VARIABILIDAD GENÉTICA DE CEPAS DE Gallibacterium anatis AISLADAS DE AVES COMERCIALES DEL PERÚ CON INFECCIONES RESPIRATORIAS.

A, Karina Mendoza; Zavaleta, Amparo I.; Koga, Ysabel; B, Jorge Rodríguez; S., Arnaldo Alvarado; R, Robert Tinoco

doi:10.15381/rivep.v25i2.8496

Cited by 4 publications

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“…However, G. anatis has also been associated with a wide range of pathological lesions, especially in the reproductive organs of the egg-laying chicken and is considered a major cause of salpingitis and peritonitis in chickens, leading to lowered egg-production and increased mortality [ 20 – 22 ]. Moreover, G. anatis is globally distributed, having been isolated from poultry in countries within Europe [ 1 , 2 , 4 , 23 ], Africa [ 24 ], Asia [ 25 ], Australia [ 6 ] and the Americas [ 7 , 26 – 29 ].…”

Section: Introductionmentioning

confidence: 99%

Bacterial determinants of importance in the virulence of Gallibacterium anatis in poultry

Persson

Bojesen

2015

Vet Res

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Gallibacterium anatis, a member of the Pasteurellaceae family, constitute a part of the normal micro-flora of the upper respiratory tract and the lower genital tract in chickens. However, increasing evidence indicate that G. anatis is also associated with a wide range of pathological changes, particularly in the reproductive organs, which leads to decreased egg production, lowered animal welfare and increased mortality. As a recently defined opportunistic pathogen limited focus has been placed on the pathogenesis and putative virulence factors permitting G. anatis to cause disease. One of the most studied virulence determinants is a large RTX-like toxin (GtxA), which has been demonstrated to induce a strong leukotoxic effect on avian macrophages. A number of fimbria of different sizes and shapes has been described. Particularly fimbriae belonging to the F17-like family appears to be common in a diverse selection of G. anatis strains. Mutants lacking the FlfA fimbria were severely attenuated in experimentally infected chickens. Additional characteristics including the ability to express capsular material possibly involved in serum resistance; secretion of metalloproteases capable of degrading immunoglobulins, and hemagglutinins, which may promote biofilm formation are all factors likely linked to the virulence of G. anatis. A major advantage for the study of how G. anatis interact with its host is the ability to perform biologically relevant experimental infections where natural routes of exposure allows reproduction of lesions observed during spontaneous infections. This review summarizes the current understanding of the G. anatis pathogenesis and discusses the contribution of the established and putative virulence factors described for this bacterium to date.

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Section: Introductionmentioning

confidence: 99%

Bacterial determinants of importance in the virulence of Gallibacterium anatis in poultry

Persson

Bojesen

2015

Vet Res

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“…Se trabajó con 60 cepas de Gallibacterium sp aisladas de muestras de pollos (BB y de carne), gallinas de postura y gallinas reproductoras con enfermedades respiratorias provenientes de granjas de los departamentos de La Libertad, Ica y Lima (Perú) entre 2014 y 2018. Las cepas se encontraban liofilizadas y guardadas en glicerol a -20 ºC y se tenía registrada la historia clínica del caso y el análisis anatomopatológico (Mendoza et al, 2014).…”

Section: Materiales Y Métodos Materials Biológicounclassified

“…Luego los hisopados e improntas fueron sembrados en placas de agar sangre de ovino al 5 % (las cuales fueron introducidas en campanas de microaerobiosis) y agar MacConkey. Finalmente se incubaron a 37 ºC durante 24-48 horas (Mendoza et al, 2014).…”

Section: Análisis Microbiológicounclassified

“…Los ciclos termales utilizados fueron: 1 ciclo desnaturalización inicial de 95 ºC por 4 min; 35 ciclos: desnatu-ralización a 94 ºC por 30 s; alineamiento a 40 ºC por 3 min y extensión a 72 ºC por 2 min, y finalmente un ciclo de extensión final de 72 ºC por 7 min. Los productos de PCR de ERIC-PCR fueron separados mediante electroforesis en gel de agarosa al 2% en buffer TBE 1X durante 50 min a 100 v y visualizados bajo luz UV (Oliveira et al, 2007;Mendoza et al, 2014).…”

