Variability in accumulation of free proline on in vitro calli of four bean (Phaseolus vulgaris L.) varieties exposed to salinity and induced moisture stress

Cárdenas-Ávila, ML; Verde-Star, Julia; Maiti, R K; Foroughbakhch-P, R; Gamez-Gonzalez, Hilda; Martinez-Lozano, Salomon Javier; Núñez-González, María Adriana; Díaz, G; Hernández-Piñero, J. L.; Morales-Vallarta,

doi:10.32604/phyton.2006.75.103

Cited by 7 publications

(5 citation statements)

References 7 publications

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“…However, there are some contradictory data in the literature regarding the function of Pro in the mechanisms of stress tolerance in Phaseolus. Some published reports indicated higher Pro contents in more drought-tolerant [30,[34][35][36][37] or salt-tolerant [31][32][33] cultivars than in less tolerant ones; that is, Pro accumulation correlates positively with the degree of stress resistance, suggesting a direct contribution to stress tolerance mechanisms. Other reports, on the contrary, showed that, under stress conditions, the less tolerant genotypes had a higher concentration of this osmolyte than the more resistant cultivars [38][39][40]66]; therefore, in this case, Pro is simply a marker of the level of stress affecting the plants, accumulating at higher concentrations in the more stressed-the more sensitive-cultivars, but is not directly involved in the mechanisms of tolerance.…”

Section: Discussionmentioning

confidence: 99%

“…However, it is not yet clear whether Pro accumulation in P. vulgaris is associated with enhanced or reduced tolerance to stress since contradictory results are available in the literature. Some reports correlated higher Pro contents with a relatively higher stress tolerance when comparing different bean cultivars [30][31][32][33][34][35][36][37], whereas in other cases higher Pro concentrations were measured in the relatively more stress-sensitive cultivars [38][39][40]. All these studies were based on the comparison of a few genotypes.…”

Section: Introductionmentioning

confidence: 99%

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The Use of Proline in Screening for Tolerance to Drought and Salinity in Common Bean (Phaseolus vulgaris L.) Genotypes

et al. 2020

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The selection of stress-resistant cultivars, to be used in breeding programmes aimed at enhancing the drought and salt tolerance of our major crops, is an urgent need for agriculture in a climate change scenario. In the present study, the responses to water deficit and salt stress treatments, regarding growth inhibition and leaf proline (Pro) contents, were analysed in 47 Phaseolus vulgaris genotypes of different origins. A two-way analysis of variance (ANOVA), Pearson moment correlations and principal component analyses (PCAs) were performed on all measured traits, to assess the general responses to stress of the investigated genotypes. For most analysed growth variables and Pro, the effects of cultivar, treatment and their interactions were highly significant (p < 0.001); the root morphological traits, stem diameter and the number of leaves were mostly due to uncontrolled variation, whereas the variation of fresh weight and water content of stems and leaves was clearly induced by stress. Under our experimental conditions, the average effects of salt stress on plant growth were relatively weaker than those of water deficit. In both cases, however, growth inhibition was mostly reflected in the stress-induced reduction of fresh weight and water contents of stems and leaves. Pro, on the other hand, was the only variable showing a negative correlation with all growth parameters, but particularly with those of stems and leaves mentioned above, as indicated by the Pearson correlation coefficients and the loading plots of the PCAs. Therefore, in common beans, higher stress-induced accumulation of Pro is unequivocally associated with a stronger inhibition of growth; that is, with a higher sensitivity to stress of the corresponding cultivar. We propose the use of Pro as a suitable biochemical marker for simple, rapid, large-scale screenings of bean genotypes, to exclude the most sensitive, those accumulating higher Pro concentrations in response to water or salt stress treatments.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The Use of Proline in Screening for Tolerance to Drought and Salinity in Common Bean (Phaseolus vulgaris L.) Genotypes

et al. 2020

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“…To defend against leaf water deficit. For example, plants may increase the synthesis of compatible solutes such as proline after being subjected to DS conditions [39][40][41]. In this study, when leaf RWC and ψ shoot decreased during DS treatment, C. paniculatum accumulated the highest level of proline ( Accumulating solutes decreases cell osmotic potential, which could be the main factor resulting in the drop of its ψ shoot [14].…”

Section: Changes In Leaf Proline Concentration During Ds and Rw Treat...mentioning

confidence: 56%

“…To defend against leaf water deficit. For example, plants may increase the synthesis of compatible solutes such as proline after being subjected to DS conditions [ 39 – 41 ]. In this study, when leaf RWC and ψ shoot decreased during DS treatment, C .…”

