Variabilité génétique des populations tunisiennes de Myrtus communis L. (Myrtaceae) estimée par des marqueurs isoenzymatiques et moléculaires (RAPD)

Messaoud, Chokri; Makrem, Afif; Boulila, Abdennacer; Rejeb, Mohamed Néjib; Boussaïd, Mohamed

doi:10.1051/forest:2007061

Cited by 19 publications

(9 citation statements)

References 47 publications

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“…These results are in agreement with those reported for forest trees comparing molecular and isozymic data (Aagaard et Messaoud et al, 2007;Yildiray et al, 2007). RAPDs are less responsive to selection and are able to detect high variation both in coding and non-coding regions of the genome (Allnutt et al, 2003;Nybom and Bartish, 2000;Wu et al, 1999).…”

Section: Discussionsupporting

confidence: 83%

Genetic structure of Tunisian natural carob tree (Ceratonia siliqua L.) populations inferred from RAPD markers

et al. 2008

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Abstract• Seven RAPD markers were used to assess the genetic diversity and structure of ten Tunisian natural Ceratonia siliqua L. populations from different geographic and bioclimatic zones.• The species maintain a high diversity within population as estimated by the percentage of polymorphic loci and Shannon's index (P% = 76.31, H pop = 0.569). The range of variation between populations was large. Populations from the upper semi-arid bioclimates, with more continuous distribution area showed the highest level of variation.• A high genetic differentiation among populations (Φ ST = 0.250 and G ST = 0.347), as a result of population isolation was revealed. Nevertheless, the genetic structure is in accordance with bioclimate indicating that ecological factors also should influence differentiation. Populations from the sub-humid, upper semi-arid and mean semi-arid zones clustered together and were distinct from those of the lower semi-arid ones.• Conservation strategy should be made according to the level of polymorphism within population and bioclimate.Mots-clés : diversité génétique / RAPD / Ceratonia siliqua / populations naturelles / bioclimat / conservation Résumé -Structure génétique des populations naturelles du caroubier (Ceratonia siliqua L.) en Tunisie estimée par les marqueurs RAPD.• Sept marqueurs RAPD ont été utilisés pour analyser la diversité et la structure génétique de dix populations tunisiennes de caroubier (Ceratonia siliqua L.) prospectées dans différentes zones géo-graphiques et bioclimatiques.• L'espèce maintient une diversité génétique intra population importante (P% = 76.31 ; H pop = 0.569) et les niveaux de variation diffèrent selon les populations. Celles du subhumide et du semi-aride supérieur, à aire de distribution plus continue, ont montré la variation la plus importante.• Une forte différenciation (Φ ST = 0.250 et G ST = 0.347) entre les populations, due à leur fragmentation récente, est observée. Toutefois, cette différenciation concorde avec le bioclimat des sites témoi-gnant l'influence des facteurs écologiques dans cette différenciation. Les populations du subhumide, du semi-aride supérieur et du semi-aride moyen constituent un groupe distinct de celui représenté par les populations du semi-aride inférieur.• La conservation des populations doit tenir compte de leur niveau de diversité génétique intra population et du bioclimat.

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Section: Discussionsupporting

confidence: 83%

Genetic structure of Tunisian natural carob tree (Ceratonia siliqua L.) populations inferred from RAPD markers

et al. 2008

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“…In the case of Vitellaria paradoxa PD are in general higher with higher level of SSR polymorphism indicating that this species is genetically closer to argan that Manilkara huberi. In addition, these PD values are in general lower than PIC values obtained in the other assays with RAPD as a consequence of the lower polymorphism observed in the application of SSR in comparison with RAPD, as has been previously reported in different species (de-Lucas et al, 2008;Lachenaud and Zhang, 2008;Messaoud et al, 2007;Shiran et al, 2007).…”

Section: Resultsmentioning

confidence: 47%

“…Random amplified polymorphic DNA (RAPD) markers are based on the PCR amplification of random locations in the genome (Messaoud et al, 2007;Welsh and McClelland, 1990). These markers are especially used in the molecular characterization of rare species including argan which are poorly known from the molecular point of view.…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization and genetic relationships among most common identified morphotypes of critically endangered rare Moroccan species Argania spinosa (Sapotaceae) using RAPD and SSR markers

