Variability among isolates of Fusarium udum and the effect on progression of wilt in pigeonpea

Purohit, Arnab; Ganguly, Sanjib; Ghosh, Gourab; Chaudhuri, Rituparna Kundu; Datta, Siraj; Chakraborti, Dipankar

doi:10.1007/s10658-017-1167-z

Cited by 13 publications

(15 citation statements)

References 12 publications

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“…However, pathogenic isolates and variants were reported in few studies 7 , 8 . In the previous study, present group characterized all the variants reported by Dhar et al 7 and some identified F. udum isolates from MTCC 5 . In the mentioned study, thirteen Indian F. udum isolates were characterized for their pathogenesis in pigeonpea 5 .…”

Section: Discussionmentioning

confidence: 95%

“…F. udum isolates from different geographical locations of India and Kenya were divided into different pathogenic groups based on pathogenicity on different pigeonpea genotypes 7 , 8 . Variations were also observed during pathogenesis and establishment of wilt by different F. udum isolates on susceptible pigeonpea cultivar 5 . Genetic variability among F. udum isolates from various locations of India and Kenya were identified through DNA-based sensitive and precise methods like randomly amplified polymorphic DNA (RAPD) and amplified fragment length polymorphism (AFLP) by several researchers 5 , 7 , 8 .…”

Section: Introductionmentioning

confidence: 97%

“…Variations were also observed during pathogenesis and establishment of wilt by different F. udum isolates on susceptible pigeonpea cultivar 5 . Genetic variability among F. udum isolates from various locations of India and Kenya were identified through DNA-based sensitive and precise methods like randomly amplified polymorphic DNA (RAPD) and amplified fragment length polymorphism (AFLP) by several researchers 5 , 7 , 8 . Additionally, out crossing nature, long life cycle and difficulty in accurate phenotyping were other obstacles of conventional resistance breeding efforts 9 , 10 .…”

Section: Introductionmentioning

confidence: 97%

“…Crop rotation, use of fungicides and development of resistant cultivars are different approaches for management of wilt. Crop rotation does not give complete and durable protection because the fungus can survive in soil for several years, while use of fungicides is not an eco-friendly or economical approach 5 , 6 . Therefore, development of improved cultivars with increased disease resistance is the most sustainable option to manage Fusarium wilt.…”

Section: Introductionmentioning

confidence: 99%

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Comparative transcriptomic profiling of susceptible and resistant cultivars of pigeonpea demonstrates early molecular responses during Fusarium udum infection

Purohit

Ghosh

Ganguly

et al. 2021

Sci Rep

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Vascular wilt caused by Fusarium udum Butler is the most important disease of pigeonpea throughout the world. F. udum isolate MTCC 2204 (M1) inoculated pigeonpea plants of susceptible (ICP 2376) and resistant (ICP 8863) cultivars were taken at invasion stage of pathogenesis process for transcriptomic profiling to understand defense signaling reactions that interplay at early stage of this plant–pathogen encounter. Differential transcriptomic profiles were generated through cDNA-AFLP from M1 inoculated resistant and susceptible pigeonpea root tissues. Twenty five percent of transcript derived fragments (TDFs) were found to be pathogen induced. Among them 73 TDFs were re-amplified and sequenced. Homology search of the TDFs in available databases and thorough study of scientific literature identified several pathways, which could play crucial role in defense responses of the F. udum inoculated resistant plants. Some of the defense responsive pathways identified to be active during this interaction are, jasmonic acid and salicylic acid mediated defense responses, cell wall remodeling, vascular development and pattering, abscisic acid mediated responses, effector triggered immunity, and reactive oxygen species mediated signaling. This study identified important wilt responsive regulatory pathways in pigeonpea which will be helpful for further exploration of these resistant components for pigeonpea improvement.

