Vanillin Resistance Induced by BssS Overexpression in Escherichia coli

Ovsienko, M. V.; Федорова, Е. Н.; Doroshenko, V. G.

doi:10.1134/s0003683818010088

Cited by 4 publications

(2 citation statements)

References 21 publications

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“…Therefore, further improved vanillin production can be achieved in E. coli using a vanillin-resistant mutant strain, adsorbent resin that removes vanillin from the fermentation broth, overexpression or engineering of regulators − as well as adopting a dynamic control system to balance growth and production …”

Section: Discussionmentioning

confidence: 99%

Artificial Biosynthetic Pathway for Efficient Synthesis of Vanillin, a Feruloyl-CoA-Derived Natural Product from Eugenol

Zhu,

Wu,

et al. 2024

J. Agric. Food Chem.

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Eugenol, the main component of essential oil from the Syzygium aromaticum clove tree, has great potential as an alternative bioresource feedstock for biosynthesis purposes. Although eugenol degradation to ferulic acid was investigated, an efficient method for directly converting eugenol to targeted natural products has not been established. Herein we identified the inherent inhibitions by simply combining the previously reported ferulic acid biosynthetic pathway and vanillin biosynthetic pathway. To overcome this, we developed a novel biosynthetic pathway for converting eugenol into vanillin, by introducing cinnamoyl-CoA reductase (CCR), which catalyzes conversion of coniferyl aldehyde to feruloyl-CoA. This approach bypasses the need for two catalysts, namely coniferyl aldehyde dehydrogenase and feruloyl-CoA synthetase, thereby eliminating inhibition while simplifying the pathway. To further improve efficiency, we enhanced CCR catalytic efficiency via directed evolution and leveraged an artificialvanillin biosensor for high-throughput screening. Switching the cofactor preference of CCR from NADP+ to NAD+ significantly improved pathway efficiency. This newly designed pathway provides an alternative strategy for efficiently biosynthesizing feruloyl-CoA-derived natural products using eugenol.

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Section: Discussionmentioning

confidence: 99%

Artificial Biosynthetic Pathway for Efficient Synthesis of Vanillin, a Feruloyl-CoA-Derived Natural Product from Eugenol

Zhu,

Wu,

et al. 2024

J. Agric. Food Chem.

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“…Metabolic engineers who use E. coli as their host organism consult EcoCyc to aid in optimizing the production of an end product through a better understanding of the metabolic network and its regulation and to predict undesirable side effects of a metabolic alteration. Metabolic engineering studies using EcoCyc include references 30, 31, 32, 87, 88, and 89.…”

Section: Introductionmentioning

confidence: 99%

The EcoCyc Database

et al. 2018

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EcoCyc is a bioinformatics database available at EcoCyc.org that describes the genome and the biochemical machinery of Escherichia coli K-12 MG1655. The long-term goal of the project is to describe the complete molecular catalog of the E. coli cell, as well as the functions of each of its molecular parts, to facilitate a systems-level understanding of E. coli. EcoCyc is an electronic reference source for E. coli biologists and for biologists who work with related microorganisms. The database includes information pages on each E. coli gene product, metabolite, reaction, operon, and metabolic pathway. The database also includes information on E. coli gene essentiality and on nutrient conditions that do or do not support the growth of E. coli. The website and downloadable software contain tools for analysis of high-throughput data sets. In addition, a steady-state metabolic flux model is generated from each new version of EcoCyc and can be executed via EcoCyc.org. The model can predict metabolic flux rates, nutrient uptake rates, and growth rates for different gene knockouts and nutrient conditions. This review outlines the data content of EcoCyc and of the procedures by which this content is generated.

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