Vancomycin resistant enterococci: From the hospital effluent to the urban wastewater treatment plant

Varela, Ana Rita; Ferro, Giovanna; Vredenburg, Jana; Yanık, Melike; Vieira, Lucas Guedes; Rizzo, Luigi; Lameiras, Catarina; Manaia, Célia M.

doi:10.1016/j.scitotenv.2013.02.015

Cited by 105 publications

(68 citation statements)

References 35 publications

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“…And, the role of hospital effluents as critical antibiotic resistance reservoirs has been demonstrated in different studies that compared antibiotic resistance prevalence in hospital and municipal wastewater. These studies demonstrated that hospital effluents contain higher prevalence of ARGs (e.g., tetW, blaTEM, or sul1), including some genetic determinants which occurrence is mainly related with clinical practices, such as the gene vanA (Varela et al 2013(Varela et al , 2015bNarcisoda-Rocha et al 2014;Rodriguez-Mozaz et al 2015). Hospital effluents can contain higher concentrations of antibiotic residues, metalloids, and metals like arsenic or mercury, and significantly higher abundance of ARB&ARG than municipal effluents (Kümmerer and Henninger 2003;Varela et al 2014;Rodriguez-Mozaz et al 2015).…”

Section: Important Environmental Reservoirs Of Contaminant Antibioticmentioning

confidence: 99%

“…This legal gap can be attributed to the fact that hospital effluent discharges represent less than 5 % of the municipal effluents, leading to the readily dilution of antibiotic residues and ARB in the domestic effluents Rodriguez-Mozaz et al 2015). How-ever, this may be a fallacious argument, since ARB&ARG, unlike other environmental contaminants, can proliferate and spread across different niches and hosts, as it has been demonstrated based on the detection of the closely related ARG&ARB in urban water streams, gulls, or hospitalized patients (Varela et al 2013(Varela et al , 2015bVredenburg et al 2014). In particular, the effluents from hospital and health care units can represent important reservoirs of ARG associated with bacteria of clinical relevance and with antibiotics used mainly in hospitals (ECDC 2012).…”

Section: Important Environmental Reservoirs Of Contaminant Antibioticmentioning

confidence: 99%

See 1 more Smart Citation

Antibiotic resistance in urban aquatic environments: can it be controlled?

Manaia

Macedo

Fatta‐Kassinos

et al. 2015

Appl Microbiol Biotechnol

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175

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Over the last decade, numerous evidences have contributed to establish a link between the natural and human-impacted environments and the growing public health threat that is the antimicrobial resistance. In the environment, in particular in areas subjected to strong anthropogenic pressures, water plays a major role on the transformation and transport of contaminants including antibiotic residues, antibiotic-resistant bacteria, and antibiotic resistance genes. Therefore, the urban water cycle, comprising water abstraction, disinfection, and distribution for human consumption, and the collection, treatment, and delivery of wastewater to the environment, is a particularly interesting loop to track the fate of antibiotic resistance in the environment and to assess the risks of its trans-mission back to humans. In this article, the relevance of different transepts of the urban water cycle on the potential enrichment and spread of antibiotic resistance is reviewed. According to this analysis, some gaps of knowledge, research needs, and control measures are suggested. The critical rationale behind the measures suggested and the desirable involvement of some key action players is also discussed.

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Section: Important Environmental Reservoirs Of Contaminant Antibioticmentioning

confidence: 99%

Section: Important Environmental Reservoirs Of Contaminant Antibioticmentioning

confidence: 99%

Antibiotic resistance in urban aquatic environments: can it be controlled?

Manaia

Macedo

Fatta‐Kassinos

et al. 2015

Appl Microbiol Biotechnol

Self Cite

175

View full text Add to dashboard Cite

show abstract

“…Similarly, no VRE were detected in surface waters by Rathnayake et al (2012), in unchlorinated water samples by Wilson & McAfee (2002) and in river samples, municipal and hospital wastewaters by Servais & Passerat (2009). Conversely, a study performed by Varela et al (2013) demonstrated the detection of vancomycin-resistant enterococci from hospital and urban wastewater samples. Again, the VRE prevalence was recorded as 12.9% in the aquatic environmental samples in Korea by Nam et al (2013) and as 25.6% in superficial water samples by Messi et al (2006).…”