Section: Tipificación Molecular Por Eric-pcrunclassified

“…Esta bacteria es altamente variable, ya que actualmente se describen 24 biovares por pruebas bioquímicas utilizando diferentes azucares (Osuna et al, 2017). Las bacterias altamente variables sufren frecuentemente nuevos cambios genéticos, ya sea por mutaciones debido a su adaptación al medio en que se desarrollan o como consecuencia del intercambio de material genético entre los mismos (Mendoza et al, 2014). Además, el abuso de antibióticos acelera las mutaciones genéticas, adaptándose a los nuevos fármacos (Mendoza et al, 2014).…”

Section: Introductionunclassified

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Genotipificación y fenotipificación de Gallibacterium sp procedentes de aves de corral con signos clínicos respiratorios

Nalvarte¹,

Yanagui²,

Romero³

2020

Rev. investig. vet. Perú

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El objetivo del presente trabajo fue la fenotipificación 60 aislados de Gallibacterium sp mediante un perfil de seis familias de antibióticos y su genotipificación por técnicas de ERIC-PCR, REP-PCR y BOXAIR. Se encontró que el 98.3% de los aislados resisten al menos a una familia de antimicrobianos, y el 11.7% fueron resistentes a las seis familias. La REP-PCR presentó 45 genotipos codificados de R1 hasta R45, la ERIC-PCR presentó 44 genotipos codificados de E1 hasta E44 y la BOXAIR presentó 56 genotipos codificados de B1 hasta B56, dispersos en los departamentos de Lima, Ica y Libertad (Perú). El estudio permitió tipificar Gallibacterium sp y el uso combinado de métodos genotípicos y fenotípicos permitió una discriminación más precisa entre aislamientos potencialmente diferentes con moderada diversidad genética en las ciudades de Lima, Libertad y Ica.

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Gallibacterium anatis infection in poultry: a comprehensive review

Abd El-Ghany,

Algammal,

Hetta

et al. 2023

Trop Anim Health Prod

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Gallibacterium anatis (G. anatis), a member of the Pasteurellaceae family, normally inhabits the upper respiratory and lower genital tracts of poultry. However, under certain circumstances of immunosuppression, co-infection (especially with Escherichia coli or Mycoplasma), or various stressors, G. anatis caused respiratory, reproductive, and systemic diseases. Infection with G. anatis has emerged in different countries worldwide. The bacterium affects mainly chickens; however, other species of domestic and wild birds may get infected. Horizontal, vertical, and venereal routes of G. anatis infection have been reported. The pathogenicity of G. anatis is principally related to the presence of some essential virulence factors such as Gallibacterium toxin A, fimbriae, haemagglutinin, outer membrane vesicles, capsule, biofilms, and protease. The clinical picture of G. anatis infection is mainly represented as tracheitis, oophoritis, salpingitis, and peritonitis, while other lesions may be noted in cases of concomitant infection. Control of such infection depends mainly on applying biosecurity measures and vaccination. The antimicrobial sensitivity test is necessary for the correct treatment of G. anatis. However, the development of multiple drug resistance is common. This review article sheds light on G. anatis regarding history, susceptibility, dissemination, virulence factors, pathogenesis, clinical picture, diagnosis, and control measures.

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VARIABILIDAD GENÉTICA DE CEPAS DE Gallibacterium anatis AISLADAS DE AVES COMERCIALES DEL PERÚ CON INFECCIONES RESPIRATORIAS.

Cited by 4 publications

References 4 publications

Bacterial determinants of importance in the virulence of Gallibacterium anatis in poultry

Bacterial determinants of importance in the virulence of Gallibacterium anatis in poultry

Genotipificación y fenotipificación de Gallibacterium sp procedentes de aves de corral con signos clínicos respiratorios

Gallibacterium anatis infection in poultry: a comprehensive review

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