Variability in symbiotic effectiveness of N2 fixing bacteria in Mimosa scabrella

Primieri, Silmar; Costa, Murilo Dalla; Stroschein, Marcos Roberto Dobler; Stocco, Priscila; Santos, Júlio César Pires; Antunes, Pedro M.

doi:10.1016/j.apsoil.2016.01.018

Cited by 14 publications

(4 citation statements)

References 31 publications

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“…We identified the active bacterial communities (Figure S6), that is, symbiotic Symbiobacterium, iron-reducing Geobacter, denitrifying bacteria Rhodocyclaceae, and N-fixing Azospirillum, strongly raising ( p < 0.01) with HO • and Fe(II) increase. In contrast, N-fixing and iron acquiring Burkholderia, , denitrifier Xanthomonadaceae, N-fixing Rhizobiales, and Brevibacillus strongly decrease ( p < 0.01) with increasing HO • and Fe(II) concentrations. Although these nitrogen- and iron-cycling species have been extensively reported in soils, we provide the first evidence that the key microbial communities are linked to HO • formation and thus soil Fenton oxidation.…”

Section: Discussionmentioning

confidence: 91%

Molybdenum Bioavailability and Asymbiotic Nitrogen Fixation in Soils are Raised by Iron (Oxyhydr)oxide-Mediated Free Radical Production

Kuzyakov

Luo

et al. 2021

Environ. Sci. Technol.

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Nitrogen (N) fixation in soils is closely linked to microbially mediated molybdenum (Mo) cycling. Therefore, elucidating the mechanisms and factors that affect Mo bioavailability is crucial for understanding N fixation. Here, we demonstrate that long-term (26 years) manure fertilization increased microbial diversity and content of short-range ordered iron (oxyhydr)oxides that raised Mo bioavailability (by 2.8 times) and storage (by ∼30%) and increased the abundance of nif H genes (by ∼14%) and nitrogenase activity (by ∼60%). Nanosized iron (oxyhydr)oxides (ferrihydrite, goethite, and hematite nanoparticles) play a dual role in soil Mo cycling: (i) in concert with microorganisms, they raise Mo bioavailability by catalyzing hydroxyl radical (HO • ) production via the Fenton reactions and (ii) they increase Mo retention by association with the nanosized iron (oxyhydr)oxides. In summary, long-term manure fertilization raised the stock and bioavailability of Mo (and probably also of other micronutrients) by increasing iron (oxyhydr)oxide reactivity and intensified asymbiotic N fixation through an increased abundance of nif H genes and nitrogenase activity. This work provides a strategy for increasing biological N fixation in agricultural ecosystems.

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Section: Discussionmentioning

confidence: 91%

Molybdenum Bioavailability and Asymbiotic Nitrogen Fixation in Soils are Raised by Iron (Oxyhydr)oxide-Mediated Free Radical Production

Kuzyakov

Luo

et al. 2021

Environ. Sci. Technol.

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“…The height and diameter of plants have been recently used for evaluation of symbiotic efficiency of rhizobia in tree legumes (Ceccon et al, 2012;Ramos et al, 2013;Primieri et al, 2016;Menezes et al, 2017). These parameters indicate the quality of the seedlings, the potential of survival and growth after transplanting.…”

Section: Discussionmentioning

confidence: 99%

Symbiotic efficiency of native rhizobia in legume tree Leucaena leucocephala derived from several soil classes of Brazilian Northeast region

