Variability in APOE genotype status in human-derived cell lines: a cause for concern in cell culture studies?

Schaffer, Sebastian; Lam, Vanessa Yuk Man; Ernst, Insa M. A.; Huebbe, Patricia; Rimbach, Gerald; Halliwell, Barry

doi:10.1007/s12263-013-0364-4

Cited by 12 publications

(10 citation statements)

References 66 publications

(67 reference statements)

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“…The role of apolipoprotein E (APOE) in TTR knockdown mediated by Patisiran was explored in the hepatoma cell line HepG2. As previously reported [17], HepG2 cells express the APOE variant E3/E3. In order to characterise the role of APOE in Patisiran-mediated TTR knockdown, HepG2 cells were transfected with an siRNA directed against APOE.…”

Section: Apoe Expression Impacts Patisiran-mediated Ttr Knockdown In supporting

confidence: 78%

APOE polymorphism in ATTR amyloidosis patients treated with lipid nanoparticle siRNA

Niemietz

Nadzemova

Zibert

et al. 2019

Amyloid

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Section: Apoe Expression Impacts Patisiran-mediated Ttr Knockdown In supporting

confidence: 78%

APOE polymorphism in ATTR amyloidosis patients treated with lipid nanoparticle siRNA

Niemietz

Nadzemova

Zibert

et al. 2019

Amyloid

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“…Another cautionary note concerns the ApoE effects on cholesterol secretion. In this respect, Caco-2 cells possess the most commonly found ApoE3/E3 genotype and, therefore, our results should be applied to the general population. However, the intestines of genotypically different individuals might react differently to polyphenol exposure.…”

Section: Resultsmentioning

confidence: 98%

Green Tea, Cocoa, and Red Wine Polyphenols Moderately Modulate Intestinal Inflammation and Do Not Increase High-Density Lipoprotein (HDL) Production

Nicod

Chiva‐Blanch

Giordano

et al. 2014

J. Agric. Food Chem.

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Although polyphenols are often merely perceived as antioxidants, their biological activities are manifold and include anti-inflammatory actions. A new area of research on polyphenols and health concerns their putative role in cholesterol metabolism, in particular, their high-density lipoprotein-cholesterol (HDL-c)-raising potential. Indeed, some human studies showed that administration of polyphenol-rich foods such as cocoa, green tea, and extra virgin olive oil modulate and increase HDL-c concentrations. This study assessed the effects of polyphenols on intestinal inflammation, using the physiologically relevant Caco-2 Transwell model and using lipopolysaccharide (LPS) to trigger inflammation. This study also investigated the mechanisms of actions behind the proposed HDL-c-increasing effects of polyphenols. The data suggest that polyphenols (at least those from red wine, cocoa, and green tea) administered at a dietary dose moderately modulate intestinal inflammation but do not increase cholesterol secretion by intestinal cells or enhance HDL functionality. Nutraceuticals and supplements provide pharmanutritional doses that might, conversely, produce beneficial effects.

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“…This important gene is involved in metabolism of lipids and in regulating innate immune responses. There are three uniquely human ApoE alleles, of which ApoE e4 and ApoE e3 are the most prevalent (Schaffer et al, 2014 ). The ApoE e4 differs in several critical amino acids from the chimpanzee equivalent.…”

Section: Are Primate Studies Predictable For Humans?mentioning

confidence: 99%

Immunological and Technical Considerations in Application of Alginate-Based Microencapsulation Systems

Juárez¹,

Spasojević²,

Faas³

et al. 2014

Front. Bioeng. Biotechnol.

150

128

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Islets encapsulated in immunoprotective microcapsules are being proposed as an alternative for insulin therapy for treatment of type 1 diabetes. Many materials for producing microcapsules have been proposed but only alginate does currently qualify as ready for clinical application. However, many different alginate-based capsule systems do exist. A pitfall in the field is that these systems are applied without a targeted strategy with varying degrees of success as a consequence. In the current review, the different properties of alginate-based systems are reviewed in view of future application in humans. The use of allogeneic and xenogeneic islet sources are discussed with acknowledging the different degrees of immune protection the encapsulation system should supply. Also issues such as oxygen supply and the role of danger associated molecular patterns (DAMPS) in immune activation are being reviewed. A common property of the encapsulation systems is that alginates for medical application should have an extreme high degree of purity and lack pathogen-associated molecular patterns (PAMPs) to avoid activation of the recipient’s immune system. Up to now, non-inflammatory alginates are only produced on a lab-scale and are not yet commercially available. This is a major pitfall on the route to human application. Also the lack of predictive pre-clinical models is a burden. The principle differences between relevant innate and adaptive immune responses in humans and other species are reviewed. Especially, the extreme differences between the immune system of non-human primates and humans are cumbersome as non-human primates may not be predictive of the immune responses in humans, as opposed to the popular belief of regulatory agencies. Current insight is that although the technology is versatile major research efforts are required for identifying the mechanical, immunological, and physico-chemical requirements that alginate-based capsules should meet for successful human application.

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Variability in APOE genotype status in human-derived cell lines: a cause for concern in cell culture studies?

Cited by 12 publications

References 66 publications

APOE polymorphism in ATTR amyloidosis patients treated with lipid nanoparticle siRNA

APOE polymorphism in ATTR amyloidosis patients treated with lipid nanoparticle siRNA

Green Tea, Cocoa, and Red Wine Polyphenols Moderately Modulate Intestinal Inflammation and Do Not Increase High-Density Lipoprotein (HDL) Production

Immunological and Technical Considerations in Application of Alginate-Based Microencapsulation Systems

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