2020
DOI: 10.1016/j.jconrel.2019.12.050
|View full text |Cite
|
Sign up to set email alerts
|

Vapor nanobubble is the more reliable photothermal mechanism for inducing endosomal escape of siRNA without disturbing cell homeostasis

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
3
2

Citation Types

2
50
0

Year Published

2020
2020
2024
2024

Publication Types

Select...
6
2

Relationship

2
6

Authors

Journals

citations
Cited by 48 publications
(52 citation statements)
references
References 40 publications
2
50
0
Order By: Relevance
“…The photothermal effect can lead to endosomal escape mainly by two mechanisms ( Figure 10 B). The first one, known as the heating effect, is a light-activation where a PTA is excited to a high energy level that releases heat to destabilize endo-lysosomal membranes [ 336 ]. The second one occurs at very high heat releasing levels capable of generating a vapor layer surrounding the PTA.…”
Section: Enhancing Ion Endosomal Escapementioning
confidence: 99%
See 1 more Smart Citation
“…The photothermal effect can lead to endosomal escape mainly by two mechanisms ( Figure 10 B). The first one, known as the heating effect, is a light-activation where a PTA is excited to a high energy level that releases heat to destabilize endo-lysosomal membranes [ 336 ]. The second one occurs at very high heat releasing levels capable of generating a vapor layer surrounding the PTA.…”
Section: Enhancing Ion Endosomal Escapementioning
confidence: 99%
“…In this mechanism, the endosomal escape is attributed to mechanical energy dissipation (expansion and collapse of the vapor nanobubbles) and not to the heat diffusion. This has been demonstrated by the negligible heating of the intracellular environment [ 308 , 336 ].…”
Section: Enhancing Ion Endosomal Escapementioning
confidence: 99%
“…mRNA is a large negatively charged, single-stranded nucleic acid that can be encapsulated in synthetic nanocarriers for protection against ubiquitous serum nucleases and enhancing endocytic uptake [17]. Gold nanoparticles, for example, have been extensively studied as drug and gene delivery carriers because of their favorable physicochemical properties [18][19][20][21][22][23][24]. However, carrier-induced cytotoxicity and low transfection efficiency are common disadvantages for T cells [3].…”
Section: Introductionmentioning
confidence: 99%
“…It should be noted, however, that electroporation was amply shown to come with significant loss of cell viability, induction of unwanted phenotypic changes or loss of cell functionality [17,[27][28][29][30]. Laser-assisted photoporation, sometimes also referred to as optoporation, recently came up as a promising gentler technique for intracellular delivery of biological macromolecules [23,24,31]. Wayteck et al, for instance, previously showed in a one-on-one comparison between photoporation and electroporation on murine T cells that a threefold higher percentage of siRNA-transfected viable cells was obtained by photoporation as it induced much less cytotoxicity compared to electroporation [32].…”
Section: Introductionmentioning
confidence: 99%
“…Some lipid formulations used in transfection of siRNAs were shown to be particularly efficient in causing endosomal membrane damage [ 84 , 85 ]. Gold nanoparticles and nanodiamonds were also employed to physically disrupt the endosomal membrane [ 86 , 87 ].…”
Section: Introductionmentioning
confidence: 99%