Silva¹,

Santos²,

Freitas³

et al. 2018

Aust J Crop Sci

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The effective symbiotic activity of rhizobia can contribute increment of biological nitrogen fixation (BNF), which is of great importance in low technology regions. This study aimed to evaluate the symbiotic efficiency of native rhizobia from Brazilian Northeast soils for selection of the most efficient and competitive strains for Leucaena leucocephala plant. The greenhouse test was conducted in factorial scheme (7x3) ). The plants were harvested at 90 days and the following characteristics were determined: plant height, diameter, shoots dry matter, roots dry matter and nodules dry matter, number of nodules, nitrogen concentration and accumulation in shoots, and efficiency of nitrogen fixation. The isolates 36F and 45G are very promising on Biologic Nitrogen Fixation (BNF). They showed high symbiotic efficiency (higher than 90%). These selected strains provided the best characteristics such as plant height (57 and 52 cm), diameter (4.03 and 4.02 mm), dry matter of shoot (3.2 and 3.6 g plant -1) and nitrogen accumulation in shoots (60 and 65 mg plant-1).

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“…Leaf material supports microbial growth 5 min, followed by 30 cycles of denaturing at 94°C for 30 s, annealing at 55°C for 30 s, extension at 72°C for 45 s, and a final extension at 72°C for 10 min. The phylogenetic analyses of bacteria were carried out by acquisition of 16S rDNA ribosomal gene sequence using PCR with the universal primers 27F (5′-AGAG TTTGATCCTGGCTCAG-3′) and 1500R (5′-GGTT ACCTTGTTACGACTT-3′) [13]. The PCR conditions were as follows: initial denaturing at 95°C for 4 min, followed by 30 cycles of denaturing at 94°C for 30 s, annealing at 50°C for 45 s, extension at 72°C for 60 s, and a final extension at 72°C for 8 min.…”

Section: Microbial Biodiversity Analysismentioning

confidence: 99%

Fungi as Architects of the Rimstone Dams in Huanglong, NSD, Sichuan, China

et al. 2016

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The Huanglong park area of the Sichuan Province of China is a unique scenic area of the world. It is known for its thousands of aquamarine-colored pools that are formed behind naturally formed rimstone dams of travertine (calcite) along a cold water stream. The travertine, based on its crystalline structural analysis, is of biological origin. This makes sense since the temperature of the waters of Huanglong varies from 5 to 7 °C and thus geochemical crystallization does not occur as it does in other locations around the world possessing thermal pools whose structures are primarily formed through cooling processes. Fungi and bacteria were discovered associated with both leaves associated with the calcite dams as well as in the older parts of well-established dams. Several species of Phytium, a phycomycete and an endophyte, accounted for over 45 % of all of the fungi successfully isolated from the well-established dam samples and at least 85 % in the floating leaf samples. Saprolegnia spp. (Phycomycetes) along with Phoma spp. (Ascomycetes) were noted along with Mortierella sp. as other dam-associated fungi. The fungal hyphae observed on dead leaf material as well as in the calcite dams directly served as nucleation points for the formation of crystalline CaCO. Eventually, these crystals grow large enough to fuse to make calcite plates which form the main structural feature of all of the travertine dams in this area. Interestingly, each of the individual crystals associated with the dams has an associated hole in its core where a fungal hypha used to reside as observed by scanning electron microscopy. While diatoms were present in the analysis, they too seem to contribute to the structure of the dams but in a minor way. The only bacteria isolated from the older dam of this aquatic environment were Pseudomonas spp. and their role in dam formation is uncertain. Huanglong is a unique and beautiful place, and the water features present in this area can definitely be attributed to those fungal architects that encourage calcite crystal formation.

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Variability in symbiotic effectiveness of N2 fixing bacteria in Mimosa scabrella

Cited by 14 publications

References 31 publications

Molybdenum Bioavailability and Asymbiotic Nitrogen Fixation in Soils are Raised by Iron (Oxyhydr)oxide-Mediated Free Radical Production

Molybdenum Bioavailability and Asymbiotic Nitrogen Fixation in Soils are Raised by Iron (Oxyhydr)oxide-Mediated Free Radical Production

Symbiotic efficiency of native rhizobia in legume tree Leucaena leucocephala derived from several soil classes of Brazilian Northeast region

Fungi as Architects of the Rimstone Dams in Huanglong, NSD, Sichuan, China

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