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Section: Discussionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Comparative transcriptomic profiling of susceptible and resistant cultivars of pigeonpea demonstrates early molecular responses during Fusarium udum infection

Purohit

Ghosh

Ganguly

et al. 2021

Sci Rep

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“…Fusarium oxysporum f. sp. udum , has a number of isolate forms in different pigeonpea growing zones (Purohit et al 2017 ; Padinhare et al 2017 ). However, these isolates have not been characterized at the genome level, but their presence in different growing zones is well known and documented (Naik et al 2017 ).…”

Section: Resultsmentioning

confidence: 99%

A diagnostic marker kit for Fusarium wilt and sterility mosaic diseases resistance in pigeonpea

et al. 2020

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Fusarium wilt (FW) and sterility mosaic diseases (SMD) are key biotic constraints to pigeonpea production. Occurrence of these two diseases in congenial conditions is reported to cause complete yield loss in susceptible pigeonpea cultivars. Various studies to elucidate genomic architecture of the two traits have revealed significant marker–trait associations for use in breeding programs. However, these DNA markers could not be used effectively in genomics-assisted breeding for developing FW and SMD resistant varieties primarily due to pathogen variability, location or background specificity, lesser phenotypic variance explained by the reported QTL and cost-inefficiency of the genotyping assays. Therefore, in the present study, a novel approach has been used to develop a diagnostic kit for identification of suitable FW and SMD resistant lines. This kit was developed with 10 markers each for FW and SMD resistance. Investigation of the diversity of these loci has shown the role of different alleles in different resistant genotypes. Two genes (C.cajan_03691 and C.cajan_18888) for FW resistance and four genes (C.cajan_07858, C.cajan_20995, C.cajan_21801 and C.cajan_17341) for SMD resistance have been identified. More importantly, we developed a customized and cost-effective Kompetitive allele-specific PCR genotyping assay for the identified genes in order to encourage their downstream applications in pigeonpea breeding programs. The diagnostic marker kit developed here will offer great strength to pigeonpea varietal development program, since the resistance against these two diseases is essentially required for nominating an improved line in varietal release pipeline.

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Genetic diversity and population structure of Fusarium udum in India and its correlation with pigeonpea wilt incidence

Deepak Reddy,

Kumar,

Sahni

et al. 2024

J Basic Microbiol

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In a study conducted in India, 50 Fusarium isolates were collected from pigeonpea growing regions and extensively examined for their cultural and morphological characteristics. These isolates exhibited significant variations in traits including growth rate, mycelial growth patterns, color, zonation, pigmentation, spore size, and septation. Subsequently, 30 isolates were chosen for pathogenicity testing on eight pigeonpea genotypes. Results showed distinct reactions, with four genotypes displaying differential responses (ICP8858, ICP8859, ICP8862, and BDN‐2), while ICP9174 and ICP8863 consistently exhibited resistance and ICP2376 and BAHAR remained susceptible to wilt disease. To study the interaction between Fusarium isolates and pigeonpea host differentials (HDs), an additive main effects and multiplicative interaction analysis was conducted. The majority of disease incidence variation (75.54%) was attributed to HD effects, while Fusarium isolate effects accounted for only 1.99%. The interaction between Isolates and HDs (I × HD) contributed 21.95% to the total variation, being smaller than HD but larger than I. Based on HD reactions, isolates were classified into nine variants, showing varying distributions across pigeonpea growing states, with variants 2 and 3 being prevalent in several regions. This diversity underscores the need for location‐specific wilt‐resistant pigeonpea cultivars. Furthermore, genetic analysis of 23 representative isolates, through internal transcribed spacer region of ribosomal DNA and translation elongation factor 1‐α gene sequencing, revealed three major clusters: Fusarium udum, Fusarium solani, and Fusarium equiseti. These findings hold potential for developing location‐specific wilt‐resistant pigeonpea cultivars and enhancing disease management strategies.

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Variability among isolates of Fusarium udum and the effect on progression of wilt in pigeonpea

Cited by 13 publications

References 12 publications

Comparative transcriptomic profiling of susceptible and resistant cultivars of pigeonpea demonstrates early molecular responses during Fusarium udum infection

Comparative transcriptomic profiling of susceptible and resistant cultivars of pigeonpea demonstrates early molecular responses during Fusarium udum infection

A diagnostic marker kit for Fusarium wilt and sterility mosaic diseases resistance in pigeonpea

Genetic diversity and population structure of Fusarium udum in India and its correlation with pigeonpea wilt incidence

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