et al. 2008

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. Molecular characterization and genetic relationships among most common identified morphotypes of critically endangered rare Moroccan species Argania spinosa (Sapotaceae) using RAPD and SSR markers. Annals of Forest Science, Springer Verlag/EDP Sciences, 2008, 65 (8), pp.1. Ann. For. Sci. 65 (2008) Abstract• The objective of this work is the molecular characterization of most common identified morphotypes of critically endangered rare Moroccan species Argania spinosa.• Eighteen RAPD markers and twenty SSR markers have been assayed in 38 argan tree accessions from the three most commonly identified morphotypes: oval, spherical and spindle fruit types.• A total of 140 polymorphic RAPD bands were detected out of 146 bands. The number of presumed alleles revealed by the SSR analysis ranged from 1 to 5 alleles per locus with a total number of 32 alleles identified. Results demonstrated an extensive genetic variability within the tested argan accessions. RAPDs presented a high level of polymorphism and greater information content than SSRs.• Our results could indicate that Vitellaria paradoxa is genetically closer to argan than Manilkara huberi. In addition, the correlation between the clustering based on RAPD and SSR markers were in general low. The observed clustering could be better explained according to geographic proximity than morphotype. For this reason the traditional morphological characterization of the argan accessions in morphotypes (according to fruit phenotype) seem not to be in correlation to the real genetic background (genotype) of this specie. The implications of these results in the creation of effective germplasm core collection in argan have been also discussed. Mots-clés :arganier / Argania / collection noyau / relations genetiques / RAPD / Sapotaceae / SSR / taxonomie / transférabilité Résumé -Caractérisation moléculaire et relation génétique entre les morphotypes les plus connus d'une espèce marocaine rare et menacée, Argania spinosa (Sapotaceae) à l'aide de marqueurs RAPD et SSR.• L'objectif de ce travail consiste en la caractérisation moléculaire et l'étude des relations génétiques entre des morphotypes identifiés chez une espèce marocaine rare et menacée, l'arganier (Argania spinosa).• Pour cela, dix-neuf marqueurs RAPD et vingt marqueurs SSR on été testés dans 38 accessions d'arganier des trois morphotypes du fruit identifiés : ovale, sphérique et broche.• En utilisant la technique d'amplification au hasard de l'ADN (RAPD), 140 fragments polymorphes ont été détectés parmi les 146 amplifiés. Le nombre d'allèle révélé par l'analyse des marqueurs microsatelittes (SSRs) varie de un à cinq allèles par locus avec un total de 32 allèles identifiés. Les résultats démontrent une grande variabilité génétique au sein des accessions d'arganier testées. Les marqueurs RAPD présentent un taux de polymorphisme et une hétérozygotie attendue supérieurs aux SSR.• Les résultats indiquent également que Vitellaria paradoxa est plus proche génétiquement de l'arganier que Manilkara huberi. La co...

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“…This slight variation may be caused by primer detection mechanisms: 1) the loci detected by RAPD and ISSR were different; for RAPD, the inverted repeats and internal site were amplified, whereas ISSR amplified inter-segment repeats, and 2) RAPD primers used random sequences of 10 bases, and ISSR primers were based on anchored primer repeats of 15-24 bases; therefore, polymorphism would be different, and the number of polymorphic fragments would increase with the increased number of primers and could also cause the change in genetic distance. Classification results were different by using different technology, as has been published in several studies (Messaoud et al, 2007;Majourhat et al, 2008).…”

Section: Evaluation Of Genetic Similaritymentioning

confidence: 99%

Methodology Analysis of genetic diversity in Larix gmelinii (Pinaceae) with RAPD and ISSR markers

Zhang¹,

Zhang²,

Li³

2013

Genet. Mol. Res.

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ABSTRACT. Dahurian larch (Larix gmelinii), a deciduous conifer, is the northernmost tree, native to eastern Siberia and nearby regions of China. We used growth traits and molecular markers to assess genetic variation in different L. gmelinii growing regions; 105 individual samples were collected from seven regions of the Qingshan Forestry Centre, Heilongjiang Province, China. The greatest genetic regional variation was seen in the Youhao area, based on coefficients of variation for tree height, diameter and volume (14.73, 28.25, and 55.27%, respectively). Analysis using molecular markers showed rich genetic diversity. The RAPD and ISSR methods both indicated that most variation came from within populations. The seven regions were divided into two groups (Daxing'an and Xiaoxing'an Mountain ranges) by RAPD cluster analysis: Tianchi, Xiaojiuya, Yuanjiang, and Taiping regions were placed in the first group at a genetic distance of 0.08; while the other regions were in the second group. The correlation : 196-207 (2013) Genetic diversity of Larix gmelinii between RAPD markers and geographical distance was significant, with a correlation coefficient of 0.752.

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Variabilité génétique des populations tunisiennes de Myrtus communis L. (Myrtaceae) estimée par des marqueurs isoenzymatiques et moléculaires (RAPD)

Cited by 19 publications

References 47 publications

Genetic structure of Tunisian natural carob tree (Ceratonia siliqua L.) populations inferred from RAPD markers

Genetic structure of Tunisian natural carob tree (Ceratonia siliqua L.) populations inferred from RAPD markers

Molecular characterization and genetic relationships among most common identified morphotypes of critically endangered rare Moroccan species Argania spinosa (Sapotaceae) using RAPD and SSR markers

Methodology Analysis of genetic diversity in Larix gmelinii (Pinaceae) with RAPD and ISSR markers

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