Variability for Seed-based Economic Traits and Genetic Diversity Analysis in Mucuna pruriens Population of Northeast India

Lepcha, Patrush; Sathyanarayana, N.

doi:10.1007/s40003-021-00568-6

Cited by 4 publications

(1 citation statement)

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“…Genetic differentiation is an important index of the genetic structure of populations within a species ( Wang, 2020 ). In the current study, gene flow of E. stauntonii ( N m = 1.252) was moderately low, lower than that reported for Eragrostis tef ( N m = 4.742) ( Tadesse et al, 2021 ) and Mucuna pruriens ( N m = 7.48) ( Lepcha and Sathyanarayana, 2021 ), but it was consistent with the high genetic differentiation ( F st = 0.181) ( Balloux and Lugon-Moulin, 2002 ). The low gene flow, which might be caused by the small sampling range and habitat fragmentation, increased the within-population genetic variation and promoted population differentiation ( Young et al, 1996 ; Li et al, 2014 ; Peng et al, 2021 ).…”

Section: Discussionsupporting

confidence: 67%

Genetic Diversity Study on Geographical Populations of the Multipurpose Species Elsholtzia stauntonii Using Transferable Microsatellite Markers

et al. 2022

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Elsholtzia stauntonii Benth. (Lamiaceae) is an economically important ornamental, medicinal and aromatic plant species. To meet the increasing market demand for E. stauntonii, it is necessary to assess genetic diversity within the species to accelerate the process of genetic improvement. Analysis of the transferability of simple sequence repeat (SSR) markers from related species or genera is a fast and economical method to evaluate diversity, and can ensure the availability of molecular markers in crops with limited genomic resources. In this study, the cross-genera transferability of 497 SSR markers selected from other members of the Lamiaceae (Salvia L., Perilla L., Mentha L., Hyptis Jacq., Leonurus L., Pogostemon Desf., Rosmarinus L., and Scutella L.) to E. stauntonii was 9.05% (45 primers). Among the 45 transferable markers, 10 markers revealed relatively high polymorphism in E. stauntonii. The genetic variation among 825 individuals from 18 natural populations of E. stauntonii in Hebei Province of China was analyzed using the 10 polymorphic SSR markers. On the basis of the SSR data, the average number of alleles (NA), expected heterozygosity (HE), and Shannon’s information index (I) of the 10 primers pairs were 7.000, 0.478, and 0.688, respectively. Lower gene flow (Nm = 1.252) and high genetic differentiation (Fst = 0.181) were detected in the populations. Analysis of molecular variance (AMOVA) revealed that most of the variation (81.47%) was within the populations. Integrating the results of STRUCTURE, UPGMA (Unweighted Pair Group Method with Arithmetic Mean) clustering, and principal coordinate analysis, the 825 samples were grouped into two clusters associated with geographical provenance (southwestern and northeastern regions), which was consistent with the results of a Mantel test (r = 0.56, p < 0.001). Overall, SSR markers developed in related genera were effective to study the genetic structure and genetic diversity in geographical populations of E. stauntonii. The results provide a theoretical basis for conservation of genetic resources, genetic improvement, and construction of a core collection for E. stauntonii.

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Section: Discussionsupporting

confidence: 67%

Genetic Diversity Study on Geographical Populations of the Multipurpose Species Elsholtzia stauntonii Using Transferable Microsatellite Markers

et al. 2022

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Bayesian Inference of Soil Traits from Green Manure Fields in a Tropical Sandy Soil

dos Santos Nascimento,

Souza,

da Silva

et al. 2024

Int. J. Plant Prod.

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Genetic variability and divergence studies on seed traits and L-dopa content of Mucuna pruriens (L.) DC. accessions

Patel

Azeez

Krishnamurthy

2023

Preprint

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Mucuna pruriens (L.) DC. belongs to the Fabaceae family and is widely distributed across the tropical and subtropical regions of the world. Seeds of Mucuna are the industrially important source of natural L-dopa. The present study is intended to screen out the superior Mucuna germplasm which can be utilized for commercial cultivation. A total of 59 accessions of M. pruriens (L.) DC. were collected from various geographical locations across India and evaluated for seed-associated agronomic traits and L- dopa content in the seed. Morphological observations of seed samples revealed a wide variation in seed color pattern including complete white, black and striped/mottled. Quantitative traits such as 100 seed weight (90.30-150.59gm), seed length (5-19.3 mm), width (2.66-13.00 mm), thickness (0.90–8.67 cm), length width ratio (1.17–2.26), length thickness ratio (1.50–7.40), width thickness ratio (0.89-6.00) seed geometric diameter (2.34–12.60 mm), arithmetic mean diameter (2.88–13.33 mm), seed sphericity (41.89–78.75), seed surface area (17.19-498.51 mm2) and L-dopa content (1.79–6.05%) were evaluated in order to compare the features of the accessions collected from different places. The inter-association between morphological observations and L-dopa content was measured using principal component analysis and Ward’s hierarchical clustering, as these are of vital importance in selecting the best accession of velvet bean for breeding programs.

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Variability for Seed-based Economic Traits and Genetic Diversity Analysis in Mucuna pruriens Population of Northeast India

Cited by 4 publications

References 35 publications

Genetic Diversity Study on Geographical Populations of the Multipurpose Species Elsholtzia stauntonii Using Transferable Microsatellite Markers

Genetic Diversity Study on Geographical Populations of the Multipurpose Species Elsholtzia stauntonii Using Transferable Microsatellite Markers

Bayesian Inference of Soil Traits from Green Manure Fields in a Tropical Sandy Soil

Genetic variability and divergence studies on seed traits and L-dopa content of Mucuna pruriens (L.) DC. accessions

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