Section: Discussionmentioning

confidence: 99%

“…malabathricum was unable to engage this drought stress tolerant mechanism. Various studies align with the increase in proline levels in DS-treated plants [ 39 , 40 ], and large proline accumulation is usually associated with DS tolerance [ 41 ]. It was reported that proline has antioxidant and osmotic properties that improve the drought tolerance of oilseed rape [ 42 ].…”

Section: Discussionmentioning

confidence: 99%

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Photosynthetic gas exchange, plant water relations and osmotic adjustment of three tropical perennials during drought stress and re-watering

He,

Ng,

Qin

et al. 2024

PLoS ONE

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Planting vegetation on slopes is an effective way of improving slope stability while enhancing the aesthetic appearance of the landscape. However, plants growing on slopes are susceptible to natural drought stress (DS) conditions which commonly lead to water deficit in plant tissues that affect plant health and growth. This study investigated the photosynthetic gas exchange, plant water status and proline accumulation of three tropical perennials namely Clerodendrum paniculatum, Ipomoea pes-caprae and Melastoma malabathricum after being subjected to DS and re-watering (RW). During DS, there was a significant decrease in light-saturated photosynthetic CO2 assimilation rate (Asat), stomatal conductance (gs sat), and transpiration rate (Tr) for all three plant species. Leaf relative water content, shoot water potential, and leaf, stem and root water content also declined during DS. Proline concentration increased for all three species during DS, reaching especially high levels for C. paniculatum, suggesting that it heavily relies on the accumulation of proline to cope with DS. Most of the parameters recovered almost completely to levels similar to well-watered plants after RW, apart from M. malabathricum. Strong linear correlations were found between Asat and gs sat and between gs sat and Tr. Ultimately, C. paniculatum and I. pes-caprae had better drought tolerance than M. malabathricum.

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Biochemical and Molecular Markers Associated With Salinity Tolerance in Bread Wheat Genotypes (Triticum Aestivum L.) Under Saline Conditions

El-Saber

2021

Egyptian Journal of Desert Research

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his investigation was applied to find out the seven bread wheat lines that are more tolerant to saline stress (6195 ppm soil and 8934 ppm irrigated water) at Ras Sudr, South Sinai, Egypt. Data indicated that bread wheat lines greatly varied in their ability to grow and subsequently assimilate and translocate biochemical components. Line1 (L1) surpassed the other lines, it was recorded the maximum values of growth, yield, and proline as well as the lowest malondialdehyde content followed by L3 and L7. ISSR-PCR analysis was used for discriminating between lines. A total of 102 DNA bands are detected across the six ISSR primers, out of which only 72 bands (51 non-unique bands and 21 unique bands) are polymorphic with polymorphism percentages (70.58%). In this regard, primer ISSR2 gave the highest polymorphism (92%) followed by ISSR-13 (86%) and ISSR-11 (78%). Also, patterns of proteins (SDS-PAGE) and superoxide dismutase (SOD) have been successfully used in discriminating between salt-tolerant and saltsensitive lines. Concerning SDS-PAGE, bands number in 7 wheat lines ranged from 13 to 14 with molecular weights ranging between 6 to 130 kDa, and the more intensive bands are presented at 25, 29, and 34 kDa. Moreover, SOD patterns indicated the presence of 5 bands; the major is presented at bands (No. 4 and 5) in all wheat lines. Based on the results obtained, L1 was the best wheat line followed by L3 and L7 which more tolerant to saline stress, and this was associated with increasing growth, yield, and proline as well as decreasing MDA content. In conclusion, SDS-PAGE, SOD, and ISSR analyses are useful biochemical and molecular tools to discriminate different bread wheat lines under saline conditions.

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Variability in accumulation of free proline on in vitro calli of four bean (Phaseolus vulgaris L.) varieties exposed to salinity and induced moisture stress

Cited by 7 publications

References 7 publications

The Use of Proline in Screening for Tolerance to Drought and Salinity in Common Bean (Phaseolus vulgaris L.) Genotypes

The Use of Proline in Screening for Tolerance to Drought and Salinity in Common Bean (Phaseolus vulgaris L.) Genotypes

Photosynthetic gas exchange, plant water relations and osmotic adjustment of three tropical perennials during drought stress and re-watering

Biochemical and Molecular Markers Associated With Salinity Tolerance in Bread Wheat Genotypes (Triticum Aestivum L.) Under Saline Conditions

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