Variability in the diagnosis of dysplasia in ulcerative colitis by dynamic telepathology

Abstract

Abstract. Telepathology (TP) is the practice of evaluating pathology cases by the digital transmission of diagnostic slides as either static pictures (static TP) or by a continuous flow of pictures from a robotic microscopy (dynamic TP). The diagnostic efficacy of dynamic TP-based consultation services has not been widely tested. Dysplasia arising in association with chronic ulcerative colitis (CUC) is, at present, the most important marker for an increased risk of malignancy in patients with this disease. However, the diagnosis of dysplasia suffers from a significant degree of intra-and interobserver variability which usually necessitates a second opinion prior to definitive treatment. Thus, it is often necessary to obtain expert consultation of potential dysplasia cases by dedicated gastrointestinal pathologists. The aim of this study was to evaluate the utility and interobserver variability of diagnosing dysplasia in CUC with the use of dynamic TP. Dynamic TP was used to evaluate digitalized images of 38 CUC cases with areas considered negative, indefinite, or positive for dysplasia (low or high grade) independently by seven pathologists. Subsequently, all cases were graded by each of the pathologists by light microscopic examination of the H&E-stained glass slides. The degree of intra-and interobserver variability was determined by Kappa statistics. Overall, there was a poor degree of interobserver agreement (K=0.32) among the seven pathologists after analysis of the cases by dynamic TP. The poorest level of agreement was in the indefinite and lowgrade dysplasia categories, whereas the highest level was in the negative and high-grade dysplasia categories. Grouping together several diagnostic categories (for instance: Indefinite and low, or low-and high-grade dysplasia) had no significant effect on the level of agreement. The degree of variability in interpretation of cases by microscopic slide analysis was similar (K=0.35). After reviewing all the cases by microscopic analysis of the glass slides, the diagnosis was changed in 51% of the observations; in the majority of these (61%), the grade of dysplasia was decreased. In summary, the use of dynamic TP for consultation in CUC-associated dysplasia has a poor level of interobserver agreement, but does not differ significantly from that obtained by the evaluation of the cases by microscopic slide analysis. Diagnoses rendered by dynamic TP tend to be of a higher grade compared to that obtained by microscopic slide analysis. Thus, although dynamic TP may be used for the consultation of CUC dysplasia cases, more specific criteria are needed in the general categorization of dysplasia in CUC.

Evaluation of whole slide image immunohistochemistry interpretation in challenging prostate needle biopsies

“…Previously reported studies describing the measurement of pathologist interobserver agreement when viewing other surgical pathology specimen types using conventional light microscopy or digital images (either static or dynamic) show highly variable levels of agreement, ranging from essentially perfect [19] to fair [20]. Regardless of the specific κ statistics calculated, measurements of interobserver agreement that are essentially no better for microscopic slides compared to digital images support the validity of digital images for use in telepathology-based consultation.…”

Endoscopy in the Diagnosis and Management of Complications of Inflammatory Bowel Disease

“…72,80 Because of low interobserver agreement regarding dysplasia, especially in the context of inflammation, 2 expert gastrointestinal pathologists should confirm dysplasia. 81 There is no pathognomonic appearance in IBD, making endoscopic surveillance difficult. DALMs may appear as a single polypoid mass, a plaque-like lesion or multiple polyps.…”

Medicolegal aspects of telepathology

“…For instance, Odze et al [18] have shown that, although there is no significant difference in the degree of interobserver variability in telepathology diagnosis of dysplasia in ulcerative colitis using fluid images over glass slides, when participants were given the glass slides to review, most of the expert gastrointestinal pathologists (51%) changed their diagnosis, and a majority of these cases (61%) downgraded the degree of dysplasia from the original telepathology diagnosis. The high interobserver variability is well documented in issues of gastrointestinal dysplasia.…”