Vanilla mosaic virus isolates from French Polynesia and the Cook Islands are Dasheen mosaic virus strains that exclusively infect vanilla

Farreyrol, Karin; Pearson, M. N.; Grisoni, Michel; Cohen, Daniel; Beck, David L.

doi:10.1007/s00705-005-0680-0

Cited by 18 publications

(4 citation statements)

References 48 publications

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“…The identities between these viruses and the dendrogram clustering of these viruses together (Fig. 3) clearly indicates the close relationship among these viruses, as was also reported earlier (Adams et al, 2005;Reyes et al, 2009;Farreyrol et al, 2006). DsMV isolates from the three edible aroids under study differed from two other DsMV isolates (AY994104, AY994105) and with one VMV (AJ616719) from GenBank, in lacking 36 nucleotides at the 5'-end region of the CP and 54 nucleotides (140-194) compared to 9 DsMV isolates (AM910398, DMU00122, AF169832, EF199550, DQ925464, AM910407, AM910401, AM994104, and AY994105) and one VMV isolate (AJ616719) (data not shown).…”

Section: Resultssupporting

confidence: 85%

Molecular characterization of dasheen mosaic virus isolates infecting edible aroids in India

Babu¹,

Hegde²

2014

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Summary. -Dasheen mosaic virus (DsMV) infecting three major edible aroids namely Amorphophallus paeoniifolius, Colocasia esculenta, and Xanthosoma sagittifolium cultivated in India was characterized. Infected plants showing typical DsMV symptoms were subjected to reverse transcription-polymerase chain reaction, and an amplification of a 963 bp fragment which encoded the coat protein (CP) gene was obtained. BLAST analysis of the cloned DNA amplicon revealed the identity of the virus to be that of DsMV. Sequence identity matrix of the nucleotide sequences among the three isolates showed that the DsMV isolate infecting A. paeoniifolius and C. esculenta shared an identity as high as 93%, while the DsMV isolate from X. sagittifolium shared an identity of only 73% and 76% with the DsMV isolates from A. paeoniifolius and C. esculenta, respectively. Comparative analysis of the coat protein of the three DsMV isolates showed the presence of DVG motif (A. paeoniifolius and C. esculenta) and DTG motif in X. sagittifolium and several varying potential threonine and asparagine rich N-glycosylation motifs. Single amino acid substitution of the several conserved motifs occurs in all the three DsMV isolates. This is the first characterization of DsMV isolates infecting A. paeoniifolius, C. esculenta, and X. sagittifolium plants in India.

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Section: Resultssupporting

confidence: 85%

Molecular characterization of dasheen mosaic virus isolates infecting edible aroids in India

Babu¹,

Hegde²

2014

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“…The opposite situation was described for vanilla mosaic virus (VanMV), which was firstly considered as a distinct species but further research allowed for its classification as a DsMV strain that exclusively infects Vanilla fragrans Ames. (Wang and Pearson 1992;Farreyrol et al 2006). It indicates that further studies are required regarding DsMV differentiation and adaptation to different hosts.…”

Section: Discussionmentioning

confidence: 99%

Journal of Plant Protection Research

2022

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Dasheen mosaic virus (DsMV) is one of the most important viral pathogens of aroids and can cause major economic losses for ornamental crops. Here, we present the detection and molecular characterisation of DsMV isolates originating from Monstera adansonii plants in Poland. Moreover, the genetic variability of DsMV isolates was analyzed based on the coat protein gene (CP) of the Polish and other DsMV isolates described to date. The presence of DsMV was confirmed by transmission electron microscopy (TEM) and reverse transcription polymerase chain reaction (RT-PCR) with specific, diagnostic primers in three out of ten examined plants. To obtain full-length sequences of CP, two pairs of primers were designed and used in the RT-PCR. The specificity of obtained products was confirmed by Sanger sequencing. The obtained sequences of CP were compared with 44 other DsMV sequences retrieved from the GenBank. Analyses revealed that DsMV population is very diverse. The variability of DsMV isolates was confirmed by low sequence identity and pervasive recombination events. The phylogenetic analysis was performed based on 37 non-recombinant CP sequences. The maximum-likelihood reconstruction revealed that the Polish isolates are distinct and grouped separately from other DsMV isolates. Due to the high genetic diversity, detecting the virus could be difficult. Nonetheless disease management relies strongly on a fast and accurate identification of the causal agent. To our knowledge this is the first report of DsMV in Poland.

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“…Thus, although the mutation is associated with the insertion, it also occurs in isolates without an insertion. The question, therefore, arises of whether this mutation, previously reported for potyviruses [19][20][21], has an impact on the features of the CP and on the aphid transmission.…”

Section: Discussionmentioning

confidence: 99%

Genetic diversity of Hungarian Maize dwarf mosaic virus isolates

2009

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The genetic diversity of the coat-protein (CP) region and the untranslated C-terminal region (3'UTR) of Maize dwarf mosaic virus (MDMV) was analyzed to evaluate the variability between isolates (inter-isolate sequence diversity). The results of inter-isolate sequence diversity analysis showed that the diversity of the MDMV CP gene is fairly high (p-distance: up to 0.136). During sequence analysis, a 13 amino-acid residue insertion and an 8 amino-acid residue deletion were found within the N-terminal region of the CP gene. The phylogenetic analysis showed that-unlike other potyvirus species in this subgroup-the MDMV isolates could not be distinguished on the basis of their host plants or geographic origins.

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Vanilla mosaic virus isolates from French Polynesia and the Cook Islands are Dasheen mosaic virus strains that exclusively infect vanilla

Cited by 18 publications

References 48 publications

Molecular characterization of dasheen mosaic virus isolates infecting edible aroids in India

Molecular characterization of dasheen mosaic virus isolates infecting edible aroids in India

Journal of Plant Protection Research

Genetic diversity of Hungarian Maize dwarf mosaic virus isolates

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