.33%) and high (37.50%) severity of infection. Genotyping, using ORF 94 revealed that repeat units (RUs) 4 and 11 were the most frequent ones (32%) while ORF 125 showed 5 different repeat types of which RU 5 was the most common (45.83%). Two genotypes were observed for ORF 75, one showed multiple repeats of two 45 bp, followed by a 57 bp, whereas the other showed two repeats that included 5 and 4 repeats of 45 bp. Sequence analysis of the 54 bp variable sequence of ORF 94 RU type 4 revealed single-nucleotide polymorphism (SNP) at 47 th base. Based on the results from ORF 94, dominance of RU types 11or 4 were recorded for the first time in India and one sample showed 2 bands of different sizes indicating the prevalence of two different genotypes infecting the same pond at a given time. Further, no correlation could be observed between the virus genotype and the severity of the mortality in the outbreaks. The results suggest the widespread occurrence of WSSV in the region and circulation of distinct virus genotypes.

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Variability in the tandem repetitive DNA sequences of white spot syndrome virus (WSSV) genome and suitability of VP28 gene to detect different isolates of WSSV from India

Cited by 39 publications

References 26 publications

Tolerance to white spot syndrome virus (WSSV) in the freshwater prawn Macrobrachium rosenbergii is associated with low VP28 envelope protein expression

Tolerance to white spot syndrome virus (WSSV) in the freshwater prawn Macrobrachium rosenbergii is associated with low VP28 envelope protein expression

White spot syndrome virus (WSSV) genome stability maintained over six passages through three different penaeid shrimp species

Genetic variations among white spot syndrome virus (WSSV) isolates from shrimp farms in Gujarat, north-west coast of India

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