Section: Resultsmentioning

confidence: 94%

“…Besides being the hygiene quality indicator for water, enterococci have been proposed as indicator bacteria for antimicrobial resistance (Boehm & Sassoubre 2014). Vancomycin is a antibiotic, strongly affects Gram-positive bacteria for the treatment of serious, life-threatening infections, when other antibiotic treatment did not work (Varela et al, 2013). Vancomycin resistance in enterococci have been occured all over the world since 1986 (Cetinkaya et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Investigation of the Presence and Virulence Traits of Vancomycin-Resistant Enterococcus in Water Samples

Muş¹

2017

J Food Health Sci

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Abstract:The aim of this study was to determine the incidence of vancomycin-resistant enterococci (VRE) in tap and artesian well waters and to detect the vancomycin resistance genes and virulence genes of the isolates obtained from the samples. For this purpose, 200 samples (119 tap and 81 artesian well waters) were collected from several water supplies during November 2013 and June 2015 period in Bursa province. Seven isolates were recovered from artesian well waters and confirmed as Enterococcus by PCR method. E-test performed for vancomycin and teicoplanin MIC values indicated that only two isolates had the intermediate-level (8 μg/mL) resistance to vancomycin. No resistance was observed to teicoplanin in any of these isolates by Etest. All of 7 isolates were tested for vancomycin resistance genes (vanA, vanB and vanC) and virulence genes (gelE, agg, esp and ace). The results showed that enterococci isolates had no these genes. The present study suggested that the presence of the intermediate level VRE in artesian well waters and, also the waters from environmental supplies near human and animal niches could be play a role as potential reservoirs for enterococci having several types of resistance to vancomycin. Also, vancomycin resistant strains can be possible the spread in environment and also the transmission to human and animals through contaminated water sources.

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“…In an investigation of eight livestock farms in Greece, the prevalence of VRE carrying vanA in broilers was 14.4% [40]. VRE have also been detected during the wastewater treatment process and in hospital effluents in the US [41,42]. It is likely that the excessive use of glycopeptide antibiotics such as vancomycin in healthcare facilities has resulted in the selective increase in VRE in the human intestine.…”

Section: Discussionmentioning

confidence: 99%

Antibiotic Resistance Profiling and Genotyping of Vancomycin-Resistant Enterococci Collected from an Urban River Basin in the Provincial City of Miyazaki, Japan

Nishiyama

Ogura

Hayashi

et al. 2017

Water

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Abstract:The distribution characteristics of vancomycin-resistant enterococci (VRE) and the resistance of enterococcus isolates to various antibiotics were investigated in Yae River, which flows through Miyazaki city, Japan. The prevalence of VRE among specimens collected from the urban river basin using mEI agar was 0.9% (2 of 226 enterococcal isolates). In the 333 enterococcal isolates obtained using mEI agar or vancomycin-supplemented mEI agar, the possession of the vancomycin-resistant genes (vanA, vanB, vanC1, and vanC2/C3) was examined using multiplex PCR analysis. Although VRE possessing vanA and vanB were not detected in any isolates, isolates possessing vanC2/C3 were detected at all sampling sites and on all days. All isolates (101 strains) possessing vanC2/C3 that were obtained on vancomycin-supplemented mEI agar were identified as E. casseliflavus and analyzed for genotypes using pulse-field gel electrophoresis (PFGE) analysis. These E. casseliflavus isolates revealed them to be genetically highly divergent strains, suggesting that many contamination sources were present in this study area. Many of the enterococcal isolates obtained were resistant to erythromycin, ciprofloxacin, and tetracycline; enterococci distributed in the studied urban river basin are resistant to universally applicable antibiotics. These results indicate that VRE carrying vanC2/C3 are distributed in Yae River, and the sources of VRE are scattered across the river basin.

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Vancomycin resistant enterococci: From the hospital effluent to the urban wastewater treatment plant

Cited by 105 publications

References 35 publications

Antibiotic resistance in urban aquatic environments: can it be controlled?

Antibiotic resistance in urban aquatic environments: can it be controlled?

Investigation of the Presence and Virulence Traits of Vancomycin-Resistant Enterococcus in Water Samples

Antibiotic Resistance Profiling and Genotyping of Vancomycin-Resistant Enterococci Collected from an Urban River Basin in the Provincial City of Miyazaki, Japan

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