Variability in the carbazole assay for N-desulfonated/N-acylated heparin derivatives

Pseudomonas aeruginosaMucD Regulates the Alginate Pathway through Activation of MucA Degradation via MucP Proteolytic Activity

“…The hydrolyzed sugar monomer is then reacted with the carbazole reagent for detection (9). However, some neutral sugars, such as hexoses and pentoses, as well as the acyl groups of uronic acids, can interfere with the specificity of the reaction (1,6,8). Furthermore, even DNA has been shown to affect this assay (21).…”