Variability in protein quality used for embryo culture: embryotoxicity of the stabilizer octanoic acid

Leonard, Phoebe H.; Charlesworth, M. Cristine; Benson, Linda M.; Walker, David; Fredrickson, J.R.; Morbeck, Dean E.

doi:10.1016/j.fertnstert.2013.03.034

Cited by 26 publications

(21 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, this is not the only potential contaminant that may be present in products used for embryo culture. A variety of other chemical contaminants have been detected in different culture supplies, including peroxides, alkenes, and octanoic acid [2,3,41]. Similarly, some components of the Our results indicate that a MEA utilizing IVM-derived embryos from SW mice is highly sensitive to TX100, since multiple endpoints detected low levels of this contaminant.…”

Section: Discussionmentioning

confidence: 51%

“…Even when the highest-quality reagents and plasticware are used, the introduction of some unknown substances (contaminants) into the culture environment is unavoidable [1][2][3]. As a result, all supplies that will come into contact with the embryo and/or the culture medium should be subjected to strict quality control (QC) testing, including the ability to support the growth of murine embryos [4,5].…”

Section: Introductionmentioning

confidence: 99%

“…product should be used for clinical ART. The more troubling scenario is the possibility that a product could support the development of murine embryos but still be unsuitable for clinical use [3,6]. The key to preventing such products from entering the clinic is to improve the sensitivity of MEAs and ensure clinically relevant results.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Building a better mouse embryo assay: effects of mouse strain and in vitro maturation on sensitivity to contaminants of the culture environment

Herrick

Paik

Strauss

et al. 2015

J Assist Reprod Genet

View full text Add to dashboard Cite

Purpose The aim of this study is to compare the sensitivity of the standard one-cell mouse embryo assay (MEA) to that using in vitro-matured oocytes from hybrid and outbred mice. Methods The study was done by culturing embryos in the presence or absence of two concentrations (0.0005 or 0.001 %v/v) of Triton X-100 (TX100). Embryonic development, blastocyst cell numbers (total and allocation to the trophectoderm [TE] and inner cell mass [ICM]), and blastocyst gene expression were evaluated. Results Neither concentration of TX100 affected (P>0.05) cleavage, blastocyst development, or hatching in one-cell embryos from BDF1 mice. However, all cell number endpoints were reduced (P<0.05) by the high concentration of TX100 and the number of ICM cells was reduced (P<0.05) by the low concentration of TX100. Inhibitory (P<0.05) effects of the high concentration of TX100 were observed in in vitro maturation (IVM) embryos from BDF1, CF1, and SW, but not ICR, mice. Cell number and allocation were negatively affected by the high concentration of TX100 in CF1 and SW embryos, but not in BDF1 or ICR embryos. The only developmental endpoints affected by the low concentration of TX100 were cleavage of BDF1 oocytes, blastocyst development of SW embryos, and cell numbers (total and inner cell mass (ICM)) of SW blastocysts. Conclusions The sensitivity of the MEA to TX100 is improved by using embryos from in vitro-matured oocytes, using oocytes from some outbred (SW or CF1, not ICR) strains of mice, and evaluating blastocyst cell number and allocation.

show abstract

Section: Discussionmentioning

confidence: 51%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Building a better mouse embryo assay: effects of mouse strain and in vitro maturation on sensitivity to contaminants of the culture environment

Herrick

Paik

Strauss

et al. 2015

J Assist Reprod Genet

View full text Add to dashboard Cite

show abstract

“…Protein is an important component of embryo culture with documented lot-to-lot variability [1][2][3][4][5][6][7] that can adversely affect embryo development [1,2,4,[8][9][10]. Protein supplements are poorly defined and thus represent a gap in our control of quality in the clinical embryology laboratory.…”

Section: Introductionmentioning

confidence: 99%

“…Albumin is also a carrier of hormones, growth factors, energy substrates (citrate and fatty acids), medications, enzymes, and metals [6]. In addition, stabilizers added to HSA preparations show batch variation at concentrations that affect mouse embryo development [4]. Complex protein supplements show even more variability than HSA.…”

Section: Introductionmentioning

confidence: 99%