Phytoplankton biomass (Chl-a) was estimated in 7 stations of Manzanillo Bay, Colima on the surface and at the Secchi depth in the rainy and dry season (2016-2017). To evaluate the biomass, a Millipore equipment and fiberglass GF / F filters were used using the spectrophotometric technique (Lorenzen, 1967). The physicochemical parameters were estimated with a YSI 85 equipment and the nutrients using a San Plus II segmented flow autoanalyzer. The average depth of the Secchi disk ranged from 5.9 m at the Puerto station to 12.8m at the center (A1 and A2). The temperature ranged from 26.9 to 28.1 ° C, the salinity between 31.6 and 33 ups and the dissolved oxygen from 3.81 to 4.82 mg L-1. The nutrients presented high values in Puerto, A1 and Carrizales. The central part of the bay registered values greater than one mg of Chl-a and a maximum of 2.67 mg .m-3 in the Puerto station. In 2016, Chl-a decreased significantly because of a very intense Niño event, also showing high concentrations of phaeopigments, which reveal grazing conditions or degraded chlorophyll. Through an analysis of variance, it was determined that there is a significant difference between the chlorophyll-a values (p≤0.05).